Team:Evry/Interlab Study/09-18-2014

From 2014.igem.org

(Difference between revisions)
Line 23: Line 23:
<br>
<br>
<br/>
<br/>
 +
 +
 +
 +
 +
 +
Line 31: Line 37:
<i>PSB1C3+E0240+J23101</i>: <br>  
<i>PSB1C3+E0240+J23101</i>: <br>  
<ul>
<ul>
-
<li>Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br>
+
<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
</ul>
</ul>
Line 39: Line 45:
<i>PSB1C3+E0240+J23102</i>: <br>  
<i>PSB1C3+E0240+J23102</i>: <br>  
-
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
+
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
<br>
<br>
<br>
<br>
<i>PSB1C3+E0240+J23104</i>: <br>  
<i>PSB1C3+E0240+J23104</i>: <br>  
-
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
+
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
<br>
<br>
<br>
<br>
Line 64: Line 70:
<i>PSB1C3+E0240+J23115</i>: <br>  
<i>PSB1C3+E0240+J23115</i>: <br>  
-
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br>
+
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
<br>
<br>
<br>
<br>
<i>PSB1C3+E0240+J23116</i>: <br>  
<i>PSB1C3+E0240+J23116</i>: <br>  
-
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br>
+
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
<br>
<br>
<br>
<br>
<i>PSB1C3+E0240+J23117</i>: <br>  
<i>PSB1C3+E0240+J23117</i>: <br>  
-
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br>
+
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
<br>
<br>

Revision as of 20:05, 19 September 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
PLAN DE PLAQUE


Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
PLAN DE PLAQUE

PSB1C3+E0240+J23102:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23104:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23105:
glycerol stock preparation from LB culture of the 17th September.

PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture of the 17th September.

PSB1C3+E0240+J23110:
Pre-culture does'nt grow. The TECAN measure is reported.

PSB1C3+E0240+J23115:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23116:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23117:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Sep 18

Retrieved from "http://2014.igem.org/Team:Evry/Interlab_Study/09-18-2014"