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Team:EPF Lausanne/Parts
From 2014.igem.org
(Difference between revisions)
| Line 189: | Line 189: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486012</td> | <td class="biobrick_name">BBa_K1486012</td> | ||
| - | <td>CpxR + | + | <td>CpxR + IFP[1]</td> |
<td>CpxR with the Nterm moiety of IFP attached at its C terminus</td> | <td>CpxR with the Nterm moiety of IFP attached at its C terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
| Line 196: | Line 196: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486013</td> | <td class="biobrick_name">BBa_K1486013</td> | ||
| - | <td>CpxR + | + | <td>CpxR + IFP[2]</td> |
<td>CpxR with the Cterm moiety of IFP attached at its C terminus</td> | <td>CpxR with the Cterm moiety of IFP attached at its C terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
| Line 203: | Line 203: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486014</td> | <td class="biobrick_name">BBa_K1486014</td> | ||
| - | <td> | + | <td>IFP[1] + CpxR</td> |
<td>CpxR with the Nterm moiety of IFP attached at its N terminus</td> | <td>CpxR with the Nterm moiety of IFP attached at its N terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
| Line 210: | Line 210: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486015</td> | <td class="biobrick_name">BBa_K1486015</td> | ||
| - | <td> | + | <td>IFP[2] + CpxR</td> |
<td>CpxR with the Cterm moiety of IFP attached at its N terminus</td> | <td>CpxR with the Cterm moiety of IFP attached at its N terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
| Line 217: | Line 217: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486016</td> | <td class="biobrick_name">BBa_K1486016</td> | ||
| - | <td> | + | <td>fLuc[1]</td> |
<td>N terminus moiety of the firefly luciferase</td> | <td>N terminus moiety of the firefly luciferase</td> | ||
<td> </td> | <td> </td> | ||
| Line 224: | Line 224: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486017</td> | <td class="biobrick_name">BBa_K1486017</td> | ||
| - | <td> | + | <td>fLuc[2]</td> |
<td>C terminus moiety of the firefly luciferase</td> | <td>C terminus moiety of the firefly luciferase</td> | ||
<td> </td> | <td> </td> | ||
| Line 231: | Line 231: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486018</td> | <td class="biobrick_name">BBa_K1486018</td> | ||
| - | <td>PAra + | + | <td>PAra + fLuc[1] + fLuc[2]</td> |
<td>Split firefly luciferase under arabinose promoter</td> | <td>Split firefly luciferase under arabinose promoter</td> | ||
<td>Control</td> | <td>Control</td> | ||
| Line 238: | Line 238: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486019</td> | <td class="biobrick_name">BBa_K1486019</td> | ||
| - | <td> | + | <td>rLuc[1]</td> |
<td>C terminus moiety of the renilla luciferase</td> | <td>C terminus moiety of the renilla luciferase</td> | ||
<td> </td> | <td> </td> | ||
| Line 245: | Line 245: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486020</td> | <td class="biobrick_name">BBa_K1486020</td> | ||
| - | <td> | + | <td>rLuc[2]</td> |
<td>N terminus moiety of the renilla luciferase</td> | <td>N terminus moiety of the renilla luciferase</td> | ||
<td> </td> | <td> </td> | ||
| Line 252: | Line 252: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486021</td> | <td class="biobrick_name">BBa_K1486021</td> | ||
| - | <td>PAra + | + | <td>PAra + rLuc[1] + rLuc[2]</td> |
<td>Split renilla luciferase under arabinose promoter</td> | <td>Split renilla luciferase under arabinose promoter</td> | ||
<td>Control</td> | <td>Control</td> | ||
| Line 301: | Line 301: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486028</td> | <td class="biobrick_name">BBa_K1486028</td> | ||
| - | <td> | + | <td>Yeast sfGFP[1]</td> |
<td>N terminal moiety of split yeast sfGFP</td> | <td>N terminal moiety of split yeast sfGFP</td> | ||
<td> </td> | <td> </td> | ||
| Line 308: | Line 308: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486029</td> | <td class="biobrick_name">BBa_K1486029</td> | ||
| - | <td> | + | <td>sfGFP[1] + kan</td> |
<td>Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene</td> | <td>Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene</td> | ||
