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Team:Aix-Marseille/Safety
From 2014.igem.org
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="overall"></span> | <span class="project-tag" id="overall"></span> | ||
| - | <h1 | + | <h1 style="border-bottom-color:#f39c12">Overall</h1> |
<p>All laboratory work needs to follow safety rules and regulations. We manipulated in a CNRS laboratory on Joseph Aiguier's campus. That's why, we stuck to CNRS guidelines concerning genetically modified organism manipulation. All members of our iGEM team attended a conference on biosafety guidelines in CNRS laboratories.</p> | <p>All laboratory work needs to follow safety rules and regulations. We manipulated in a CNRS laboratory on Joseph Aiguier's campus. That's why, we stuck to CNRS guidelines concerning genetically modified organism manipulation. All members of our iGEM team attended a conference on biosafety guidelines in CNRS laboratories.</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="introduction"></span> | <span class="project-tag" id="introduction"></span> | ||
| - | <h1 | + | <h1 style="border-bottom-color:#f39c12">General introduction</h1> |
<p>The manipulation of Genetically Moified Organisms is supposed to be strictly supervised and there are specific regulations. Safety issues are separated in several areas: chemicals and hasardous products, risky devices and equipment and bacteriologic risks.</p> | <p>The manipulation of Genetically Moified Organisms is supposed to be strictly supervised and there are specific regulations. Safety issues are separated in several areas: chemicals and hasardous products, risky devices and equipment and bacteriologic risks.</p> | ||
</div> | </div> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="chemical_risks"></span> | <span class="project-tag" id="chemical_risks"></span> | ||
| - | <h1 | + | <h1 style="border-bottom-color:#f39c12">Chemical risks</h1> |
<p>We used some hazardous products which need to be vigilant.</p> | <p>We used some hazardous products which need to be vigilant.</p> | ||
<ul> | <ul> | ||
<li> | <li> | ||
| - | <h3 | + | <h3>GelRed</h3> |
<p>We used GelRed as an intercalating nucleic acid strain for agarose gel electrophoresis. It needed to be manipulated with gloves because of it chemical nature.</p> | <p>We used GelRed as an intercalating nucleic acid strain for agarose gel electrophoresis. It needed to be manipulated with gloves because of it chemical nature.</p> | ||
</li> | </li> | ||
<li> | <li> | ||
| - | <h3 | + | <h3>Diméthylsulfoxyde</h3> |
<p>DMSO was used in PCR reactions. It should be manipulated with caution because it can cause contaminants, toxins, and medicines to be absorbed through the skin.</p> | <p>DMSO was used in PCR reactions. It should be manipulated with caution because it can cause contaminants, toxins, and medicines to be absorbed through the skin.</p> | ||
</li> | </li> | ||
<li> | <li> | ||
| - | <h3 | + | <h3>Macherey Nagel MiniPrep Kit</h3> |
<p>As every kit of plasmid purification, this kit could contain toxic solvents hazardous for humans and the environment. All waste produced using this kit must be correctly treated.</p> | <p>As every kit of plasmid purification, this kit could contain toxic solvents hazardous for humans and the environment. All waste produced using this kit must be correctly treated.</p> | ||
</li> | </li> | ||
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<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="physical_risks"></span> | <span class="project-tag" id="physical_risks"></span> | ||
| - | <h1 | + | <h1 style="border-bottom-color:#f39c12">Physical risks</h1> |
<p>Some devices used during manipulation in laboratory could be risky.</p> | <p>Some devices used during manipulation in laboratory could be risky.</p> | ||
<ul> | <ul> | ||
<li> | <li> | ||
| - | <h3 | + | <h3>UV revelation machine</h3> |
<p>To reveal agarose gel and to realize gel extractions, we used an UV revelation machine. UV may cause blindness if no protection masks is worn.</p> | <p>To reveal agarose gel and to realize gel extractions, we used an UV revelation machine. UV may cause blindness if no protection masks is worn.</p> | ||
</li> | </li> | ||
<li> | <li> | ||
| - | <h3 | + | <h3>Centrifuge</h3> |
<p>Used for many protocols, the rotor in centrifuges can be dangerous, it needs to be checked before each use. It may cause death in worst case scenarios or even wreck a building and amenities.</p> | <p>Used for many protocols, the rotor in centrifuges can be dangerous, it needs to be checked before each use. It may cause death in worst case scenarios or even wreck a building and amenities.</p> | ||
</li> | </li> | ||
<li> | <li> | ||
| - | <h3 | + | <h3>-80°C Freezer</h3> |
<p>Special warm gloves are required to manipulate what is inside the freezer, can causes freeze burns to the skin.</p> | <p>Special warm gloves are required to manipulate what is inside the freezer, can causes freeze burns to the skin.</p> | ||
