Team:Evry/Interlab Study/09-11-2014

From 2014.igem.org

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<h2>Construction n°1: PSB1C3 with I20260</h2>
 
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<br/><h2>Construction n°2: PSB1C3 with J23101-E1010</h2>
 
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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>
 
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         <li> Preparation of samples to sequencing.
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         <li> Preparation of samples to sequencing. N° XX
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        <li> Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.
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<br/> PSB1C3+E0240+J231xx:
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Revision as of 19:35, 17 September 2014

Picture


Constructions with other promoters


PSB1C3+E0240+J23101 and PSB1C3+E0240+J23118:
  • Transformation plate from the 10th observation. There were 50 colonies for each constructions.
  • PCR colony of 8 colonies for each construction following protocol table 1 and 2. https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg
    IMAGE
    Symbol
    1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction. Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction.
  • Preparation of samples to sequencing. N° XX
  • Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.

PSB1C3+E0240+J231xx: Sep 11

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