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Team:Gothenburg/Modeling
From 2014.igem.org
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<h3>Introduction</h3> | <h3>Introduction</h3> | ||
<p>Our project intends to create a generation counter in Saccharomyces cerevisiae. The yeast cell would express fluorescent proteins of different colors according to the number of daughter cells spawn. Nowadays, the replicative age of a yeast cell is determined by counting the budding scars with the help of a microscope. This process is time-consuming and not automated. Once our idea is implemented, it would be possible to use a flow cytometric device to sort the cells according to their color and, consequently, generation number. To achieve that, we are building genetic circuits with a logical AND gate ensuring generation specific expression of different fluorescent proteins. | <p>Our project intends to create a generation counter in Saccharomyces cerevisiae. The yeast cell would express fluorescent proteins of different colors according to the number of daughter cells spawn. Nowadays, the replicative age of a yeast cell is determined by counting the budding scars with the help of a microscope. This process is time-consuming and not automated. Once our idea is implemented, it would be possible to use a flow cytometric device to sort the cells according to their color and, consequently, generation number. To achieve that, we are building genetic circuits with a logical AND gate ensuring generation specific expression of different fluorescent proteins. | ||
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| + | <div class="imgRight" style="padding-left: 200px"> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/8/88/Team_Gothenburg_And_Gate.png" | ||
| + | width="349px" height="113px" style="padding-left:80px"/> | ||
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| + | <p>Figure 1. Schematic representation of the logical AND gate with the input | ||
| + | and output signals. | ||
| + | </p> | ||
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Revision as of 16:20, 23 September 2014
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IntroductionOur project intends to create a generation counter in Saccharomyces cerevisiae. The yeast cell would express fluorescent proteins of different colors according to the number of daughter cells spawn. Nowadays, the replicative age of a yeast cell is determined by counting the budding scars with the help of a microscope. This process is time-consuming and not automated. Once our idea is implemented, it would be possible to use a flow cytometric device to sort the cells according to their color and, consequently, generation number. To achieve that, we are building genetic circuits with a logical AND gate ensuring generation specific expression of different fluorescent proteins. 
Figure 1. Schematic representation of the logical AND gate with the input and output signals. |
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