Team:Evry/Interlab Study/09-11-2014

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         <li> PCR colony of 3 colonies for each construction following protocol table 1 and 2.
         <li> PCR colony of 3 colonies for each construction following protocol table 1 and 2.
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<i>PSB1C3+E0240+J23100</i>, <i>PSB1C3+E0240+J23102</i>, <i>PSB1C3+E0240+J23103</i>, <i>PSB1C3+E0240+J23104</i> and <i>PSB1C3+E0240+J23118</i>,<br>
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        <li> PCR colony of 1 colony for each construction following protocol table 1 and 2.
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    <center>GEL 1: 1% agarose gel. Lane 1: PCR product of 1 colonies of the PSB1C3+E0240+J23100 construction.  Lane 2: PCR product of 1 colonies of the PSB1C3+E0240+J23102 construction. Lane 3: PCR product of 1 colonies of the PSB1C3+E0240+J23103 construction. Lane 4: PCR product of 1 colonies of the PSB1C3+E0240+J23104 construction. </center>
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<span class="cd-date">Sep 11</span>  
<span class="cd-date">Sep 11</span>  

Revision as of 16:36, 8 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101, PSB1C3+E0240+J23118, PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:
  • Transformation plate from the 10th September observation. There were 50 colonies for each constructions.


PSB1C3+E0240+J23101 and PSB1C3+E0240+J23118,
  • PCR colony of 3 colonies for each construction following protocol table 1 and 2.
    IMAGE
    Symbol
    GEL 1: 1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction. Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction.
  • PCR purification of sample 1 and 4 of the Gel 1 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
  • Preparation of samples to sequencing. N° XX
  • Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.
PSB1C3+E0240+J23100, PSB1C3+E0240+J23102, PSB1C3+E0240+J23103, PSB1C3+E0240+J23104 and PSB1C3+E0240+J23118,
  • PCR colony of 1 colony for each construction following protocol table 1 and 2.
    IMAGE
    Symbol
    GEL 1: 1% agarose gel. Lane 1: PCR product of 1 colonies of the PSB1C3+E0240+J23100 construction. Lane 2: PCR product of 1 colonies of the PSB1C3+E0240+J23102 construction. Lane 3: PCR product of 1 colonies of the PSB1C3+E0240+J23103 construction. Lane 4: PCR product of 1 colonies of the PSB1C3+E0240+J23104 construction.

    Sep 11

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