Team:ITESM-CEM/Parts
From 2014.igem.org
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- | <sub> | + | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Parts" style="color: #FFF;">Our Parts</a></sub> |
- | + | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/List" style="color: #FFF;">List of our parts</a></sub> | |
+ | </ul></td> | ||
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+ | <h2>Our Parts</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word> The main goal of our project was to establish the construct which will help us to degrade 7-ketocholesterol consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related with atherosclerosis. | ||
+ | </p><br><br> | ||
- | < | + | <h2>Cholesterol Oxidase</h2> |
- | + | <p style="text-align: justify; text-justify: inter-word> This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin. | |
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- | <h2> | + | <h2>Oxoacyl Reductase</h2> |
+ | <p style="text-align: justify; text-justify: inter-word> This enzyme was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1007 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin. | ||
+ | </p> <br><br> | ||
- | < | + | <h2>7-dehydratase</h2> |
- | </p> | + | <p style="text-align: justify; text-justify: inter-word> This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin. |
+ | </p> <br><br> | ||
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+ | <h2>Neomycin Resistance</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word> This selective marker was gotten from a plasmid for mammalian expression, its length is of 855 nucleotides,and it was isolated from pcDNA3.1(+)/myc-His A. | ||
+ | </p><br><br> | ||
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+ | <h2>BGHPA</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word> Stop coding for eucaryotic cells | ||
+ | </p><br><br> | ||
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+ | <h2>PCMV</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word> Promoter from Human Cytomegalovirus, this promoter works only on eucaryotic cells. | ||
+ | </p><br><br><br> | ||
+ | |||
+ | <br> | ||
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Revision as of 15:19, 2 October 2014
ITESM-CEM | Enzy7-K me |
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