Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Aug

From 2014.igem.org

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<li><b><i>pR6K</i></b></li>
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<ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> of <i>pR6K</i>-cassette</li>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>pR6K</i>-cassette and <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#DNAPurificationbyCentrifugation" target="_blank">Purification</a> out of the gel</li> </li>
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<li><b>Connection of the <i>pR6K</i>-cassette and <i>oprF</i></b></li>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> to connect the <i>pR6K</i>-cassette and <i>oprF</i>
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<li>Bands as expected (~3300 bp)</li>
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</ul>
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<li>PCR product was <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#DNAPurificationbyCentrifugation" target="_blank">purified</a> out of the gel</li>
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Revision as of 16:09, 1 October 2014


August

Week 1     08/04 - 08/10
Week 1     08/04 - 08/10

Retrieved from "http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Aug"