Team:TCU Taiwan/Notebook

From 2014.igem.org

(Difference between revisions)
Line 252: Line 252:
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 2</font><font face="Trebuchet MS" size="6" color="#90B849"> 6/29~7/5</font></h6>       
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 2</font><font face="Trebuchet MS" size="6" color="#90B849"> 6/29~7/5</font></h6>       
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Successfully transform biobrick BBa_I13521、BBa_ K914003、BBa_ K1218011 into DH5α (plasmid: pSB1C3<br>Extract pSB1C3 after incubation<br>Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 is successfully extracted<br>Prepared Cm. plate、LB. plate<br>
 +
Prepared competent cell
 +
</font></p>
 +
          <p>&nbsp;</p>
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 3</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/6~7/12</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 3</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/6~7/12</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <p><br>
 +
            <font face="Trebuchet MS" size="4" color="#333">Get foundation from nearby store (yeah!!!!!)<br>Decided to have iGEM contest in local school as human practice</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Others don’t think E.Maat is a good name, under re-consideration </font></p>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 4</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/13~7/19</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 4</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/13~7/19</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
       <h6 align="left"> <font face="Trebuchet MS" size="6" color="#A66B38">Week 5</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/20~7/26</font></h6>
       <h6 align="left"> <font face="Trebuchet MS" size="6" color="#A66B38">Week 5</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/20~7/26</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 6</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/27~8/2</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 6</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/27~8/2</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 7</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/3~8/7</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 7</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/3~8/7</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 8</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/10~16</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 8</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/10~16</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 9</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/17~8/23</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 9</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/17~8/23</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 10</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/24~8/30</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 10</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/24~8/30</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 11</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/31~9/6</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 11</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/31~9/6</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 12</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/7~9/13</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 12</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/7~9/13</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 13</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/14~9/20</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 13</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/14~9/20</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 14</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/21~9/27</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 14</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/21~9/27</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
          
          
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 15</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/28~10/4</font></h6>
         <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 15</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/28~10/4</font></h6>
-
         <div><font face="Trebuchet MS" size="4" color="#A66B38">Test</font></div>
+
         <div align="left">
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
 +
            Transformation ( Cas9 in pSB1C3 into DH5α)<br>
 +
            Incubate DH5α with pSB1C3</font></p>
 +
          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font></p>
 +
          <p>         
 +
          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
 +
          <br>
 +
          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>
 +
          Practice presentation<br>
 +
          Design gRNA for AmpR<br>
 +
Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
 +
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font>
 +
          </p>
 +
        </div>
     </div>
     </div>
</div></td>
</div></td>

Revision as of 14:12, 7 October 2014

 
Notebook
  

Notebook

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

   
Week 1 6/22~6/28

experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 2 6/29~7/5
experimental part:

Successfully transform biobrick BBa_I13521、BBa_ K914003、BBa_ K1218011 into DH5α (plasmid: pSB1C3
Extract pSB1C3 after incubation
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 is successfully extracted
Prepared Cm. plate、LB. plate
Prepared competent cell

 

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 3 7/6~7/12
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:


Get foundation from nearby store (yeah!!!!!)
Decided to have iGEM contest in local school as human practice

Others don’t think E.Maat is a good name, under re-consideration

Week 4 7/13~7/19
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 5 7/20~7/26
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 6 7/27~8/2
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 7 8/3~8/7
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 8 8/10~16
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 9 8/17~8/23
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 10 8/24~8/30
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 11 8/31~9/6
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 12 9/7~9/13
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 13 9/14~9/20
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 14 9/21~9/27
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 15 9/28~10/4
experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

^
    

^

Retrieved from "http://2014.igem.org/Team:TCU_Taiwan/Notebook"