Team:UFMG Brazil

From 2014.igem.org

(Difference between revisions)
m
Line 1: Line 1:
-
{{tiago_test}}
+
<html>
 +
<style type="text/css">
 +
body {
 +
background-image: url(https://static.igem.org/mediawiki/2014/f/ff/WikiBG.png);
 +
text-align: left;
 +
}
 +
#docs-internal-guid-7635cd64-d63a-36ce-4e21-9371354a99a8 p {
 +
font-weight: bold;
 +
}
 +
#docs-internal-guid-7635cd64-d63a-36ce-4e21-9371354a99a8 p {
 +
font-weight: bold;
 +
}
 +
#docs-internal-guid-7635cd64-d63a-36ce-4e21-9371354a99a8 p {
 +
text-align: right;
 +
}
 +
#docs-internal-guid-7635cd64-d63a-36ce-4e21-9371354a99a8 p {
 +
text-align: justify;
 +
}
 +
</style>
-
<html xmlns="http://www.w3.org/1999/xhtml">
+
<!--main content -->
-
<head>
+
<table width="70%" height="829" align="center">
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
-
<title>Igem UFMG Brazil</title>
+
-
<meta name="keywords" content="" />
+
-
<meta name="description" content="" />
+
-
<link href="http://fonts.googleapis.com/css?family=Source+Sans+Pro:200,300,400,600,700,900" rel="stylesheet" />
+
-
<link href="default.css" rel="stylesheet" type="text/css" media="all" />
+
-
<link href="fonts.css" rel="stylesheet" type="text/css" media="all" />
+
-
<!--[if IE 6]><link href="default_ie6.css" rel="stylesheet" type="text/css" /><![endif]-->
+
<!--welcome box -->
 +
<tr>
 +
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FFFFFF>
 +
<h1 >WIKI UNDER CONSTRUCTION</h1>
 +
<p>Hello guys!! This year our team are proposing a project for another serious disease that thousands of people are
 +
  affected every year in the whole world:<br>
 +
  We intend to help in the colon cancer diagnosis!
 +
<br>
 +
<a href="https://2014.igem.org/Team:UFMG_Brazil"style="color:#000000"></a></p>
 +
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UFMG_Brazil&action=edit"style="color:#000000"> Click here  to edit this page!</a> </p>
 +
</td>
 +
</tr>
-
</head>
+
<tr> <td colspan="3" height="5px"> </td></tr>
-
<body>
+
<!-- end welcome box -->
-
<div id="header-wrapper">
+
<tr>  
-
<div id="header" class="container">
+
-
<div id="logo">
+
-
<img src="https://static.igem.org/mediawiki/2014/7/77/Logooverlay.png" alt="Logo"/>
+
-
<h1><a href="#">The Colon Yeast</a></h1>
+
-
<!--<span>Template Design by <a href="http://www.freecsstemplates.org/" rel="nofollow">FreeCSSTemplates.org</a></span> --></div>
+
-
<div id="menu">
+
-
<ul>
+
-
<li class="current_page_item"><a href="#" accesskey="1" title="">Home</a></li>
+
-
<li><a href="#" accesskey="2" title="">Safety</a></li>
+
-
<li><a href="#" accesskey="3" title="">Project</a></li>
+
-
<li><a href="#" accesskey="5" title="">Human Practices</a></li>
+
-
<li><a href="#" accesskey="5" title="">About Us</a></li>
+
-
</ul>
+
-
</div>
+
-
</div>
+
-
</div>
+
 +
<!--navigation menu -->
 +
<td align="center" colspan="3">
-
<div id="wrapper3">
+
<table  width="100%">
-
<div id="portfolio" class="container">
+
<tr heigth="15px"></tr>
-
<div class="title">
+
<tr heigth="75px">  
-
<h2>Team</h2>
+
-
<span class="byline"><a href='#' class="button button-big"> Meet us all! </a> </div>
+
-
<div class="column1">
+
-
<div class="box"><img src="https://static.igem.org/mediawiki/2014/4/41/Liza.jpg" alt="" class="image image-full" />
+
-
<h3>Liza Felicori</h3>
+
-
<p>Fermentum nibh augue praesent a lacus at urna congue rutrum.</p>
+
-
<a href="#" class="button button-small">Know More</a> </div>
+
-
</div>
+
-
<div class="column2">
+
-
<div class="box"><img src="images/scr02.jpg" alt="" class="image image-full" />
+
-
<h3>Praesent scelerisque</h3>
+
-
<p>Vivamus fermentum nibh in augue praesent urna congue rutrum.</p>
+
-
<a href="#" class="button button-small">Know More</a> </div>
+
-
</div>
+
-
<div class="column3">
+
-
<div class="box"><img src="https://static.igem.org/mediawiki/2014/3/35/Clara.jpg" alt="" class="image image-full" />
+
-
<h3>Clara Guerra</h3>
+
-
<p>Vivamus fermentum nibh in augue praesent urna congue rutrum.</p>
+
-
<a href="#" class="button button-small">Know More</a> </div>
+
-
</div>
+
-
<div class="column4">
+
-
<div class="box"><img src="https://static.igem.org/mediawiki/2014/9/93/Rodrigo.jpg" alt="" class="image image-full" />
+
-
<h3>Rodrigo</h3>
+
-
<p>Rutrum fermentum nibh in augue praesent urna congue rutrum.</p>
+
-
<a href="#" class="button button-small">Know More</a> </div>
+
-
</div>
+
-
</div>
+
 +
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 +
<a href="https://2014.igem.org/Team:UFMG_Brazil"style="color:#000000">Home </a> </td>
-
</div>
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-
<div id="wrapper1">
+
<a href="https://2014.igem.org/Team:UFMG_Brazil/Team"style="color:#000000"> Team </a> </td>
-
<div id="welcome" class="container">
+
 
