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Team:Goettingen/notebook materials
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Ampicillin 100 µg/mL<br /> | Ampicillin 100 µg/mL<br /> | ||
Gentamicin 10 µg/mL<br /> | Gentamicin 10 µg/mL<br /> | ||
| - | Kanamycin <br /><br /> | + | Kanamycin 50 µl/mL<br /> |
| + | Chloramphenicol 15 µg/mL<br /><br /> | ||
<b>Supplements</b><br /> | <b>Supplements</b><br /> | ||
| Line 31: | Line 32: | ||
<b>LB medium</b><br /> | <b>LB medium</b><br /> | ||
| + | For 1 liter:</br> | ||
10 g Tryptone <br /> | 10 g Tryptone <br /> | ||
5 g Yeast Extract<br /> | 5 g Yeast Extract<br /> | ||
| Line 37: | Line 39: | ||
15 g agar-agar <br /><br /> | 15 g agar-agar <br /><br /> | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<b>YPD medium</b><br /> | <b>YPD medium</b><br /> | ||
| + | For 1 liter:<br /> | ||
20 g Tryptone <br /> | 20 g Tryptone <br /> | ||
10 g Yeast Extract <br /> | 10 g Yeast Extract <br /> | ||
20 g Glucose <br /> | 20 g Glucose <br /> | ||
| + | <i>or</i> after autoclaving 50 mL of 40 % Glucose-solution<br /> | ||
add optional:<br /> | add optional:<br /> | ||
15 g agar-agar <br /><br /> | 15 g agar-agar <br /><br /> | ||
<b>YPAD medium</b><br /> | <b>YPAD medium</b><br /> | ||
| - | + | prepare YPD medium and add 120 mg adenine.<br /><br /> | |
| - | + | ||
| - | + | <b>50 fold ASPA solution</b><br /> | |
| - | + | For 1 liter:<br /> | |
| - | + | 297.45 g NaNO3, <br /> | |
| - | + | 26.09 g KCl, <br /> | |
| - | + | 76.21 g KH2PO4, <br /> | |
| - | + | pH 5.5<br /><br /> | |
| + | |||
| + | <b>1000 fold Trace elements solution</b><br /> | ||
| + | For 1 liter: <br />12.27 g ZnSO4, <br />11.01 g H3Bo3,<br /> 2.73 g FeSO4, <br />3.15 g MnCl2, <br />0.922 g CoCl2,<br /> 1.022 g CuSO4, <br />1.277 g Na2MoO4, <br />64.77 g Na2EDTA<br /><br /> | ||
| + | |||
| + | <b>Minimal medium for Aspergillus cultivation</b><br /> | ||
| + | For 1 liter:<br /> 25 mL Glucose 40 %, <br />1 mL Trace elements 1000x,<br /> 20 mL ASPA 50x
<br /><br /> | ||
| + | <b>50 fold TAE buffer</b><br /> | ||
| + | For 1 liter: <br />242 g Tris,<br />100 mL EDTA (0.5 M; pH 8.0) <br /> dissolve in 60 % of final volume, <br />add 57.1 mL acetic acid (100 %) <br />and fill up to 1 liter.
| ||
| + | <br /><br /> | ||
<b>SC dropout medium </b><br /> | <b>SC dropout medium </b><br /> | ||
Revision as of 10:31, 2 October 2014
Materials
Antibiotics
Ampicillin 100 µg/mL
Gentamicin 10 µg/mL
Kanamycin 50 µl/mL
Chloramphenicol 15 µg/mL
Supplements
Tryptophane 50 mg/l
Histidine 20 mg/L
Leucine 100 mg/L
Uracile 20 mg/L
Adenine 100 mg/L
3-50 mM 3-amino-1,2,4-triazole (3-AT)
LB medium
For 1 liter:
10 g Tryptone
5 g Yeast Extract
10 g NaCl
add optional:
15 g agar-agar
YPD medium
For 1 liter:
20 g Tryptone
10 g Yeast Extract
20 g Glucose
or after autoclaving 50 mL of 40 % Glucose-solution
add optional:
15 g agar-agar
YPAD medium
prepare YPD medium and add 120 mg adenine.
50 fold ASPA solution
For 1 liter:
297.45 g NaNO3,
26.09 g KCl,
76.21 g KH2PO4,
pH 5.5
1000 fold Trace elements solution
For 1 liter:
12.27 g ZnSO4,
11.01 g H3Bo3,
2.73 g FeSO4,
3.15 g MnCl2,
0.922 g CoCl2,
1.022 g CuSO4,
1.277 g Na2MoO4,
64.77 g Na2EDTA
Minimal medium for Aspergillus cultivation
For 1 liter:
25 mL Glucose 40 %,
1 mL Trace elements 1000x,
20 mL ASPA 50x
50 fold TAE buffer
For 1 liter:
242 g Tris,
100 mL EDTA (0.5 M; pH 8.0)
dissolve in 60 % of final volume,
add 57.1 mL acetic acid (100 %)
and fill up to 1 liter.
SC dropout medium
6.7 g Yeast Nitrogen Base (without amino acids)
1,46 g Amino acid mixture (-Leu/-Trp/-His/-Ura)
Adjust pH to 5.6
Autoclave 15 min only at 121°C
Add 50 mL sterile Glucose (40%), possible to add 3-50 mL 3-AT
SOB medium
20 g tryptone
5 g Yeast extract
0,584 g NaCl
0,186 g KCl
ad dH2O to 1000 mL
2,032 g MgCl2
2,064 g MgSO4
pH with NaOH to 7.0
