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Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Jun
From 2014.igem.org
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<div class="content" style="margin-right:10%; margin-left:10%"> | <div class="content" style="margin-right:10%; margin-left:10%"> | ||
<ul> | <ul> | ||
| - | <li><b>Initial cultivation experiments with neutral red </b> | + | <li><b>Initial cultivation experiments with neutral red </b></li> |
| + | <ul> | ||
| + | <li> This week we wanted to test the properties of neutral red during a cultivation on a small scale </li> | ||
<ul> | <ul> | ||
| - | <li> Cultivation of the <i>E. coli</i> strain with porines (attempt 1) and wildtyp as control (attempt 2) in LB-medium with 100 µM neutral red </li> | + | <li> Cultivation of the <i>E. coli</i> strain with porines (attempt 1) and wildtyp as control (attempt 2)in LB-medium with 100 µM neutral red </li> |
<li> pH was set up at 8.75 </li> | <li> pH was set up at 8.75 </li> | ||
<li> Results </li> | <li> Results </li> | ||
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<li> Attempt 1 turned red (pH = 7.495)</li> | <li> Attempt 1 turned red (pH = 7.495)</li> | ||
<li> Attempt 2 remained yellow </li> | <li> Attempt 2 remained yellow </li> | ||
| + | </ul> | ||
</ul> | </ul> | ||
</ul> | </ul> | ||
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<ul> | <ul> | ||
| - | <li><b> Reduction of neutral red </b> | + | <li><b> Reduction and Oxidation of neutral red </b> |
| + | <ul> | ||
| + | <li> This week we checked the properties of neutral red under oxidizing and reducing conditions by adding chemical agents </li> | ||
<ul> | <ul> | ||
<li> Reducing agent: sodium dithionite </li> | <li> Reducing agent: sodium dithionite </li> | ||
Revision as of 17:38, 26 September 2014
June |
 |
- Initial cultivation experiments with neutral red
- This week we wanted to test the properties of neutral red during a cultivation on a small scale
- Cultivation of the E. coli strain with porines (attempt 1) and wildtyp as control (attempt 2)in LB-medium with 100 µM neutral red
- pH was set up at 8.75
- Results
- Attempt 1 turned red (pH = 7.495)
- Attempt 2 remained yellow
- Reduction and Oxidation of neutral red
- This week we checked the properties of neutral red under oxidizing and reducing conditions by adding chemical agents
- Reducing agent: sodium dithionite
- Red solution got reduced and turned colorless
- Got oxidised by air again afterwards
- Oxidizing agent: hydrogen peroxide
- Red solution stayed red
- Yellow solution stayed yellow
- Conclusion: the color of the solution depends on the pH