-
<div class="title">
+
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
-
<h2>Welcome to our wiki</h2>
+
<a href="https://igem.org/Team.cgi?year=2014&team_name=UFMG_Brazil"style="color:#000000"> Official Team Profile </a></td>
-
<span class="byline">How to stop a war before it started?</span> </div>
+
 
-
<div class="content">
+
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-
<p>Colorectal cancer includes tumors affecting a segment of the large intestine (colon) and rectum. It is treatable and, in most cases, curable when detected early, before it hasn't spread to other organs. However, the diagnosis requires a biopsy, made ​​by handset introduced via rectum. Noticeably, this procedure is highly invasive and often inhibits patients to submit to this practice, hampering the discovery of cancer at an early stage and thereby reducing the chances of effective treatment and cure. On our institution, a modified probiotic strain of Saccharomyces cerevisiae was isolated from local beverage. Our objective is to introduce this strain as a new probiotic chassis on iGEM, and also to develop a gene circuit that allows it to detect one or more stool biomarkers for colorectal cancer on the intestine and report with a color change on the feces. We chose L-DNA (long DNA molecules) as biomarker, and designed a chimeric protein using TALE DNA-Binding Domains and a split version of mCherry. This protein can detect certain repetitive sequences on DNA and emit red fluorescence, which when measured serve as proxy for the DNA size. Since RFP emission falls on the visible spectrum, it can also be used for staining the feces, allowing for a patient consuming the probiotic to become aware of the possibility of the developing problem just by checking on the color of their stool. </p>
+
<a href="https://2014.igem.org/Team:UFMG_Brazil/Project"style="color:#000000"> Project</a></td>
-
<a href="#" class="button">Know More</a> </div>
+
 
-
</div>
+
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
-
<div id="wrapper4">
+
<a href="https://2014.igem.org/Team:UFMG_Brazil/Parts"style="color:#000000"> Parts</a></td>
-
<div id="footer" class="container">
+
 
-
<div>
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-
<header class="title">
+
<a href="https://2014.igem.org/Team:UFMG_Brazil/Modeling"style="color:#000000"> Modeling</a></td>
-
<h2>Get in touch</h2>
+
 
-
<span class="byline">Phasellus nec erat sit amet nibh pellentesque congue</span> </header>
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
-
<ul class="contact">
+
<a href="https://2014.igem.org/Team:UFMG_Brazil/Notebook"style="color:#000000"> Notebook</a></td>
-
<li><a href="#" class="icon icon-twitter"><span>Twitter</span></a></li>
+
 
-
<li><a href="#" class="icon icon-facebook"><span></span></a></li>
+
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-
<li><a href="#" class="icon icon-tumblr"><span>Google+</span></a></li>
+
<a href="https://2014.igem.org/Team:UFMG_Brazil/Safety"style=" color:#000000"> Safety </a></td>
-
+
 
