"
Team:EPF Lausanne/Parts
From 2014.igem.org
(Difference between revisions)
| Line 114: | Line 114: | ||
<td>CpxR under arabinose promoter</td> | <td>CpxR under arabinose promoter</td> | ||
<td>Treanscription factor</td> | <td>Treanscription factor</td> | ||
| + | <td> </td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
| - | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 128: | Line 128: | ||
<td>Flexible linker</td> | <td>Flexible linker</td> | ||
<td>Attaches two proteins together</td> | <td>Attaches two proteins together</td> | ||
| + | <td> </td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
| - | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 135: | Line 135: | ||
<td>Flexible linker</td> | <td>Flexible linker</td> | ||
<td>Attaches two proteins together</td> | <td>Attaches two proteins together</td> | ||
| + | <td> </td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
| - | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 149: | Line 149: | ||
<td>IFP[1]</td> | <td>IFP[1]</td> | ||
<td>N terminus of split IFP</td> | <td>N terminus of split IFP</td> | ||
| + | <td> </td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
| - | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 156: | Line 156: | ||
<td>IFP[2]</td> | <td>IFP[2]</td> | ||
<td>C terminus of split IFP</td> | <td>C terminus of split IFP</td> | ||
| + | <td> </td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
| - | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 171: | Line 171: | ||
<td>Two CpxR CDS, each N terminus attached to a moiety of IFP</td> | <td>Two CpxR CDS, each N terminus attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 177: | Line 178: | ||
<td>Two CpxR CDS, each attached to a moiety of IFP</td> | <td>Two CpxR CDS, each attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 183: | Line 185: | ||
<td>Two CpxR CDS, each attached to a moiety of IFP</td> | <td>Two CpxR CDS, each attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 189: | Line 192: | ||
<td>CpxR with the Nterm moiety of IFP attached at its C terminus</td> | <td>CpxR with the Nterm moiety of IFP attached at its C terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 195: | Line 199: | ||
<td>CpxR with the Cterm moiety of IFP attached at its C terminus</td> | <td>CpxR with the Cterm moiety of IFP attached at its C terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 201: | Line 206: | ||
<td>CpxR with the Nterm moiety of IFP attached at its N terminus</td> | <td>CpxR with the Nterm moiety of IFP attached at its N terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 207: | Line 213: | ||
<td>CpxR with the Cterm moiety of IFP attached at its N terminus</td> | <td>CpxR with the Cterm moiety of IFP attached at its N terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 212: | Line 219: | ||
<td>Nterm fLuc</td> | <td>Nterm fLuc</td> | ||
<td>N terminus moiety of the firefly luciferase</td> | <td>N terminus moiety of the firefly luciferase</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 218: | Line 226: | ||
<td>Cterm fLuc</td> | <td>Cterm fLuc</td> | ||
<td>C terminus moiety of the firefly luciferase</td> | <td>C terminus moiety of the firefly luciferase</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 225: | Line 234: | ||
<td>Split firefly luciferase under arabinose promoter</td> | <td>Split firefly luciferase under arabinose promoter</td> | ||
<td>Control</td> | <td>Control</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 230: | Line 240: | ||
<td>Nterm rLuc</td> | <td>Nterm rLuc</td> | ||
<td>C terminus moiety of the renilla luciferase</td> | <td>C terminus moiety of the renilla luciferase</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 236: | Line 247: | ||
<td>Cterm rLuc</td> | <td>Cterm rLuc</td> | ||
<td>N terminus moiety of the renilla luciferase</td> | <td>N terminus moiety of the renilla luciferase</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 243: | Line 255: | ||
<td>Split renilla luciferase under arabinose promoter</td> | <td>Split renilla luciferase under arabinose promoter</td> | ||
<td>Control</td> | <td>Control</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 249: | Line 262: | ||
<td>Full renilla luciferase</td> | <td>Full renilla luciferase</td> | ||
<td>Control</td> | <td>Control</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 255: | Line 269: | ||
<td>Superfolder GFP for yeast cells</td> | <td>Superfolder GFP for yeast cells</td> | ||
<td>Reporter</td> | <td>Reporter</td> | ||
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 261: | Line 276: | ||
<td>Yeast kanamycin resistance gene</td> | <td>Yeast kanamycin resistance gene</td> | ||
<td>Selection marker</td> | <td>Selection marker</td> | ||
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 266: | Line 282: | ||
<td>ADH1 terminator</td> | <td>ADH1 terminator</td> | ||
