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| | <div class="textTitle"><h4>Acknowledgements & Attributions</h4></div> | | <div class="textTitle"><h4>Acknowledgements & Attributions</h4></div> |
| | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> | | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> |
| - | <p>The UCL iGEM 2014 team would like to thank all our advisors who have assisted us throughout the project, and without whom the project would not have been possible. We would also like to thank all everyone else who has helped us realise this project, be it through invaluable advice or providing DNA, seeds, or other materials. These contributions have helped us enormously. All work on this wiki was carried out and all data collected by us unless stated otherwise. For a full list of our collaborations & acknowledgements, please visit our <a href="https://2014.igem.org/Team:UCL/Humans/Collab">collaborations</a> page.</p><br> | + | <p>The UCL iGEM 2014 team would like to thank all our advisors who have assisted us throughout the project, and without whom the project would not have been possible. We would also like to thank all everyone else who has helped us realise this project, be it through invaluable advice or providing DNA, equipment, or other materials. These contributions have helped us enormously. All work on this wiki was carried out and all data collected by us unless stated otherwise. For a full list of our collaborations & acknowledgements, please visit our <a href="https://2014.igem.org/Team:UCL/Humans/Collab">collaborations</a> page.</p><br> |
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| | </tr></table> | | </tr></table> |
| | <!---TABLE END---> | | <!---TABLE END---> |
| - | <p>Our work and achievements can be split into 6 categories, each representing the different aspects of our immense project. During the early days of our project, we identified the different subteams we would be working in, and since then a lot of members have contributed to almost all aspects of the project. To view what our project has encompassed and who has contributed accordingly, click on the tabs above. </p> | + | <p>Our work and achievements can be split into 6 categories, each representing the different aspects of our immense project. During the early days of our project, we identified the different sub-teams we would be working in, and since then a lot of members have contributed to almost all aspects of the project. To view what our project has encompassed and who has contributed accordingly, click on the tabs above. </p> |
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| | <div class="textTitle"><h4>Graphics & Design</h4></div> | | <div class="textTitle"><h4>Graphics & Design</h4></div> |
| | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> | | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> |
| - | <div class="video-wrapper">
| + | <p>We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our lab work. </p> |
| - | <iframe width="600" height="380" style="padding:1%; border:0.5% #000;" src="//www.youtube.com/embed/6UNpqpMS5vA" frameborder="0" allowfullscreen align="right"></iframe></div>
| + | |
| - | <p>We will use rapid polymer prototyping techniques to generate microfluidic chips that will allow us to test our reaction and aid in the construction of a realistic bioprocess, which can be successfully scaled-up for industrial use. As we optimise and change our bioprocess, we can also quickly design new microfluidic chips that can mimic its development on a micro-scale. For example, it is our goal to integrate multiple downstream steps, such as chromatography, in order to isolate potential useful products. Demonstrating this in a microfluidic system is less time-consuming and far more cost effective than doing so at a larger scale.</p><br> | + | |
| - | <p>For our microfluidic bioreactor, we will be using a magnetic free floating bar as our mixing system. This is an effective method of mixing at a microfluidic scale, as demonstrated in the video on the right. This video is of a microfluidic chemostat bioreactor designed by Davies et al. 2014 UCL, using a free-floating bar to mix two dyes.</p><br>
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| - | <div class="SCJMFHIGHLIGHT"><p>
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| - | <img src="https://static.igem.org/mediawiki/2014/thumb/b/b8/AutoCAD_Device.png/800px-AutoCAD_Device.png" width=32%>
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| - | <img src="https://static.igem.org/mediawiki/2014/thumb/7/71/MicrofluidicsDevice.jpg/800px-MicrofluidicsDevice.jpg" width=32% style="PADDING-LEFT: 2%" "PADDING-RIGHT: 3%">
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| - | <img src="https://static.igem.org/mediawiki/2014/1/1e/Fluidics_Chip.JPG" width=32% align="right"><br>
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| - | Above are some examples of the microfluidics devices developed by our team for use in the lab at the UCL ACBE. The devices are initially designed using AutoCAD (2D and 3D computer-aided design software), once the designs are finalised they can be 3D-printed using the facilities provided by the UCL Institute of Making and UCL ACBE; allowing our bioprocess and laboratory team to experiment and improve designs.</p></div>
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| - | <br>
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| - | <p><img src="https://static.igem.org/mediawiki/2014/4/4c/UCLAc-2014-logo.png" align="right" width="10%">
| + | |
