Team:Hong Kong HKUST/wetlab

From 2014.igem.org

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<h2>Wetlab</h2>
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<h2>Protocol</h2>
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<p>The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely:
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<p>We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique
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(1) (Detecting Module) ComD & ComE, (2) (Detecting Module) P<sub><i>comCDE</i></sub>, (3) (Regulation Module) ComX & ComW, and
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  can be found here!
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(4) (Regulation Module) combox. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively
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and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows.
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The BioBrick, researcher, environmental and university safety issues can be found in Safety page. In terms of project process,
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some experiments could not be completed due to time constrains and they are outlined in Future Work page.
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<h4> Protocol</h4>
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<h4> Mutagenesis</h4>
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,  
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,  
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visibly touching the cheekbone below the eye. </p>
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<h4> Western Blot </h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
visibly touching the cheekbone below the eye. </p>
visibly touching the cheekbone below the eye. </p>
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<h4> LB agar and LB plates </h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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visibly touching the cheekbone below the eye. </p>
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<h4> Notebook </h4>
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<h4> Ligation </h4>
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,  
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,  
visibly touching the cheekbone below the eye. </p>
visibly touching the cheekbone below the eye. </p>
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<h4> Safety </h4>
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<h4> Ethanol Precipitation</h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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visibly touching the cheekbone below the eye. </p>
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<h4> Pbad protocols </h4>
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,  
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,  
 +
visibly touching the cheekbone below the eye. </p>
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</div>
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</td>
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<td class= "start_up_link_button">
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<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">
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<h4> Gibson Assembly </h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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visibly touching the cheekbone below the eye. </p>
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</div>
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</td>
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<td class= "start_up_link_button">
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<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">
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<h4> Polymerase Chain Reaction (PCR)</h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
visibly touching the cheekbone below the eye. </p>
visibly touching the cheekbone below the eye. </p>
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Revision as of 03:39, 29 September 2014

Protocol

We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique can be found here!

Mutagenesis

Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth, visibly touching the cheekbone below the eye.

Western Blot

Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth, visibly touching the cheekbone below the eye.

LB agar and LB plates

Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth, visibly touching the cheekbone below the eye.

Ligation

Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth, visibly touching the cheekbone below the eye.

Ethanol Precipitation

Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth, visibly touching the cheekbone below the eye.

Pbad protocols

Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth, visibly touching the cheekbone below the eye.

Gibson Assembly

Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth, visibly touching the cheekbone below the eye.

Polymerase Chain Reaction (PCR)

Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth, visibly touching the cheekbone below the eye.

Home

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