<td>Terminator</td> | <td>Terminator</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 273: | Line 290: | ||
<td>Yeast sfGFP attached to the yeast kanamycin resistance gene</td> | <td>Yeast sfGFP attached to the yeast kanamycin resistance gene</td> | ||
<td>Control the expression of pbs2</td> | <td>Control the expression of pbs2</td> | ||
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486027</td> | <td class="biobrick_name">BBa_K1486027</td> | ||
<td>rLuc + Kan</td> | <td>rLuc + Kan</td> | ||
| - | <td>Renilla luciferase attached to the | + | <td>Renilla luciferase attached to the kanamycin resistance gene</td> |
| - | <td></td> | + | <td> </td> |
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 284: | Line 303: | ||
<td>Nterm Yeast sfGFP</td> | <td>Nterm Yeast sfGFP</td> | ||
<td>N terminal moiety of split yeast sfGFP</td> | <td>N terminal moiety of split yeast sfGFP</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 290: | Line 310: | ||
<td>Nterm sfGFP + kan</td> | <td>Nterm sfGFP + kan</td> | ||
<td>Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene</td> | <td>Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 296: | Line 317: | ||
<td>Nterm rLuc + kan</td> | <td>Nterm rLuc + kan</td> | ||
<td>Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene</td> | <td>Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 303: | Line 325: | ||
<td>CDS for Uracil (yeast selective purposes)</td> | <td>CDS for Uracil (yeast selective purposes)</td> | ||
<td>Confer resistance to Uracil-deprived medium</td> | <td>Confer resistance to Uracil-deprived medium</td> | ||
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 309: | Line 332: | ||
<td>Yeast sfGFP attached to the Uracil CDS</td> | <td>Yeast sfGFP attached to the Uracil CDS</td> | ||
<td>Control the expression of hog1</td> | <td>Control the expression of hog1</td> | ||
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 315: | Line 339: | ||
<td>Renilla luciferase attached to the Uracil CDS</td> | <td>Renilla luciferase attached to the Uracil CDS</td> | ||
<td>Control the expression of hog1</td> | <td>Control the expression of hog1</td> | ||
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 320: | Line 345: | ||
<td>Cterm yeast sfGFP</td> | <td>Cterm yeast sfGFP</td> | ||
<td>C terminal moiety of split the yeast sfGFP</td> | <td>C terminal moiety of split the yeast sfGFP</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 326: | Line 352: | ||
<td>Cterm yeast sfGFP + Ura</td> | <td>Cterm yeast sfGFP + Ura</td> | ||
<td>Cterm moiety of split yeast sfGFP attached to the Uracil CDS</td> | <td>Cterm moiety of split yeast sfGFP attached to the Uracil CDS</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 332: | Line 359: | ||
<td>Cterm rLuc + Ura</td> | <td>Cterm rLuc + Ura</td> | ||
<td>Cterm moiety of split renilla luciferase attached to the Uracil CDS</td> | <td>Cterm moiety of split renilla luciferase attached to the Uracil CDS</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 338: | Line 366: | ||
<td>linker</td> | <td>linker</td> | ||
<td>Attaches two proteins together</td> | <td>Attaches two proteins together</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 344: | Line 373: | ||
<td>Nterm sfGFP</td> | <td>Nterm sfGFP</td> | ||
<td>N terminus moiety of split superfolder GFP</td> | <td>N terminus moiety of split superfolder GFP</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 350: | Line 380: | ||
<td>Cterm sfGFP</td> | <td>Cterm sfGFP</td> | ||
<td>C terminus moiety of split superfolder GFP</td> | <td>C terminus moiety of split superfolder GFP</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 356: | Line 387: | ||
<td>Nterm sfGFP + CpxR</td> | <td>Nterm sfGFP + CpxR</td> | ||
<td>N terminus moiety of split sfGFP attached to CpxR</td> | <td>N terminus moiety of split sfGFP attached to CpxR</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 362: | Line 394: | ||
<td>Cterm sfGFP + CpxR</td> | <td>Cterm sfGFP + CpxR</td> | ||
<td>C terminus moiety of split sfGFP attached to CpxR</td> | <td>C terminus moiety of split sfGFP attached to CpxR</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 368: | Line 401: | ||
<td>LZip</td> | <td>LZip</td> | ||
<td>Monomer of leucine zipper TF</td> | <td>Monomer of leucine zipper TF</td> | ||
| - | <td></td> | + | <td> </td> |
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 375: | Line 409: | ||
<td>Two Leucine Zipper monomers, each attached to a different split rLuc moiety</td> | <td>Two Leucine Zipper monomers, each attached to a different split rLuc moiety</td> | ||
<td>Control for split rLuc assays</td> | <td>Control for split rLuc assays</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 17:51, 6 October 2014
PARTS
Parts submitted by the 2014 EPFL iGEM team
Our team submitted a total of 43 Biobricks.
