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Team:TU Delft-Leiden/WetLab/landmine/characterisation
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<h3> Plate Reader </h3> | <h3> Plate Reader </h3> | ||
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| - | A plate reader is a machine designed to handle samples on 6-1536 well format microtiter plates for the measuring of physical properties such as absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarisation. Concerning this module, the plate reader device was used for the measurement of fluorescence intensity generated by cells carrying the BioBricks designed to detect land mines. The protocol | + | A plate reader is a machine designed to handle samples on 6-1536 well format microtiter plates for the measuring of physical properties such as absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarisation. Concerning this module, the plate reader device was used for the measurement of fluorescence intensity generated by cells carrying the BioBricks designed to detect land mines. The final protocol developed for Plate reader analysis for this module can be found at |
<h2> LINK TO PROTOCOLS </h2> | <h2> LINK TO PROTOCOLS </h2> | ||
<h3> Results - Plate Reader </h3> | <h3> Results - Plate Reader </h3> | ||
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| + | Using the constructs BBa_K1316003 and BBa_K1316005, different concentrations of 2,4-DNT were tested: 150, 50, 16 and 0 mg/L DNT. | ||
| + | <img src="https://static.igem.org/mediawiki/2014/c/c9/TUDelft_2014_DNT_150.png" width="100%" height="100%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/4/44/TUDelft_2014_DNT_50_Tris.png" width="100%" height="100%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/d/d7/TUDelft_2014_DNT_50_Water.png" width="100%" height="100%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/9/9e/TUDelft_2014_DNT_17_Tris.png" width="100%" height="100%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/0/02/TUDelft_2014_DNT_17_Water.png" width="100%" height="100%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/d/df/TUDelft_2014_DNT_0_Tris.png" width="100%" height="100%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/a/a8/TUDelft_2014_DNT_0_Water.png" width="100%" height="100%"> | ||
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<a name="LDmicroscopy"></a> | <a name="LDmicroscopy"></a> | ||
Revision as of 15:50, 5 October 2014
Landmine Module - Characterisation
As already mentioned, the promoters found to be activated in presence of several chemical compounds that can leak from land mines (ybiJ and yqjF) were coupled to the expression of the fluorescent protein mKate2.
Assays
The different assays used to test our Land Mine BioBricks are:
Plate Reader
Microscopy
FACS
Plate Reader
A plate reader is a machine designed to handle samples on 6-1536 well format microtiter plates for the measuring of physical properties such as absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarisation. Concerning this module, the plate reader device was used for the measurement of fluorescence intensity generated by cells carrying the BioBricks designed to detect land mines. The final protocol developed for Plate reader analysis for this module can be found at
LINK TO PROTOCOLS
Results - Plate Reader
Using the constructs BBa_K1316003 and BBa_K1316005, different concentrations of 2,4-DNT were tested: 150, 50, 16 and 0 mg/L DNT.
Microscopy
FACS
Fluorescence-activated cell sorting (FACS) is a specialised type of flow cytometry
