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Team:EPF Lausanne/Parts
From 2014.igem.org
(Difference between revisions)
| Line 101: | Line 101: | ||
<th>Function</th> | <th>Function</th> | ||
<th>Why do we use it?</th> | <th>Why do we use it?</th> | ||
| + | <th>Group</th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 107: | Line 108: | ||
<td>Transcription factor</td> | <td>Transcription factor</td> | ||
<td>To make most of our biobricks!</td> | <td>To make most of our biobricks!</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 112: | Line 114: | ||
<td>CpxR under arabinose promoter</td> | <td>CpxR under arabinose promoter</td> | ||
<td>Treanscription factor</td> | <td>Treanscription factor</td> | ||
| + | <td>Bacteria</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
| Line 119: | Line 122: | ||
<td>Expresses fused protein</td> | <td>Expresses fused protein</td> | ||
<td>Test CpxR expression & Ara promoter</td> | <td>Test CpxR expression & Ara promoter</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 124: | Line 128: | ||
<td>Flexible linker</td> | <td>Flexible linker</td> | ||
<td>Attaches two proteins together</td> | <td>Attaches two proteins together</td> | ||
| + | <td>Bacteria</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
| Line 130: | Line 135: | ||
<td>Flexible linker</td> | <td>Flexible linker</td> | ||
<td>Attaches two proteins together</td> | <td>Attaches two proteins together</td> | ||
| + | <td>Bacteria</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
| Line 137: | Line 143: | ||
<td>Expresses fused protein</td> | <td>Expresses fused protein</td> | ||
<td>Test CpxR expression & Ara promoter</td> | <td>Test CpxR expression & Ara promoter</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 142: | Line 149: | ||
<td>IFP[1]</td> | <td>IFP[1]</td> | ||
<td>N terminus of split IFP</td> | <td>N terminus of split IFP</td> | ||
| + | <td>Bacteria</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
| Line 148: | Line 156: | ||
<td>IFP[2]</td> | <td>IFP[2]</td> | ||
<td>C terminus of split IFP</td> | <td>C terminus of split IFP</td> | ||
| + | <td>Bacteria</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
| Line 155: | Line 164: | ||
<td>Two CpxR CDS, each C terminus attached to a moiety of IFP</td> | <td>Two CpxR CDS, each C terminus attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
| + | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 17:46, 6 October 2014
PARTS
Parts submitted by the 2014 EPFL iGEM team
Our team submitted a total of 43 Biobricks.
| Biobrick | What it is | Function | Why do we use it? | Group |
|---|---|---|---|---|
| BBa_K1486000 | CpxR coding sequence | Transcription factor | To make most of our biobricks! | Bacteria |
| BBa_K1486001 | CpxR under arabinose promoter | Treanscription factor | Bacteria | |
| BBa_K1486002 | PAra + sfGFP CpxR [Nterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486003 | Flexible linker | Attaches two proteins together | Bacteria | |
| BBa_K1486004 | Flexible linker | Attaches two proteins together | Bacteria | |
| BBa_K1486005 | PAra + CpxR sfGFP [Cterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486006 | IFP[1] | N terminus of split IFP | Bacteria | |
| BBa_K1486007 | IFP[2] | C terminus of split IFP | Bacteria | |
| BBa_K1486008 | CxpR & Split IFP1.4 [Cterm + Cterm] | Two CpxR CDS, each C terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486009 | CxpR & Split IFP1.4 [Nterm + Nterm] | Two CpxR CDS, each N terminus attached to a moiety of IFP | Characterize CpxR dimerization | |
| BBa_K1486010 | CxpR & Split IFP1.4 [Nterm + Cterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | |
| BBa_K1486011 | CxpR & Split IFP1.4 [Cterm + Nterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | |
| BBa_K1486012 | CpxR + Nterm IFP | CpxR with the Nterm moiety of IFP attached at its C terminus | Intermediate & control | |
| BBa_K1486013 | CpxR + Cterm IFP | CpxR with the Cterm moiety of IFP attached at its C terminus | Intermediate & control | |
| BBa_K1486014 | Nterm IFP + CpxR | CpxR with the Nterm moiety of IFP attached at its N terminus | Intermediate & control | |
| BBa_K1486015 | Cterm IFP + CpxR | CpxR with the Cterm moiety of IFP attached at its N terminus | Intermediate & control | |
| BBa_K1486016 | Nterm fLuc | N terminus moiety of the firefly luciferase | ||
| BBa_K1486017 | Cterm fLuc | C terminus moiety of the firefly luciferase | ||
| BBa_K1486018 | PAra + Nterm fLuc + Cterm fLuc | Split firefly luciferase under arabinose promoter | Control | |
| BBa_K1486019 | Nterm rLuc | C terminus moiety of the renilla luciferase | ||
| BBa_K1486020 | Cterm rLuc | N terminus moiety of the renilla luciferase | ||
| BBa_K1486021 | PAra + Nterm rLuc + Cterm rLuc | Split renilla luciferase under arabinose promoter | Control | |
| BBa_K1486022 | rLuc | Full renilla luciferase | Control | |
| BBa_K1486023 | Yeast sfGFP | Superfolder GFP for yeast cells | Reporter | |
| BBa_K1486024 | Kan | Yeast kanamycin resistance gene | Selection marker | |
| BBa_K1486025 | ADH1 terminator | Terminator | ||
| BBa_K1486026 | Yeast sfGFP + Kan | Yeast sfGFP attached to the yeast kanamycin resistance gene | Control the expression of pbs2 | |
| BBa_K1486027 | rLuc + Kan | Renilla luciferase attached to the yeast kanamycin resistance gene | ||
| BBa_K1486028 | Nterm Yeast sfGFP | N terminal moiety of split yeast sfGFP | ||
| BBa_K1486029 | Nterm sfGFP + kan | Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene | ||
| BBa_K1486030 | Nterm rLuc + kan | Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene | ||
| BBa_K1486031 | Ura | CDS for Uracil (yeast selective purposes) | Confer resistance to Uracil-deprived medium | |
| BBa_K1486032 | Yeast sfGFP + Ura | Yeast sfGFP attached to the Uracil CDS | Control the expression of hog1 | |
| BBa_K1486033 | rLuc + Ura | Renilla luciferase attached to the Uracil CDS | Control the expression of hog1 | |
| BBa_K1486034 | Cterm yeast sfGFP | C terminal moiety of split the yeast sfGFP | ||
| BBa_K1486035 | Cterm yeast sfGFP + Ura | Cterm moiety of split yeast sfGFP attached to the Uracil CDS | ||
| BBa_K1486036 | Cterm rLuc + Ura | Cterm moiety of split renilla luciferase attached to the Uracil CDS | ||
| BBa_K1486037 | linker | Attaches two proteins together | ||
| BBa_K1486038 | Nterm sfGFP | N terminus moiety of split superfolder GFP | ||
| BBa_K1486039 | Cterm sfGFP | C terminus moiety of split superfolder GFP | ||
| BBa_K1486040 | Nterm sfGFP + CpxR | N terminus moiety of split sfGFP attached to CpxR | ||
| BBa_K1486041 | Cterm sfGFP + CpxR | C terminus moiety of split sfGFP attached to CpxR | ||
| BBa_K1486042 | LZip | Monomer of leucine zipper TF | ||
| BBa_K1486043 | LZip + split rLuc | Two Leucine Zipper monomers, each attached to a different split rLuc moiety | Control for split rLuc assays |
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