| Biobrick | What it is | Function | Why do we use it? | Group |
|---|---|---|---|---|
| BBa_K1486000 | CpxR coding sequence | Transcription factor | To make most of our biobricks! | Bacteria |
| BBa_K1486001 | CpxR under arabinose promoter | Treanscription factor | Bacteria | |
| BBa_K1486002 | PAra + sfGFP CpxR [Nterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486003 | Flexible linker | Attaches two proteins together | Bacteria | |
| BBa_K1486004 | Flexible linker | Attaches two proteins together | Bacteria | |
| BBa_K1486005 | PAra + CpxR sfGFP [Cterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486006 | IFP[1] | N terminus of split IFP | Bacteria | |
| BBa_K1486007 | IFP[2] | C terminus of split IFP | Bacteria | |
| BBa_K1486008 | CxpR & Split IFP1.4 [Cterm + Cterm] | Two CpxR CDS, each C terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486009 | CxpR & Split IFP1.4 [Nterm + Nterm] | Two CpxR CDS, each N terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486010 | CxpR & Split IFP1.4 [Nterm + Cterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486011 | CxpR & Split IFP1.4 [Cterm + Nterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486012 | CpxR + Nterm IFP | CpxR with the Nterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486013 | CpxR + Cterm IFP | CpxR with the Cterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486014 | Nterm IFP + CpxR | CpxR with the Nterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486015 | Cterm IFP + CpxR | CpxR with the Cterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486016 | Nterm fLuc | N terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486017 | Cterm fLuc | C terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486018 | PAra + Nterm fLuc + Cterm fLuc | Split firefly luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486019 | Nterm rLuc | C terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486020 | Cterm rLuc | N terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486021 | PAra + Nterm rLuc + Cterm rLuc | Split renilla luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486022 | rLuc | Full renilla luciferase | Control | Bacteria |
| BBa_K1486023 | Yeast sfGFP | Superfolder GFP for yeast cells | Reporter | Yeast |
| BBa_K1486024 | Kan | Yeast kanamycin resistance gene | Selection marker | Yeast |
| BBa_K1486025 | ADH1 terminator | Terminator | Yeast | |
| BBa_K1486026 | Yeast sfGFP + Kan | Yeast sfGFP attached to the yeast kanamycin resistance gene | Control the expression of pbs2 | Yeast |
| BBa_K1486027 | rLuc + Kan | Renilla luciferase attached to the kanamycin resistance gene | Yeast | |
| BBa_K1486028 | Nterm Yeast sfGFP | N terminal moiety of split yeast sfGFP | Yeast | |
| BBa_K1486029 | Nterm sfGFP + kan | Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene | Yeast | |
| BBa_K1486030 | Nterm rLuc + kan | Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene | Yeast | |
| BBa_K1486031 | Ura | CDS for Uracil (yeast selective purposes) | Confer resistance to Uracil-deprived medium | Yeast |
| BBa_K1486032 | Yeast sfGFP + Ura | Yeast sfGFP attached to the Uracil CDS | Control the expression of hog1 | Yeast |
| BBa_K1486033 | rLuc + Ura | Renilla luciferase attached to the Uracil CDS | Control the expression of hog1 | Yeast |
| BBa_K1486034 | Cterm yeast sfGFP | C terminal moiety of split the yeast sfGFP | Yeast | |
| BBa_K1486035 | Cterm yeast sfGFP + Ura | Cterm moiety of split yeast sfGFP attached to the Uracil CDS | Yeast | |
| BBa_K1486036 | Cterm rLuc + Ura | Cterm moiety of split renilla luciferase attached to the Uracil CDS | Yeast | |
| BBa_K1486037 | linker | Attaches two proteins together | Yeast | |
| BBa_K1486038 | Nterm sfGFP | N terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486039 | Cterm sfGFP | C terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486040 | Nterm sfGFP + CpxR | N terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486041 | Cterm sfGFP + CpxR | C terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486042 | LZip | Monomer of leucine zipper TF | Bacteria | |
| BBa_K1486043 | LZip + split rLuc | Two Leucine Zipper monomers, each attached to a different split rLuc moiety | Control for split rLuc assays | Bacteria |
Sponsors
Copyright © iGEM EPFL 2014. All Rights Reserved
