Team:ULB-Brussels/OurBrick
From 2014.igem.org
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<h3> Characterization </h3> | <h3> Characterization </h3> | ||
- | <p> In order to characterize the ccdB biobrick, we sent the biobricks to sequencing and made a screen of activity for the protein ccdB. We did a $\small killing$ $\small assay$, because of the toxic property of ccdB.<br><br> | + | <p> In order to characterize the ccdB biobrick, we sent the biobricks to sequencing and made a $\small screen$ $\small of$ $\small activity$ for the protein ccdB. We did a $\small killing$ $\small assay$, because of the toxic property of ccdB.<br><br> |
- | We constructed 4 different colonies including one with the plasmid pKK-233-ccda, another with pBAD33-ccdB, a third with both, and a control colony. The ccdA gene encoded for a protein wich acts as an anti-toxin of ccdB.<br><br> | + | We constructed $\small 4$ $\small different$ $\small colonies$ including one with the plasmid pKK-233-ccda, another with pBAD33-ccdB, a third with both, and a control colony. The ccdA gene encoded for a protein wich acts as an anti-toxin of ccdB.<br><br> |
- | On the first media containing IPTG (inducing the pKK233’s expression) and glucose (repressing the pBAD expression), each colony grew. That allowed us to control the non toxicity of ccdA. | + | On the first media containing IPTG (inducing the pKK233’s expression) and glucose (repressing the pBAD's expression), each colony grew. That allowed us to control the non toxicity of ccdA. |
- | On the media containing both IPTG and arabinose, the strand with pBAD is killed and the strand with both ccdA | + | On the media containing both IPTG and arabinose (inducing the pBAD's expression), the strand with pBAD is killed and the strand with both ccdA $small\&$ ccdB grew.<br><br> |
We made dilution to assure that the cell concentration didn’t affect the toxicity or the anti-toxicity. </p> | We made dilution to assure that the cell concentration didn’t affect the toxicity or the anti-toxicity. </p> |
Revision as of 15:53, 7 October 2014
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$
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