<td> </td> | <td> </td> | ||
| Line 315: | Line 315: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486030</td> | <td class="biobrick_name">BBa_K1486030</td> | ||
| - | <td> | + | <td>rLuc[1] + kan</td> |
<td>Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene</td> | <td>Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene</td> | ||
<td> </td> | <td> </td> | ||
| Line 343: | Line 343: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486034</td> | <td class="biobrick_name">BBa_K1486034</td> | ||
| - | <td> | + | <td>yeast sfGFP[2]</td> |
<td>C terminal moiety of split the yeast sfGFP</td> | <td>C terminal moiety of split the yeast sfGFP</td> | ||
<td> </td> | <td> </td> | ||
| Line 350: | Line 350: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486035</td> | <td class="biobrick_name">BBa_K1486035</td> | ||
| - | <td> | + | <td>yeast sfGFP[2] + Ura</td> |
<td>Cterm moiety of split yeast sfGFP attached to the Uracil CDS</td> | <td>Cterm moiety of split yeast sfGFP attached to the Uracil CDS</td> | ||
<td> </td> | <td> </td> | ||
| Line 357: | Line 357: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486036</td> | <td class="biobrick_name">BBa_K1486036</td> | ||
| - | <td> | + | <td>rLuc[2] + Ura</td> |
<td>Cterm moiety of split renilla luciferase attached to the Uracil CDS</td> | <td>Cterm moiety of split renilla luciferase attached to the Uracil CDS</td> | ||
<td> </td> | <td> </td> | ||
| Line 371: | Line 371: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486038</td> | <td class="biobrick_name">BBa_K1486038</td> | ||
| - | <td> | + | <td>sfGFP[1]</td> |
<td>N terminus moiety of split superfolder GFP</td> | <td>N terminus moiety of split superfolder GFP</td> | ||
<td> </td> | <td> </td> | ||
| Line 378: | Line 378: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486039</td> | <td class="biobrick_name">BBa_K1486039</td> | ||
| - | <td> | + | <td>sfGFP[2]</td> |
<td>C terminus moiety of split superfolder GFP</td> | <td>C terminus moiety of split superfolder GFP</td> | ||
<td> </td> | <td> </td> | ||
| Line 385: | Line 385: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486040</td> | <td class="biobrick_name">BBa_K1486040</td> | ||
| - | <td> | + | <td>sfGFP[1] + CpxR</td> |
<td>N terminus moiety of split sfGFP attached to CpxR</td> | <td>N terminus moiety of split sfGFP attached to CpxR</td> | ||
<td> </td> | <td> </td> | ||
| Line 392: | Line 392: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486041</td> | <td class="biobrick_name">BBa_K1486041</td> | ||
| - | <td> | + | <td>sfGFP[2] + CpxR</td> |
<td>C terminus moiety of split sfGFP attached to CpxR</td> | <td>C terminus moiety of split sfGFP attached to CpxR</td> | ||
<td> </td> | <td> </td> | ||
| Line 413: | Line 413: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486044</td> | <td class="biobrick_name">BBa_K1486044</td> | ||
| - | <td> | + | <td>mut IFP[1]</td> |
| - | <td> | + | <td>Biobrick-compatible IFP[1]</td> |
<td> </td> | <td> </td> | ||
| - | <td> | + | <td>Bacteria</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486045</td> | <td class="biobrick_name">BBa_K1486045</td> | ||
| - | <td> | + | <td>mut IFP[2]</td> |
| - | <td> | + | <td>Biobrick-compatible IFP[2]</td> |
<td> </td> | <td> </td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
| Line 427: | Line 427: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486046</td> | <td class="biobrick_name">BBa_K1486046</td> | ||
| - | <td> | + | <td>CpxR promoter FW</td> |
| - | <td> | + | <td>CpxR binding-region in forward direction</td> |
<td> </td> | <td> </td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
| Line 434: | Line 434: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486047</td> | <td class="biobrick_name">BBa_K1486047</td> | ||
| - | <td> | + | <td>CpxR promoter RV</td> |
| - | <td> | + | <td>CpxR binding-region in reverse direction</td> |
<td> </td> | <td> </td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
| Line 441: | Line 441: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486048</td> | <td class="biobrick_name">BBa_K1486048</td> | ||
