Team:PoznanBioInf

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                class='selected'       ><a href="/Http://2014hs.igem.org/Team:PoznanBioInf">Page              </a></li>
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<h2>REColi: Signal amplification and formattable bacterial memory by DNA edition.</h2>
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We have designed a synthetic digital device inspired by electronic circuits called multiplexers. Its 3-bit memory allows saving and processing binary inputs in E. coli. Induced expression of serine recombinases, capable of specific DNA editing, enables construction of biological analogues of transistors - transcriptors - and their use as elementary memory units called SR-latches. The fourth, strobe signal resets the system to its original state. We have shown that the system could efficiently store data about previous contact with inducing sugars across hundreds of generations. This memory unit offers high sensitivity in inducer detection and signal amplification, allowing cheap induction or further development and use as a trace contaminants sensor. Eight possible output combinations, reported as RGB fluorescent proteins, may also turn out to be useful for complex coexpression research.
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padding: 0px;
+
-
margin: 0px;
+
-
}
+
-
.newsItem img {
+
</td>
-
float: right;
+
-
clear: right;
+
-
width: 80px;
+
-
padding-left: 15px;
+
-
display: inline-block;
+
-
}
+
-
.newsItem .newsDate {
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<td > </td>
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font-style: italic;
+
<td width="45%">
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font-size: 14px;
+
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+
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float: right;
+
-
padding: 0px 0px 10px 15px;
+
-
}
+
-
.newsItem p, .newsItem ul, .newsItem li {
+
<p>There are a few wiki requirements teams must follow:</p>
-
padding: 0px;
+
<ul>
-
margin-left: 20px;
+
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
-
font-size: 12px;
+
<li>All pages must be created under the team’s name space.</li>
-
line-height: 1.2;
+
<li>As part of your documentation, keep the links from the menu to the left. </li>
-
}
+
<li>Do not use flash in wiki code. </li>
 +
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
 +
</ul>
 +
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
-
.newsItem p {margin-top: 10px;}
+
</td>
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</tr>
-
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-
border: 2px solid #414141;
 
-
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-
padding: 0px 15px 10px 10px;
 
-
margin-bottom: 18px;
 
-
position: relative;
 
-
height: 160px;
 
-
vertical-align: middle;
 
-
}
 
-
.annItem .annCentered {
+
<tr> <td colspan="3"  height="15px"> </td></tr>
-
display: table-cell;
+
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
-
vertical-align: middle;
+
-
height: 160px;
+
-
}
+
-
.annCentered h3 {
 
-
padding: 0px;
 
-
display: block;
 
-
margin-left: auto;
 
-
margin-right: auto;
 
-
text-align: center;
 
-
}
 
-
.annItem ul, .annItem li {padding: 0px; margin: 0px;}
+
<!--tips  -->
-
.annItem li {list-style: none; margin: 5px}
+
<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
-
 
-
 
-
</style>
 
-
</style>
 
-
 
-
 
-
 
-
 
-
 
-
<style>
 
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
 
-
</style>
 
-
 
-
<!--  here ends the section that changes the default wiki template to a white full width background -->
 
-
 
-
 
-
<!-- beginning of your page -->
 
-
 
-
<!-- menu -->
 
-
<table id="menu" width="100%"  cellspacing="0" height="135px">
 
<tr>
<tr>
-
<td bgColor="#000000"></td>
+
<td width="45%" valign="top">  
-
<td valign="bottom"  width="975px" align="center" bgColor="#000000">
+
<p>We are currently working on providing teams with some easy to use design templates.
 +
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
-
<table align="center">
+
<ul>
-
<tr height="50px">
+
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>  
+
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
-
<a href="https://2014.igem.org/WikitemplateB_home"style="text-decoration:none;color:#FFFFFF">[HOME] </a> </td>  
+
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 +
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 +
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 +
</ul>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>  
+
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a> lists. </p>
-
<a href="https://2014.igem.org/WikitemplateB_project" style="text-decoration:none;color:#FFFFFF">[PROJECT]</a> </td>
+
</td>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>  
+
<td> </td>
-
<a href="" style="text-decoration:none;color:#FFFFFF">[PARTS]</a></td>  
+
<td width="45%">  
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>  
+
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
-
<a href="" style="text-decoration:none;color:#FFFFFF">[TEAM] </a> </td>
+
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>
+
<ul>
-
<a href="" style="text-decoration:none;color:#FFFFFF">[NOTEBOOK] </a> </td>
+
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
-
 
