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Team:Goettingen/proLP
From 2014.igem.org
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<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA">DNA Assessment</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA">DNA Assessment</a></h4> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA# | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#rest_DNA">Restriction of DNA</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA# | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#liga_DNA">Ligation of DNA fragments</a></li> |
| - | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#seam_clone">SEAMLESS Cloning</a></li> | |
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA# | + | |
</ul> | </ul> | ||
<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | ||
| Line 30: | Line 29: | ||
<ul> | <ul> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_comp"><i>E.coli</i> competent cells</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_comp"><i>E.coli</i> competent cells</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_plas">Plasmid isolation (<i>E.coli</i>)</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_tran"><i>E.coil</i> transformation</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_tran"><i>E.coil</i> transformation</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_iso">Plasmid isolation</a></li> | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_iso">Plasmid isolation (Yeast)</a></li> |
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_tran">Yeast transformation</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_tran">Yeast transformation</a></li> | ||
</ul> | </ul> | ||
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<ul> | <ul> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_col"><i>E.coli</i> colony PCR</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y_col">Yeast colony PCR</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y_col">Yeast colony PCR</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_Scr | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_Scr">Y2H Assay with Robot</a></li> |
| - | + | ||
</ul> | </ul> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 11:46, 2 October 2014
Overview
PCR Methods
DNA Assessment
Plasmid Construction
Plasmid Transformation
- E.coli competent cells
- Plasmid isolation (E.coli)
- E.coil transformation
- Plasmid isolation (Yeast)
- Yeast transformation
