Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Aug

From 2014.igem.org

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</ul>
</ul>
<ul>
<ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> of <i>Fum A</i> with electrocompotetent cells</li>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> of <i>pSB1C3_T7_Fum A</i> with electrocompotetent cells</li>
</ul>
</ul>
<ul>
<ul>
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<li>Backbone (digested with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>Spe</i>I</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Pst</i>I</a>)</li>
<li>Backbone (digested with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>Spe</i>I</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Pst</i>I</a>)</li>
<ul>
<ul>
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<li><a href="http://parts.igem.org/Part:BBa_J23101" target="_blank">BBa_J23101</a></li></li>
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<li><a href="http://parts.igem.org/Part:BBa_J23101" target="_blank">BBa_J23101</a></li>
<li><a href="http://parts.igem.org/Part:BBa_J23102" target="_blank">BBa_J23102</a></li>
<li><a href="http://parts.igem.org/Part:BBa_J23102" target="_blank">BBa_J23102</a></li>
<li><a href="http://parts.igem.org/Part:BBa_J23104" target="_blank">BBa_J23104</a></li>
<li><a href="http://parts.igem.org/Part:BBa_J23104" target="_blank">BBa_J23104</a></li>
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</ul>
</ul>
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<ul>
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<li><b><i>dcuB</i></b></li>
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<ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> of RedET plasmid with electrocompotetent cells</li>
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</ul>
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<ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> of <i>pR6K</i>-cassette for the deletion of <i>dcuB</i> with electrocompotetent cells</li>
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</ul>
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</ul>
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<ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> to verify the deletion of <i>dcuB</i> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#dcuB_del_kon1" target="_blank">dcuB_del_kon1</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#dcuB_del_kon2" target="_blank">dcuB_del_kon2</a>)
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            </li>
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<ul>
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<li>Annealing temperature: 55 °C</li>
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<li>Bands not as expected (~2390 bp)</li>
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</ul>
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</ul>
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<ul>
<ul>
<li><b><i>ccm</i></b></li>
<li><b><i>ccm</i></b></li>
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</ul>
</ul>
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</ul>
</ul>
</ul>
<ul>
<ul>
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</ul>
</ul>
</ul>
</ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a>of <i>GSU 3274</i> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_fwd" target="_blank">pccH_fwd</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_rev" target="_blank">pccH_rev</a>)</li>
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<ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a>of <i>GSU 3274 </i> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_fwd" target="_blank">pccH_fwd</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_rev" target="_blank">pccH_rev</a>)</li>
<ul>
<ul>
<li>Annealing temperature: 55 &deg;C</li>
<li>Annealing temperature: 55 &deg;C</li>
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</ul>
</ul>
</ul>
</ul>
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</ul>
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<ul>
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<li><b><i>Fum A</i></b></li>
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<ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> of <a href="http://parts.igem.org/Part:BBa_J23102" target="_blank">BBa_J23102</a>_ <i>Fum A </i> with electrocompotetent cells</li>
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</ul>
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<ul>
 +
<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VF-Primer" target="_blank">VF-Primer</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VR-Primer" target="_blank">VR-Primer</a>)
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            </li>
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<ul>
 +
<li>Annealing temperature: 55 °C</li>
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<li>Band as expected (~2200 bp)</li>
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</ul>
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</ul>
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<ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <a href="http://parts.igem.org/Part:BBa_J23102" target="_blank">BBa_J23102</a>_ <i>Fum A </i> </li>
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</ul>

Revision as of 21:25, 1 October 2014


August

Week 1     08/04 - 08/10
Week 1     08/04 - 08/10
Week 2     08/11 - 08/17
Week 2     08/11 - 08/17
  • pR6K
    • The pR6K-cassette for the dcuB deletion was purified out of the gel
  • dcuB
    • Transformation of pR6K-cassette for the deletion of dcuB with electrocompotetent cells

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