document.getElementById("tab1").innerHTML = '<h2>Assembly Plan</h2><img class="schedule" src="https://static.igem.org/mediawiki/2014/6/6e/Uppsala2014_TheYensystem.png"</img><h2> Communication using quorum sensing</h2><p>Quorum sensing (QS) is the communication tool of bacteria. It is most often done via excretion and detection of small organic molecules or proteins. This can cause different behaviors of bacteria to be density triggered, i.e. only appear when there are enough of the bacteria present in one area. Quorum sensing can control a lot of different things, from biofilm formation to bioluminescence. The most commonly known and most explored QS system is the Lux system, which was originally isolated from Vibrio fischeri, where it controls the production of a luciferase that produces a yellow light.</p><h2> Using the pathogens own quorum sensing system to detect and track its source</h2><p>When we decided that we wanted to fight pathogens it quickly became obvious that we needed a way to detect and track them. After doing some research, we decided that this was best done by hijacking the pathogens own quorum sensing. Naturally the first thought that came to our mind was to use the Lux system from Vibro Fischeri. However this was done in 2008 by Heidelberg and it also didn’t give us the specificity that we wanted our system to have. So we decided to dig deeper and see if we could find a more unique quorum sensing system. Among some potential candidates was Y. enterocolitica, which used a quorum sensing system called the Yen system.</p><h2>The Yen system </h2><p>It turned out that Y. enterocolitica had a homologous qourum sensing system to the famous Lux system, the Yen system. From this system we chose to steal two parts. A recognition region, fused togheter with a promoter, called the yenbox and a transcription factor, YenR, that can recognize and interact with the yenbox. When active YenR binds to the yenbox, inducing the expression rate of the promoter fused to the yenbox. Later, when Y. enterocolitica becomes present, Yersinias signaling molecules, a pheromone named 3-oxo-hexanoyl homoserine lactone (OHHL), will start flowing into our system, interacting with YenR. When binding occurs between OHHL and YenR, YenR will lose its shape and thereby its ability to interact with the yenbox. The induction will then be lost and the expression rate will return to its base level. <br></br>By BioBricking and characterising this system, we wish to make it possible for ourself and others to get Yersinia density triggered response. Our goal with this system is to use it to control our two other systems, the Targeting and the Killing system. </p>';