Team:Evry/Notebook/Sensing/PCBs/07-27-2014
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- | <u>Sensor construction | + | <br/> |
+ | <b><u>Sensor construction bphR2/PbphR1:</u></b> | ||
<ol> | <ol> | ||
- | <li>Culturing | + | <li>Culturing of one colony bphR1 promoter <a href="http://parts.igem.org/Part:BBa_K1155001">(BBa_K1155001)</a>, received from Paris-Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam) |
- | <li>Incubation at 37°C overnight | + | <li>Incubation at 37°C overnight, 200rpm |
</ol> | </ol> | ||
Latest revision as of 14:17, 24 September 2014
Sensor construction bphR2/PbphR1:
- Culturing of one colony bphR1 promoter (BBa_K1155001), received from Paris-Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
- Incubation at 37°C overnight, 200rpm