Team:Oxford/biosensor construction

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<h1white>Lots and lots of info about construction </h1white>
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<h1white>Why these two plasmid backbones? </h1white>
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<h1white>Lots and lots of info about construction </h1white></div></a>
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<h1white>Why these two plasmid backbones?</h1white></div></a>
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<h1>Title about construction</h1>
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<li>The two plasmids are partitioned during cell division by different systems, thus an equal proportion of each plasmid is maintained in each new daughter cell. </li><br>
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<li>Different antibiotic resistances will allow us to select for cells that have taken up both plasmids by application of both antibiotics.</li><br>
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<li>The replication origins compatible with E.coli and pseudomonas strains.</li><br>
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<li>We have used two plasmids so that we can test each part in isolation before transforming them both into the same cell.</li>
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<h1>Second paragraph about construction</h1>
 
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Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction Lots and lots of info about construction
 
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Revision as of 08:48, 18 September 2014


Construction


In order to be able to use our model and to determine whether DcmR acts as a repressor or activator in the presence of DCM we designed the following two plasmid systems:
pOXON-2-dcmR-mCherry
pOXON-2-dcmR-mCherry
Oxford iGEM 2014
pSRK-Gm-pdcmAsfGFP
pSRK-Gm-pdcmAsfGFP
Oxford iGEM 2014
Why these two plasmid backbones?
Why these two plasmid backbones?
  • The two plasmids are partitioned during cell division by different systems, thus an equal proportion of each plasmid is maintained in each new daughter cell.

  • Different antibiotic resistances will allow us to select for cells that have taken up both plasmids by application of both antibiotics.

  • The replication origins compatible with E.coli and pseudomonas strains.

  • We have used two plasmids so that we can test each part in isolation before transforming them both into the same cell.