| - | An example of one of our microfluidic devices designed on AutoCAD can be downloaded <a href="https://static.igem.org/mediawiki/2014/f/fa/UCL_iGEM_2014_Microfluidics_Device_Design.dwg.zip">here</a>. This device utilises the basic concept of mixing the cells and dyes, producing a single output stream; much alike to the <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocessing</a> concept. During the course of designing the microfluidic device, several key considerations must be taken into account: ability to withstand high pressure without leakage; materials of construction to be inert and transparent; size constraints of inlet and outlet piping; ability to accurately 3D-print the device.</p><br>
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| - | <div class="textTitle"><h4>Human Practice & Public Engagement</h4></div> | + | <div class="textTitle"><h4>Human Practices & Public Engagement</h4></div> |
| | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> | | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> |
| - | <p>Since our project involves designing a novel <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocess</a> using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.</p><br> | + | <p>We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our lab work. </p> |
| - | <!---TABLE START--->
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| - | <iframe width="600" height="380" style="padding:0.5%; border:0.5% #000;" src="//www.youtube.com/embed/0OlMfq5WT6k" frameborder="0" allowfullscreen align="left"></iframe></div></td><td>
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| - | <iframe width="600" height="380" style="padding:0.5%; border:0.5% #000;" src="//www.youtube.com/embed/buf--n4dcUs" frameborder="0" allowfullscreen align="right"></iframe></div></td></tr></table>
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| - | <img src="https://static.igem.org/mediawiki/2014/4/49/Microfluidic_set-up_iGEM.JPG" width=35% style="float:right;margin:0 0 0 10px;">
| + | |
| - | The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
| + | |
| - | <br><br>The image on the right displays the microfluidics set-up used by our iGEM team. This device and equipment is provided for by the UCL microfluidics lab.</p></div>
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| | <div class="textTitle"><h4>Modelling & General Science</h4></div> | | <div class="textTitle"><h4>Modelling & General Science</h4></div> |
| | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> | | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> |
| - | <p>Since our project involves designing a novel <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocess</a> using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.</p><br> | + | <p>We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our lab work. </p> |
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| - | <img src="https://static.igem.org/mediawiki/2014/4/49/Microfluidic_set-up_iGEM.JPG" width=35% style="float:right;margin:0 0 0 10px;">
| + | |
| - | The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
| + | |
| - | <br><br>The image on the right displays the microfluidics set-up used by our iGEM team. This device and equipment is provided for by the UCL microfluidics lab.</p></div>
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| | <div id="view6"> | | <div id="view6"> |
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| - | <div class="textTitle"><h4>Web & Communication</h4></div> | + | <div class="textTitle"><h4>Web & Communications</h4></div> |
| | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> | | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> |
| - | <p>Since our project involves designing a novel <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocess</a> using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.</p><br> | + | <p> We would like to thank our followers on Facebook, Twitter & Tumblr, as well as acknowledge the work of programmers whose open source work we utilised in the development of this Wiki. We extend our gratitude to the < a href=" https:// 2011. igem. org/ Team:DTU-Denmark/ How_to_customize_an_iGEM_wiki"> DTU- Denmark (2011)</ a> and <a href="https:// 2010.igem.org/ Team:TU_Munich/ BeyondTheLab/ WikiTutorial"> TU Munich ( 2010)< /a> teams for the guidance they provided us from their Wiki.</p> |
| - | <!---TABLE START--->
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| - | <img src="https://static.igem.org/mediawiki/2014/4/49/Microfluidic_set-up_iGEM.JPG" width=35% style="float:right;margin:0 0 0 10px;">
| + | |
| - | The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
| + | |
| - | <br><br>The image on the right displays the microfluidics set-up used by our iGEM team. This device and equipment is provided for by the UCL microfluidics lab.</p></div> | + | |
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| - | <div class="textTitle"><h4>Bioprocess Engineering</h4></div> | + | <div class="textTitle"><h4>BioBricks & BioArt</h4></div> |
| | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> | | <!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately--> |
| - | <p>Since our project involves designing a novel <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocess</a> using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.</p><br> | + | <p>We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our lab work. </p> |
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| + | |
| - | The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
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| - | <br><br>The image on the right displays the microfluidics set-up used by our iGEM team. This device and equipment is provided for by the UCL microfluidics lab.</p></div>
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