"
Team:Evry/Interlab Study/09-11-2014
From 2014.igem.org
(Difference between revisions)
| Line 59: | Line 59: | ||
<div class="thumb tnone"> | <div class="thumb tnone"> | ||
<div class="thumbinner" style="width:502px;"> | <div class="thumbinner" style="width:502px;"> | ||
| - | <a href="https://static.igem.org/mediawiki/2014/ | + | <a href="https://static.igem.org/mediawiki/2014/8/8f/Gel106_107gfp.jpg" class="image"> |
<img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg" width="450px;" class="thumbimage"/> | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg" width="450px;" class="thumbimage"/> | ||
</a> | </a> | ||
Revision as of 18:00, 19 September 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101, PSB1C3+E0240+J23118, PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:
- Transformation plate from the 10th September observation. There were 50 colonies for each constructions.
- PCR colony of 8 colonies for each construction following protocol table 1 and 2.
https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg
1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction. Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction.
1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction. - PCR purification of sample 1 and 4 with the were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
- Preparation of samples to sequencing. N° XX
- Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.
PSB1C3+E0240+J231xx:
Sep 11
