Team:Berlin/Project

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  <a class="navmenu-brand" href="http://igem.berlin/index.php"><h1>iGEM Berlin</h1></a>
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    <li><a href="http://igem.berlin/index.php" class="active">Home</a></li>
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    <li><a href="http://igem.berlin/project.php">Project</a></li>
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    <li><a href="http://igem.berlin/team.php">Team</a></li>
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    <h1 class="cover-heading green bubble">iGEM BERLIN</h1>
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    <p class="teaser">
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      Remote-controlled E.coli bacteria?
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      That’s the aim of the interdisciplinary iGEM Team Berlin! <br />
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    </p>
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    <div id="main-button" class="hidden-xs hidden-sm hidden-md"> <a class="btn-lg" ><img src="https://static.igem.org/mediawiki/2014/9/9a/Team_Berlin_igem_arrow_down.png"> </a></div> 
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          <h2><img id="" src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Magnetic e.Coli?</h2>
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          <p>
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          The bacteria shall be optimized for the absorption and synthesis of iron in order to
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          remote-control them magnetically. Small and efficient E.coli units with various areas
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          of use shall be the result.<br><br>These cells are attractive as they offer new solutions in bio therapeutics, bio materials and bio process engineering. Furthermore, we want to use these cells as remote controlled bacteria that can be controlled by a real time computer interface.</p>
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          <a href="http://igem.berlin/project.php" class="more-informations-white hidden-xs hidden-sm hidden-md">More information</a>
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        <div id="ecoli-button" class="hidden-xs hidden-sm hidden-md"> <a class="btn-lg" ><img src="https://static.igem.org/mediawiki/2014/3/37/Team_Berlin_igem_arrow_down_white.png"> </a></div> 
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          <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Project</h2>
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          <p>In October 2014, a team of Berlin students from a variety of backgrounds are participating
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          for the first time in the in the international genetically engineered machines competition.
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          For our project, the iGEM Berlin team has decided to make E. coli bacteria susceptible to
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          magnetic fields. By knocking out the iron efflux transporter (FieF) and iron uptake
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          suppressor (FUR), we want to increase the total iron level of the cytosol. By sequestering
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          iron in a ferritin protein, iron crystals are formed and the cell is detoxified. In order
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          to create ferromagnetic of super paramagnetic crystals, we will then use an intensive high
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          through put media optimization to discover the best conditions for the formation of magnetic
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          nanoparticles in E. coli. Furthermore, we will work with other metal binding proteins like
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          metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis in
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          E. coli. Once we have discovered the best way to magnetize E. coli bacteria we will build
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          and characterize a suitable BioBrick that can be used by any research lab or iGEM team in
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          the world in order to remote control the movement of E. coli cells.</p>
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          <a href="http://igem.berlin/project.php" class="more-informations-white hidden-xs hidden-sm hidden-md">More information</a>
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          <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Team</h2>     
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          <p>The iGEM Team Berlin consists of 17 students from various universities in Berlin.
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          Represented are the Technische Universität Berlin, University of Applied Sciences Berlin,
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          Freie Universität as well as the Humboldt University. The team members come from diverse
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          disciplines. From biology, biotechnology, biochemistry, computer science, mathematics, mechanical
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          engineering to the history of science and technology, everything is represented. Just the
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          interdisciplinary nature of the team allows us to jointly develop solution strategies from
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          different perspectives. Hosted by Prof. Dr. Nediljko Budisa from the TU Berlin, our lab is
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          located at the Müller-Breslau-Straße 10, 10623 Berlin. Come by for a visit (Monday 5 pm).</p>
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          <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Safety</h2>
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          <p>
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          Our country uses a four-part „Safety Level“ rating system for laboratories. Level 4 is used for the most dangerous organisms.
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          This is equivalent to the WHO system. The Safety Level of our lab is Level 1 (low risk).
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        ©2014 iGem Berlin, Layout/Umsetzung: Valerian Blos, Marius Wessel
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<h1 >WELCOME TO iGEM Berlin 2014! </h1>
 
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<p>Hello everyone this is the preliminary wiki of iGEM Berlin. We are working on magnetic E. coli and if you would like to know why and how we do that just visit our homepage.
 
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<br>Visit http://www.igem.berlin </p>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Berlin/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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            if(url == (this.href)) {
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// my scroll to
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$("#main-button").click(function() {
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// my scroll to
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$("#ecoli-button").click(function() {
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<p>As previous iGEM teams have shown, synthesizing fully functional magnetosomes in E. coli is highly difficult as more than 60 highly regulated genes are involved. As a more feasible alternative, we simply want to synthesize magnetic nanoparticles in E. coli in order to attract cells with strong magnetic fields.
 
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Revision as of 13:55, 2 October 2014

iGEM BERLIN

Remote-controlled E.coli bacteria? That’s the aim of the interdisciplinary iGEM Team Berlin!

Magnetic e.Coli?

The bacteria shall be optimized for the absorption and synthesis of iron in order to remote-control them magnetically. Small and efficient E.coli units with various areas of use shall be the result.

These cells are attractive as they offer new solutions in bio therapeutics, bio materials and bio process engineering. Furthermore, we want to use these cells as remote controlled bacteria that can be controlled by a real time computer interface.

Project

In October 2014, a team of Berlin students from a variety of backgrounds are participating for the first time in the in the international genetically engineered machines competition. For our project, the iGEM Berlin team has decided to make E. coli bacteria susceptible to magnetic fields. By knocking out the iron efflux transporter (FieF) and iron uptake suppressor (FUR), we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. In order to create ferromagnetic of super paramagnetic crystals, we will then use an intensive high through put media optimization to discover the best conditions for the formation of magnetic nanoparticles in E. coli. Furthermore, we will work with other metal binding proteins like metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis in E. coli. Once we have discovered the best way to magnetize E. coli bacteria we will build and characterize a suitable BioBrick that can be used by any research lab or iGEM team in the world in order to remote control the movement of E. coli cells.

Team

The iGEM Team Berlin consists of 17 students from various universities in Berlin. Represented are the Technische Universität Berlin, University of Applied Sciences Berlin, Freie Universität as well as the Humboldt University. The team members come from diverse disciplines. From biology, biotechnology, biochemistry, computer science, mathematics, mechanical engineering to the history of science and technology, everything is represented. Just the interdisciplinary nature of the team allows us to jointly develop solution strategies from different perspectives. Hosted by Prof. Dr. Nediljko Budisa from the TU Berlin, our lab is located at the Müller-Breslau-Straße 10, 10623 Berlin. Come by for a visit (Monday 5 pm).

Safety

Our country uses a four-part „Safety Level“ rating system for laboratories. Level 4 is used for the most dangerous organisms. This is equivalent to the WHO system. The Safety Level of our lab is Level 1 (low risk).

Müller-Breslau-Str. 10
10623 Berlin
Phone: +49 1778800244
Mail: mail@igem.berlin
©2014 iGem Berlin, Layout/Umsetzung: Valerian Blos, Marius Wessel

Retrieved from "http://2014.igem.org/Team:Berlin/Project"