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Team:Evry/Interlab Study/09-18-2014
From 2014.igem.org
(Difference between revisions)
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<i>PSB1C3+E0240+J23101</i>: <br> | <i>PSB1C3+E0240+J23101</i>: <br> | ||
| - | + | <ul> | |
| + | <li>Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br> | ||
| + | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h). | ||
| + | </ul> | ||
| + | <img src="" alt="PLAN DE PLAQUE" /> | ||
| + | <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23102</i>: <br> | <i>PSB1C3+E0240+J23102</i>: <br> | ||
| - | + | Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br><br> | |
<br> | <br> | ||
<i>PSB1C3+E0240+J23104</i>: <br> | <i>PSB1C3+E0240+J23104</i>: <br> | ||
| - | + | Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br> | |
<br> | <br> | ||
| Line 44: | Line 49: | ||
<ul> | <ul> | ||
<li>glycerol stock preparation from LB culture from the 17th September. | <li>glycerol stock preparation from LB culture from the 17th September. | ||
| - | + | Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br> | |
</ul> | </ul> | ||
<br> | <br> | ||
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<i>PSB1C3+E0240+J23115</i>: <br> | <i>PSB1C3+E0240+J23115</i>: <br> | ||
| - | + | Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br> | |
<br> | <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23116</i>: <br> | <i>PSB1C3+E0240+J23116</i>: <br> | ||
| - | Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C. | + | Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br> |
<br> | <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23117</i>: <br> | <i>PSB1C3+E0240+J23117</i>: <br> | ||
| - | Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.<br> | + | Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.<br> |
<br> | <br> | ||
Revision as of 20:00, 19 September 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101:
- Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
- Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
PSB1C3+E0240+J23102:
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23104:
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23105:
- glycerol stock preparation from LB culture from the 17th September.
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture from the 17th September.
PSB1C3+E0240+J23110:
Pre-culture does'nt grow. The TECAN measure is reported.
PSB1C3+E0240+J23115:
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23116:
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23117:
Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Sep 18
