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Team:UFAM Brazil/7-8-2014
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Revision as of 20:41, 25 September 2014
07/08/2014 | ||
Unfortunately, we couldn’t transform the diluted DNA from the 2013 DNA kit. Today ,instead of trying to transform using JM110 by washing it with sorbitol 1M or use a commercial cell. We used Escherichia coli DH5α chemically competent cell to transform biobrick BBa_E0840. We also transformed Escherichia coli DH5α with pUC72 having approximately 1.000ng/ul and 1ng/ul, because we wanted to find out if the reason why the diluted DNA didn’t work was dut to its low concentration. | ||
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