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Revision as of 15:08, 15 September 2014 (view source) MelaCriq (Talk | contribs) (Created page with "== Lab Work== ''By Mélanie'' ===D- Lemon scent===") ← Older edit		Revision as of 16:23, 17 September 2014 (view source) MelaCriq (Talk | contribs) (→D- Lemon scent) Newer edit →
Line 3:		Line 3:
	===D- Lemon scent===		===D- Lemon scent===
		+	====PCR====
		+	I check the presence of the insert in the pPS2 (https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 here]
		+
		+	[photo]
		+
		+
		+	====pPS2ligation===
		+
		+	====plasmid extraction===
		+	LS/GS/CAD --> do sotck
		+
		+	GS(for pSBIC3)
		+
		+	====transformation====
		+	pPS2
		+	pPS3
		+	pPS4
		+
		+	cad in pSBIC3

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Revision as of 16:23, 17 September 2014

Contents 1 Lab Work 1.1 D- Lemon scent 1.1.1 PCR 1.2 =pPS2ligation 1.3 =plasmid extraction 1.3.1 transformation

Lab Work

By Mélanie

D- Lemon scent

PCR

I check the presence of the insert in the pPS2 (https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 here]

[photo]

=pPS2ligation

=plasmid extraction

LS/GS/CAD --> do sotck

GS(for pSBIC3)

transformation

pPS2 pPS3 pPS4

cad in pSBIC3