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Team:Cambridge-JIC/Marchantia/EnhancerTrap
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| - | How does an Enhancer Trap work? | + | <h3>How does an Enhancer Trap work?</h3> |
An enhancer trap constructs contains a transcription activator (e.g.: GAL4) fused to a weak or basal promoter (TATA box). The construct also contains a reporter gene (e.g.: YFP) fused to a UAS (upstream activator sequence activated by GLA4). This allows YFP to report the GAL4 expression. On its own the basal promoter is insufficient to drive detectable expression of the reporter gene, but can respond to transcriptional enhancers from the genome it is inserted next to.. Once a transgenic line is generated using such a construct it can be crossed with any other transgenic line containing a UAS::GOI (gene of interest). The offspring will express the gene of interest with the same characteristic seen as the reporter gene (e.g.: YFP). | An enhancer trap constructs contains a transcription activator (e.g.: GAL4) fused to a weak or basal promoter (TATA box). The construct also contains a reporter gene (e.g.: YFP) fused to a UAS (upstream activator sequence activated by GLA4). This allows YFP to report the GAL4 expression. On its own the basal promoter is insufficient to drive detectable expression of the reporter gene, but can respond to transcriptional enhancers from the genome it is inserted next to.. Once a transgenic line is generated using such a construct it can be crossed with any other transgenic line containing a UAS::GOI (gene of interest). The offspring will express the gene of interest with the same characteristic seen as the reporter gene (e.g.: YFP). | ||
Revision as of 12:54, 8 October 2014
How does an Enhancer Trap work?
An enhancer trap constructs contains a transcription activator (e.g.: GAL4) fused to a weak or basal promoter (TATA box). The construct also contains a reporter gene (e.g.: YFP) fused to a UAS (upstream activator sequence activated by GLA4). This allows YFP to report the GAL4 expression. On its own the basal promoter is insufficient to drive detectable expression of the reporter gene, but can respond to transcriptional enhancers from the genome it is inserted next to.. Once a transgenic line is generated using such a construct it can be crossed with any other transgenic line containing a UAS::GOI (gene of interest). The offspring will express the gene of interest with the same characteristic seen as the reporter gene (e.g.: YFP).
