Team:USyd-Australia/Notebook

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Revision as of 08:37, 2 October 2014

iGEM_Link


Primers

Notebook

Date

Day

Activity

Who?
15/5 Thursday Preparation of common solutions:
  • LB
  • Stock Streptomycin (Sm) 200mg/mL, Chloramphenicol (Cm) 25 (mg/mL)
  • Sm200 and Cm25 plates
Callum and Jeanne
16/5 Friday Preparation of common solutions:
  • Stock Kanamycin (Km) 50 (mg/ml)
  • Km50 plates
Abi and Tom
22/5 Thursday Preparation of common solutions:
  • 2M NaOH
  • Stock Trimethoprim (Tm) 50mg/mL
Restriction digest:
  • pK18 digested with HindIII, EcoRI, BamHI
Agarose Gel Electrophoresis:
  • pK18 digests and undigested DNA
  • Post-stained with GelRed
  • Results...?
Callum and Jeanne
23/5 Friday Growing up gene source E. coli
  • JM109 containing pK18, for gene ____, on LB-Km
  • _____ containing R388, for gene ____, on LB-Tm
  • JM109 containing pUS44, for Pc-AttI1, on LB-Sm
  • Transform pUS41 by heat shock into Top10, grow on Sm (in hindsight, Top10 already has Sm resistance, so this was not valid)
One Loopful of each culture from glycerol stocks was streaked onto appropriate selective media under aseptic conditions. All plates were incubated at 30 degrees over the weekend. 		Abi and Tom

With thanks to:

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