Team:TCU Taiwan/Notebook

experimental part:

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α).
Incubate DH5α with pSB1C3.

Extract plasmid with pSB1C3 from DH5α.

other part:

experimental part:

other part:

Get foundation from nearby store (yeah!!!!!)
Decided to have iGEM contest in local school as human practice.

Others don’t think E.Maat is a good name, under re-consideration.

experimental part:

Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.

Prepare Cm. plate、LB. plate.

other part:

Something wrong with gRNA for AmpR, redesign.

Second project theme came out by Denise and Regina: Trogene horse. Needs later discuss to decided which one as theme( E.Maat or Trogene Horse).

Try to get foundation from Biotechnology company on e-mail.

Prepare for igem conference in NCTU-Formosa (ppt).

experimental part:

Incubate E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 ).

Extract pSB1C3 BBa_I13521、BBa_ K914003、BBa_ K1218011.

other part:

Final Theme decided: Trogene Horse.

Prepare for igem conference in NCTU-Formosa (ppt).

Designing team logo and project logo.

House started working on wiki.

Decided to have a synthetic biology contest in National Hualien Girls’ Senior High School.

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )

2. E.coli top10 with pBluescript II SK (-)

Extract :

1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

other part:

Prepare for igem conference in NCTU-Formosa (ppt).

Designing team logo and project logo.

House working on wiki construction.

Prepare for the contest in National Hualien Girls’ Senior High School (ppt).

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 ).

2. E.coli top10 with pBluescript II SK (-).

Extract :

1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

other part:

Prepare for igem conference in NCTU-Formosa (ppt).

Have a synthetic biology contest in National Hualien Girls’ Senior High School.

Order primer for gRNA team logo and project logo confirmed.

Attending iGEM conference in NCTU-Formosa.

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )

2. E.coli top10 with pBluescript II SK (-)

Extract :

1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

Purification of RFP sequence from pSB1C3 Prepare Cm. plate、LB. plate.

other part:

Team uniform under design.

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )

2. E.coli top10 with pBluescript II SK (-)

Extract :

1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

Purification of RFP sequence from pSB1C3.

experimental part:

Ligation of RFP and pBluescript SK(-).

Transformation clone RFP/pBluescript SK(-) to DH5α.

Extract plasmid clone RFP/pBluescript SK(-).

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

other part:

Design badge as small gift.

Order Helper phage for pBluescript SK(-).

Make movie for project introduction of Trogene horse.

Prepare iGEM presentation ppt、poster.

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、(in pSB1C3 )

2. E.coli top10 with pBluescript II SK (-)

Extract :

1. pSB1C3: BBa_I13521

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

Purification of RFP sequence from pSB1C3.

Ligation Clone RFP/pBluescript SK(-)to DH5α Store BBa_I13521、BBa_ K914003、BBa_ K1218011、clone RFP/pBluescript SK(-).

other part:

Make movie for project introduction of Trogene.

horse Prepare iGEM presentation ppt、poster.

experimental part:

Ligation Clone RFP/pBluescript SK(-).

Extract plasmid clone RFP/pBluescript SK(-).

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

Modeling for phage injection (Thanks for NCTU-Formosa’s help).

other part:

experimental part:

Ligation Clone RFP/pBluescript SK(-).

Extractplasmid clone RFP/pBluescript SK(-).

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

Prepare competent cell(JM101).

incubate:

1. E.coli DH5α with BBa_I13521(pBluescript)、BBa_K1473001~3003(in pSB1C3 )

Extract :

1. pSB1C3: BBa_I13521、BBa_K1473001~3003

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

PCR: tracrRNA、Cas 9+crRNA.

Modeling for phage injection.

other part:

Prepare iGEM presentation ppt、poster.

Wiki construction.

experimental part:

Ligation gRNA/ pSB1C3.

Transformation gRNA/ pSB1C3 to DH5α.

Incubate gRNA/ pSB1C3 in E.coli JM101.

Extract plasmid gRNA/ pSB1C3 in E.coli JM101.

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

PCR trRNA、Cas 9+crRNA.

Prepare modeling.

3 new biobrick finished.

other part:

Have a iGEM contest(HP) in Tzu chi university.

Prepare presentation ppt、poster.

Wiki construction.

other part:

Prepare iGEM presentation ppt、poster.

Wiki construction.