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Team:Evry/Interlab Study/09-11-2014
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<h2>Constructions with other promoters</h2> | <h2>Constructions with other promoters</h2> | ||
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| + | PSB1C3+E0240+J23101 and PSB1C3+E0240+J23118: | ||
| + | <ul> | ||
| + | <li> Transformation plate from the 10th observation. There were 50 colonies for each constructions. | ||
| + | <li> PCR colony of 8 colonies for each construction following protocol table 1 and 2. | ||
| + | https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg | ||
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| + | <a href="https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg" class="image"> | ||
| + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg" width="450px;" class="thumbimage"/> | ||
| + | </a> | ||
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| + | <a href="https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg" class="internal" title="Enlarge"> | ||
| + | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
| + | </a> | ||
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| + | <center>1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction. Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. </center> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <li> Preparation of samples to sequencing. | ||
| + | </ul> | ||
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<span class="cd-date">Sep 11</span> | <span class="cd-date">Sep 11</span> | ||
Revision as of 19:31, 17 September 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Constructions with other promoters
PSB1C3+E0240+J23101 and PSB1C3+E0240+J23118:
- Transformation plate from the 10th observation. There were 50 colonies for each constructions.
- PCR colony of 8 colonies for each construction following protocol table 1 and 2.
https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg
1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction. Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. - Preparation of samples to sequencing.
