Team:Evry/Interlab Study/09-11-2014

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     <center>1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction.  Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. </center>
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     <center>GEL 1: 1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction.  Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. </center>
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     <center>1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction. </center>
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     <center>GEL 2: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction. </center>
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     <center>1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23105 construction.</center>
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     <center>GEL 3: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23105 construction.</center>
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Revision as of 18:06, 19 September 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101, PSB1C3+E0240+J23118, PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:
  • Transformation plate from the 10th September observation. There were 50 colonies for each constructions.
  • PCR colony of 8 colonies for each construction following protocol table 1 and 2. https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg
    IMAGE
    Symbol
    GEL 1: 1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction. Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction.
    IMAGE
    Symbol
    GEL 2: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction.
    IMAGE
    Symbol
    GEL 3: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23105 construction.
  • PCR purification of sample 1 and 4 with the were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
  • Preparation of samples to sequencing. N° XX
  • Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.

PSB1C3+E0240+J231xx:
Sep 11

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