Team:Evry/Notebook/Sensing/PCBs/07-28-2014

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<u>Sensor construction bphR1/bphR2:</u>
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<b><u>Sensor construction bphR2/PbphR1:</u></b>
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<li>Plate 20µL on LB-agar-Cam (25 mL LB-Agar + 25µL Cam)
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<li>Plate 20µL from the culture of  <a href="https://2014.igem.org/Team:Evry/Notebook/Sensing/PCBs/07-27-2014">27th july</a> on LB-agar-Cam (25 mL LB-Agar + 25µL Chloramphenicol)
<li>Incubation of plate at 37°C overnight
<li>Incubation of plate at 37°C overnight
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<li>A glycerol stock was made with 750ml of culture and 750ml of glycerol 50%
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<li>A glycerol stock was made with 750ml of culture and 750ml of glycerol 50% and stored at -80°C
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Latest revision as of 14:18, 24 September 2014

Picture

Sensor construction bphR2/PbphR1:

  1. Plate 20µL from the culture of 27th july on LB-agar-Cam (25 mL LB-Agar + 25µL Chloramphenicol)
  2. Incubation of plate at 37°C overnight
  3. A glycerol stock was made with 750ml of culture and 750ml of glycerol 50% and stored at -80°C

Jul 28