-
</ul>
+
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-
</div>
+
<a href="https://2014.igem.org/Team:UFMG_Brazil/Attributions"style="color:#000000"> Attributions </a></td>
-
</div>
+
 
-
</div>
+
 
-
<div id="footer">
+
<td align ="center"><a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/b/b4/Igem_rodando.gif" width="82" height="65"></a></td>
-
<p></a></p>
+
</tr>
-
</div>
+
</table>
-
</body>
+
 
-
</html>
+
<td width="2%" align ="center"><a href="https://2014.igem.org/Main_Page">
 +
 
 +
<!--end navigation menu -->
 +
</tr>
 +
 
 +
 
 +
</tr>
 +
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
 
 +
 
 +
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
 +
<tr> <td colspan="3"  height="5px"> </td></tr>
 +
 
 +
 
 +
<!--requirements section -->
 +
<tr><td colspan="3"> <h3><strong id="docs-internal-guid-7635cd64-d633-19e2-7369-0a1a982a08ff">The Colonyeast – Project Description</strong></h3>
 +
 
 +
 
 +
<tr> <td colspan="3"  height="15px"><strong id="docs-internal-guid-7635cd64-d63a-36ce-4e21-9371354a99a8">
 +
<p dir="ltr">Colorectal cancer includes tumors affecting a segment of the large intestine (colon) and rectum. It is treatable and, in most cases, curable when detected early, before it hasn&rsquo;t spread to other organs. However, the diagnosis requires a biopsy, made ​​by handset introduced via rectum. Noticeably, this procedure is highly invasive and often inhibits patients to submit to this practice, hampering the discovery of cancer at an early stage and thereby reducing the chances of effective treatment and cure.</p>
 +
<p dir="ltr">On our institution, a modified probiotic strain of Saccharomyces cerevisiae was isolated from local beverage. Our objective is to introduce this strain as a new probiotic chassis on iGEM, and also to develop a gene circuit that allows it to detect one or more stool biomarkers for colorectal cancer on the intestine and report with a color change on the feces. We chose L-DNA (long DNA molecules) as biomarker, and designed a chimeric protein using TALE DNA-Binding Domains and a split version of mCherry. This protein can detect certain repetitive sequences on DNA and emit red fluorescence, which when measured serve as proxy for the DNA size. Since RFP emission falls on the visible spectrum, it can also be used for staining the feces, allowing for a patient consuming the probiotic to become aware of the possibility of the developing problem just by checking on the color of their stool.</p>
 +
</strong><br></td></tr>
 +
<tr>
 +
 
 +
 
 +
<!--tips  -->
 +
<tr><td colspan="3" > <h3>   </h3>
 +
</tr>
 +
</table>

Revision as of 05:58, 22 September 2014

WIKI UNDER CONSTRUCTION

Hello guys!! This year our team are proposing a project for another serious disease that thousands of people are affected every year in the whole world:
We intend to help in the colon cancer diagnosis!

Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

The Colonyeast – Project Description

Colorectal cancer includes tumors affecting a segment of the large intestine (colon) and rectum. It is treatable and, in most cases, curable when detected early, before it hasn’t spread to other organs. However, the diagnosis requires a biopsy, made ​​by handset introduced via rectum. Noticeably, this procedure is highly invasive and often inhibits patients to submit to this practice, hampering the discovery of cancer at an early stage and thereby reducing the chances of effective treatment and cure.

On our institution, a modified probiotic strain of Saccharomyces cerevisiae was isolated from local beverage. Our objective is to introduce this strain as a new probiotic chassis on iGEM, and also to develop a gene circuit that allows it to detect one or more stool biomarkers for colorectal cancer on the intestine and report with a color change on the feces. We chose L-DNA (long DNA molecules) as biomarker, and designed a chimeric protein using TALE DNA-Binding Domains and a split version of mCherry. This protein can detect certain repetitive sequences on DNA and emit red fluorescence, which when measured serve as proxy for the DNA size. Since RFP emission falls on the visible spectrum, it can also be used for staining the feces, allowing for a patient consuming the probiotic to become aware of the possibility of the developing problem just by checking on the color of their stool.


Retrieved from "http://2014.igem.org/Team:UFMG_Brazil"