| - | <td> | + | <td>CpxR reporter</td> |
| - | <td> | + | <td>Calgary's CpxR reporter repaired (sequence was missing)</td> |
| - | <td> </td> | + | <td>To see when CpxR is active</td> |
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486049</td> | <td class="biobrick_name">BBa_K1486049</td> | ||
| - | <td> | + | <td>CpxR promoter FW + RFP</td> |
| - | <td> | + | <td>Reporter of CpxR</td> |
| - | <td> </td> | + | <td>Test the direction of the complete CpxR promoter</td> |
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486050</td> | <td class="biobrick_name">BBa_K1486050</td> | ||
| - | <td> | + | <td>CpxR promoter RV + RFP</td> |
| - | <td> | + | <td>Reporter of CpxR</td> |
| - | <td> | + | <td>Test the direction of the complete CpxR promoter</td> |
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486051</td> | <td class="biobrick_name">BBa_K1486051</td> | ||
| - | <td> | + | <td>CheY</td> |
| - | <td> | + | <td>Quorum sensing protein, dimerizes with CheZ</td> |
| - | <td> | + | <td> </td> |
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486052</td> | <td class="biobrick_name">BBa_K1486052</td> | ||
| - | <td> | + | <td>CheZ</td> |
| - | <td> | + | <td>Quorum sensing protein, dimerizes with CheY</td> |
| - | <td> | + | <td> </td> |
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486053</td> | <td class="biobrick_name">BBa_K1486053</td> | ||
| - | <td> | + | <td>Linker</td> |
| - | <td> | + | <td>10 amino-acid linker</td> |
| - | <td> | + | <td>Attach CheY/Z to split luciferases</td> |
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486054</td> | <td class="biobrick_name">BBa_K1486054</td> | ||
| - | <td> | + | <td>CheY/CheZ rLuc</td> |
| - | <td> | + | <td>CheY and CheZ, each attached to a moiety of split renilla luciferase</td> |
| - | <td> | + | <td>Positive control for the split rLuc</td> |
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486055</td> | <td class="biobrick_name">BBa_K1486055</td> | ||
| - | <td> | + | <td>CheY/CheZ fLuc</td> |
| - | <td> | + | <td>CheY and CheZ, each attached to a moiety of split firefly luciferase</td> |
| - | <td> | + | <td>Positive control for the split fLuc</td> |
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486056</td> | <td class="biobrick_name">BBa_K1486056</td> | ||
| - | <td> | + | <td>CxpR & Split mut IFP1.4 [Cterm + Cterm]</td> |
| - | <td>Two | + | <td>Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP</td> |
| - | <td> | + | <td>Characterize CpxR dimerization</td> |
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
Revision as of 14:46, 7 October 2014
PARTS
Parts submitted by the 2014 EPFL iGEM team
Our team submitted a total of 43 Biobricks.
| Biobrick | What it is | Function | Why do we use it? | Group |
|---|---|---|---|---|
| BBa_K1486000 | CpxR coding sequence | Transcription factor | To make most of our biobricks! | Bacteria |
| BBa_K1486001 | CpxR under arabinose promoter | Treanscription factor | Bacteria | |
| BBa_K1486002 | PAra + sfGFP CpxR [Nterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486003 | Flexible linker | Attaches two proteins together | Bacteria | |
| BBa_K1486004 | Flexible linker | Attaches two proteins together | Bacteria | |
| BBa_K1486005 | PAra + CpxR sfGFP [Cterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486006 | IFP[1] | N terminus of split IFP | Bacteria | |
| BBa_K1486007 | IFP[2] | C terminus of split IFP | Bacteria | |
| BBa_K1486008 | CxpR & Split IFP1.4 [Cterm + Cterm] | Two CpxR CDS, each C terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486009 | CxpR & Split IFP1.4 [Nterm + Nterm] | Two CpxR CDS, each N terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486010 | CxpR & Split IFP1.4 [Nterm + Cterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486011 | CxpR & Split IFP1.4 [Cterm + Nterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486012 | CpxR + IFP[1] | CpxR with the Nterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486013 | CpxR + IFP[2] | CpxR with the Cterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486014 | IFP[1] + CpxR | CpxR with the Nterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486015 | IFP[2] + CpxR | CpxR with the Cterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486016 | fLuc[1] | N terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486017 | fLuc[2] | C terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486018 | PAra + fLuc[1] + fLuc[2] | Split firefly luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486019 | rLuc[1] | C terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486020 | rLuc[2] | N terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486021 | PAra + rLuc[1] + rLuc[2] | Split renilla luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486022 | rLuc | Full renilla luciferase | Control | Bacteria |
| BBa_K1486023 | Yeast sfGFP | Superfolder GFP for yeast cells | Reporter | Yeast |
| BBa_K1486024 | Kan | Yeast kanamycin resistance gene | Selection marker | Yeast |
| BBa_K1486025 | ADH1 terminator | Terminator | Yeast | |
| BBa_K1486026 | Yeast sfGFP + Kan | Yeast sfGFP attached to the yeast kanamycin resistance gene | Control the expression of pbs2 | Yeast |
| BBa_K1486027 | rLuc + Kan | Renilla luciferase attached to the kanamycin resistance gene | Yeast | |
| BBa_K1486028 | Yeast sfGFP[1] | N terminal moiety of split yeast sfGFP | Yeast | |
| BBa_K1486029 | sfGFP[1] + kan | Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene | Yeast | |
| BBa_K1486030 | rLuc[1] + kan | Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene | Yeast | |
| BBa_K1486031 | Ura | CDS for Uracil (yeast selective purposes) | Confer resistance to Uracil-deprived medium | Yeast |
| BBa_K1486032 | Yeast sfGFP + Ura | Yeast sfGFP attached to the Uracil CDS | Control the expression of hog1 | Yeast |
| BBa_K1486033 | rLuc + Ura | Renilla luciferase attached to the Uracil CDS | Control the expression of hog1 | Yeast |
| BBa_K1486034 | yeast sfGFP[2] | C terminal moiety of split the yeast sfGFP | Yeast | |
| BBa_K1486035 | yeast sfGFP[2] + Ura | Cterm moiety of split yeast sfGFP attached to the Uracil CDS | Yeast | |
| BBa_K1486036 | rLuc[2] + Ura | Cterm moiety of split renilla luciferase attached to the Uracil CDS | Yeast | |
| BBa_K1486037 | linker | Attaches two proteins together | Yeast | |
| BBa_K1486038 | sfGFP[1] | N terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486039 | sfGFP[2] | C terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486040 | sfGFP[1] + CpxR | N terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486041 | sfGFP[2] + CpxR | C terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486042 | LZip | Monomer of leucine zipper TF | Bacteria | |
| BBa_K1486043 | LZip + split rLuc | Two Leucine Zipper monomers, each attached to a different split rLuc moiety | Control for split rLuc assays | Bacteria |
| BBa_K1486044 | mut IFP[1] | Biobrick-compatible IFP[1] | Bacteria | |
| BBa_K1486045 | mut IFP[2] | Biobrick-compatible IFP[2] | Bacteria | |
| BBa_K1486046 | CpxR promoter FW | CpxR binding-region in forward direction | Bacteria | |
| BBa_K1486047 | CpxR promoter RV | CpxR binding-region in reverse direction | Bacteria | |
| BBa_K1486048 | CpxR reporter | Calgary's CpxR reporter repaired (sequence was missing) | To see when CpxR is active | Bacteria |
| BBa_K1486049 | CpxR promoter FW + RFP | Reporter of CpxR | Test the direction of the complete CpxR promoter | Bacteria |
| BBa_K1486050 | CpxR promoter RV + RFP | Reporter of CpxR | Test the direction of the complete CpxR promoter | Bacteria |
| BBa_K1486051 | CheY | Quorum sensing protein, dimerizes with CheZ | Bacteria | |
| BBa_K1486052 | CheZ | Quorum sensing protein, dimerizes with CheY | Bacteria | |
| BBa_K1486053 | Linker | 10 amino-acid linker | Attach CheY/Z to split luciferases | Bacteria |
| BBa_K1486054 | CheY/CheZ rLuc | CheY and CheZ, each attached to a moiety of split renilla luciferase | Positive control for the split rLuc | Bacteria |
| BBa_K1486055 | CheY/CheZ fLuc | CheY and CheZ, each attached to a moiety of split firefly luciferase | Positive control for the split fLuc | Bacteria |
| BBa_K1486056 | CxpR & Split mut IFP1.4 [Cterm + Cterm] | Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP | Characterize CpxR dimerization | Bacteria |
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