+
<li>Be clear about what you are doing and what you plan to do.</li>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>   
+
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
-
<a href="" style="text-decoration:none;color:#FFFFFF">[SAFETY] </a></td>
+
<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
 +
<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
 +
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
 +
<li>Have lots of fun! </li>
 +
</ul>
 +
</br>
 +
</td>
</tr>
</tr>
-
 
-
 
</table>
</table>
-
 
-
 
-
 
-
 
-
</p>
 
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Retrieved from "<a href="https://2014.igem.org/Http://2014hs.igem.org/Team:UCL_Academy">https://2014.igem.org/Http://2014hs.igem.org/Team:UCL_Academy</a>"</div>
 
-
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
 
-
<div class="visualClear"></div>
 
-
</div>
 
-
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-
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-
<div class="visualClear"></div>
 
-
    <div id='footer-box' class='noprint'>
 
-
        <div id="footer">
 
-
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
 
-
 
-
 
-
  <!-- Recentchanges is not handles well DEBUG -->
 
-
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
 
-
      title='Recent changes'>Recent changes</a></li>
 
-
 
-
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Http://2014hs.igem.org/Team:UCL_Academy"
 
-
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
 
-
 
-
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Http://2014hs.igem.org/Team:UCL_Academy"
 
-
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
 
-
 
-
 
-
 
-
                <li id="t-upload"><a href="/Special:Upload"
 
-
                  title="Upload files [u]" accesskey="u">Upload file</a>
 
-
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-
                <li id="t-specialpages"><a href="/Special:SpecialPages"
 
-
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
 
-
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-
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-
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-
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-
 
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-
 
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-
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-
 
-
 
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-
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Revision as of 20:59, 4 October 2014


REColi: Signal amplification and formattable bacterial memory by DNA edition.

We have designed a synthetic digital device inspired by electronic circuits called multiplexers. Its 3-bit memory allows saving and processing binary inputs in E. coli. Induced expression of serine recombinases, capable of specific DNA editing, enables construction of biological analogues of transistors - transcriptors - and their use as elementary memory units called SR-latches. The fourth, strobe signal resets the system to its original state. We have shown that the system could efficiently store data about previous contact with inducing sugars across hundreds of generations. This memory unit offers high sensitivity in inducer detection and signal amplification, allowing cheap induction or further development and use as a trace contaminants sensor. Eight possible output combinations, reported as RGB fluorescent proteins, may also turn out to be useful for complex coexpression research.


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Requirements

Please be sure to keep these links, your audience will want to find your:

There are a few wiki requirements teams must follow:

  • All pages, images and files must be hosted on the 2014.igem.org server.
  • All pages must be created under the team’s name space.
  • As part of your documentation, keep the links from the menu to the left.
  • Do not use flash in wiki code.
  • The iGEM logo should be placed on the upper part of every page and should link to 2014.igem.org.

Visit the Wiki How To page for a complete list of requirements, tips and other useful information.

Tips

We are currently working on providing teams with some easy to use design templates.
In the meantime you can also view other team wikis for inspiration! Here are some very good examples

For a full wiki list, you can visit iGEM 2013 web sites and iGEM 2012 web sites lists.

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
  • Be clear about what you are doing and what you plan to do.
  • You have a global audience! Consider the different backgrounds that your users come from.
  • Make sure information is easy to find; nothing should be more than 3 clicks away.
  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2013 calendar
  • Have lots of fun!

Retrieved from "http://2014.igem.org/Team:PoznanBioInf"