Team:Evry/Interlab Study/09-18-2014

From 2014.igem.org

(Difference between revisions)
Line 31: Line 31:
<i>PSB1C3+E0240+J23101</i>: <br>  
<i>PSB1C3+E0240+J23101</i>: <br>  
<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
-
<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).
+
<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).<br>
<img src="" alt="PLAN DE PLAQUE" />
<img src="" alt="PLAN DE PLAQUE" />
<br>
<br>
Line 38: Line 38:
<i>PSB1C3+E0240+J23102</i>: <br>  
<i>PSB1C3+E0240+J23102</i>: <br>  
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
-
<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).
+
<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30). <br>
<img src="" alt="PLAN DE PLAQUE" />
<img src="" alt="PLAN DE PLAQUE" />
<br>
<br>

Revision as of 14:55, 8 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
PLAN DE PLAQUE

Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).
    PLAN DE PLAQUE

    PSB1C3+E0240+J23102:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).
    PLAN DE PLAQUE

    PSB1C3+E0240+J23104:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23105:
    glycerol stock preparation from LB culture of the 17th September.

    PSB1C3+E0240+J23107:
    glycerol stock preparation from LB culture of the 17th September.

    PSB1C3+E0240+J23110:
    Pre-culture does'nt grow. The TECAN measure is reported.

    PSB1C3+E0240+J23115:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23116:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23117:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
    Sep 18

    • Retrieved from "http://2014.igem.org/Team:Evry/Interlab_Study/09-18-2014"