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Team:ULB-Brussels/OurBrick
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<section style="text-align: justify; margin: 50px"> | <section style="text-align: justify; margin: 50px"> | ||
| - | < | + | <h1> Our Part </h1> |
| - | + | <h3> Properties </h3> | |
| - | + | <p> Host: E.Coli <br> | |
| + | Source: ccdBA operon <br> | ||
| + | Plasmid: available using> i>LabGenius BioBrick Mapper</i> <br> | ||
| + | Length: 309 bp <br> | ||
| + | </p> | ||
Our <a href="http://parts.igem.org/Part:BBa_K1318000"> ULB-Brussels part </a> | Our <a href="http://parts.igem.org/Part:BBa_K1318000"> ULB-Brussels part </a> | ||
</section> | </section> | ||
| + | <section style="text-align: justify; margin: 50px"> | ||
<h3> Characterization </h3> | <h3> Characterization </h3> | ||
| - | <p>In order to characterize the ccdB biobrick, we sent the biobricks to sequencing and made a screen of activity for the protein ccdB. We did a $\small killing$ $\small assay$, because of the toxic property of ccdB.<br> | + | <p> In order to characterize the ccdB biobrick, we sent the biobricks to sequencing and made a screen of activity for the protein ccdB. We did a $\small killing$ $\small assay$, because of the toxic property of ccdB.<br><br> |
| - | We constructed 4 different colonies including one with the plasmid pKK-233-ccda, another with pBAD33-ccdB, a third with both, and a control colony. The ccdA gene encoded for a protein wich acts as an anti-toxin of ccdB.<br> | + | We constructed 4 different colonies including one with the plasmid pKK-233-ccda, another with pBAD33-ccdB, a third with both, and a control colony. The ccdA gene encoded for a protein wich acts as an anti-toxin of ccdB.<br><br> |
On the first media containing IPTG (inducing the pKK233’s expression) and glucose (repressing the pBAD expression), each colony grew. That allowed us to control the non toxicity of ccdA. | On the first media containing IPTG (inducing the pKK233’s expression) and glucose (repressing the pBAD expression), each colony grew. That allowed us to control the non toxicity of ccdA. | ||
| - | On the media containing both IPTG and arabinose, the strand with pBAD is killed and the strand with both ccdA and ccdB grew.<br> | + | On the media containing both IPTG and arabinose, the strand with pBAD is killed and the strand with both ccdA and ccdB grew.<br><br> |
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| + | We made dilution to assure that the cell concentration didn’t affect the toxicity or the anti-toxicity. </p> | ||
| + | </section> | ||
</td> | </td> | ||
Revision as of 15:44, 7 October 2014
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$
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- Université Libre de Bruxelles -