</li> | </li> | ||
<li> | <li> | ||
| - | <h3 | + | <h3>Water bath</h3> |
<p>Used in competent cells protocols. One have to be careful to turn it off when manipulation is over for it may cause fire if all water evaporates.</p> | <p>Used in competent cells protocols. One have to be careful to turn it off when manipulation is over for it may cause fire if all water evaporates.</p> | ||
</li> | </li> | ||
<li> | <li> | ||
| - | <h3 | + | <h3>Scalpels</h3> |
<p>Scalpels were used to slice agarose gels and more generally in of gel extraction protocols. Those who use it have to be careful not to cut themselves, and safely dispose of the blades.</p> | <p>Scalpels were used to slice agarose gels and more generally in of gel extraction protocols. Those who use it have to be careful not to cut themselves, and safely dispose of the blades.</p> | ||
</li> | </li> | ||
| Line 155: | Line 155: | ||
<div class="project-section"> | <div class="project-section"> | ||
<span class="project-tag" id="bacteriological_risks"></span> | <span class="project-tag" id="bacteriological_risks"></span> | ||
| - | <h1 | + | <h1 style="border-bottom-color:#f39c12">Bacteriological risks</h1> |
<p>All bacteria used during the project are classified risk group 1 in biosafety guidelines. We worked exclusively with Escherichia coli stains DH5α and W3110 both of which are of the K12 type, and thus not pathogenic to humans. However, there is still a small residual risk of contamination especially of eyes and the respiratory pathway.</p> | <p>All bacteria used during the project are classified risk group 1 in biosafety guidelines. We worked exclusively with Escherichia coli stains DH5α and W3110 both of which are of the K12 type, and thus not pathogenic to humans. However, there is still a small residual risk of contamination especially of eyes and the respiratory pathway.</p> | ||
<p>As the organisms used are genetically modified, cultures and microbiological waste are autoclaved after use, thus the GMOs cannot get escape from the laboratory environment. </p> | <p>As the organisms used are genetically modified, cultures and microbiological waste are autoclaved after use, thus the GMOs cannot get escape from the laboratory environment. </p> | ||
Revision as of 14:51, 4 October 2014
Safety issues
Overall
All laboratory work needs to follow safety rules and regulations. We manipulated in a CNRS laboratory on Joseph Aiguier's campus. That's why, we stuck to CNRS guidelines concerning genetically modified organism manipulation. All members of our iGEM team attended a conference on biosafety guidelines in CNRS laboratories.
General introduction
The manipulation of Genetically Moified Organisms is supposed to be strictly supervised and there are specific regulations. Safety issues are separated in several areas: chemicals and hasardous products, risky devices and equipment and bacteriologic risks.
Chemical risks
We used some hazardous products which need to be vigilant.
-
GelRed
We used GelRed as an intercalating nucleic acid strain for agarose gel electrophoresis. It needed to be manipulated with gloves because of it chemical nature.
-
Diméthylsulfoxyde
DMSO was used in PCR reactions. It should be manipulated with caution because it can cause contaminants, toxins, and medicines to be absorbed through the skin.
-
Macherey Nagel MiniPrep Kit
As every kit of plasmid purification, this kit could contain toxic solvents hazardous for humans and the environment. All waste produced using this kit must be correctly treated.
Physical risks
Some devices used during manipulation in laboratory could be risky.
-
UV revelation machine
To reveal agarose gel and to realize gel extractions, we used an UV revelation machine. UV may cause blindness if no protection masks is worn.
-
Centrifuge
Used for many protocols, the rotor in centrifuges can be dangerous, it needs to be checked before each use. It may cause death in worst case scenarios or even wreck a building and amenities.
-
-80°C Freezer
Special warm gloves are required to manipulate what is inside the freezer, can causes freeze burns to the skin.
-
Water bath
Used in competent cells protocols. One have to be careful to turn it off when manipulation is over for it may cause fire if all water evaporates.
-
Scalpels
Scalpels were used to slice agarose gels and more generally in of gel extraction protocols. Those who use it have to be careful not to cut themselves, and safely dispose of the blades.
Bacteriological risks
All bacteria used during the project are classified risk group 1 in biosafety guidelines. We worked exclusively with Escherichia coli stains DH5α and W3110 both of which are of the K12 type, and thus not pathogenic to humans. However, there is still a small residual risk of contamination especially of eyes and the respiratory pathway.
As the organisms used are genetically modified, cultures and microbiological waste are autoclaved after use, thus the GMOs cannot get escape from the laboratory environment.
