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Notebook

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very ustoeful organizational tool as well.

アメフラシはこちら

7/15

transformation

Tatsui, Matsumoto and Sukegawa

Name	Sample/(µl)	Competent Cells/(µl)	Total/(µl)	Medium
BBa_J23101(Pcon)	2	20(2/23)	22	LB(CP)
BBa_B0032(RBS)	2	20(2/23)	22	LB(CP)

7/16

transformation

Tatsui

Name	Sample/(µl)	Competent Cells/(µl)	Total/(µl)	Medium
BBa_B0030(1)	2	20(2/23)	22	LB(CP)
BBa_B0030(2)	2	20(bought)	22	LB(CP)
BBa_B0032(1)	2	20(2/23)	22	LB(CP)
BBa_B0032(2)	2	20(bought)	22	LB(CP)

7/17

Colony PCR

Tatsui, Matsumoto, Li, and Yamauchi

Name
7/16 BBa_B0030(1) Colony_1
7/16 BBa_B0030(1) Colony_2
7/16 BBa_B0030(1) Colony_3
7/16 BBa_B0030(2) Colony_1
7/16 BBa_B0030(2) Colony_2
7/16 BBa_B0030(2) Colony_3
7/16 BBa_B0032(1) Colony_1
7/16 BBa_B0032(1) Colony_2
7/16 BBa_B0032(1) Colony_3
7/16 BBa_B0032(2) Colony_1
7/16 BBa_B0032(2) Colony_2
7/16 BBa_B0032(2) Colony_3
Negative control

Liquid Culture

Tatsui, Matsumoto, Li, and Yamauchi

Name	medium
7/16 BBa_B0030(1) Colony_1	Plusgrow medium(CP)
7/16 BBa_B0030(1) Colony_2	Plusgrow medium(CP)
7/16 BBa_B0030(1) Colony_3	Plusgrow medium(CP)
7/16 BBa_B0030(2) Colony_1	Plusgrow medium(CP)
7/16 BBa_B0030(2) Colony_2	Plusgrow medium(CP)
7/16 BBa_B0030(2) Colony_3	Plusgrow medium(CP)
7/16 BBa_B0032(1) Colony_1	Plusgrow medium(CP)
7/16 BBa_B0032(1) Colony_2	Plusgrow medium(CP)
7/16 BBa_B0032(1) Colony_3	Plusgrow medium(CP)
7/16 BBa_B0032(2) Colony_1	Plusgrow medium(CP)
7/16 BBa_B0032(2) Colony_2	Plusgrow medium(CP)
7/16 BBa_B0032(2) Colony_3	Plusgrow medium(CP)

7/18

Miniprep

Tatsui, Matsumoto, Tsujii, Stephane, and Yamauchi

DNA	Concentration/(µg/ml)	260/280	260/230
7/16 BBa_B0030(1) Colony_1	229	1.96	1.40
7/16 BBa_B0030(1) Colony_2	209	1.95	1.50
7/16 BBa_B0030(1) Colony_3	108	2.12	2.12
7/16 BBa_B0030(2) Colony_1	-	-	-
7/16 BBa_B0030(2) Colony_2	174	2.02	1.56
7/16 BBa_B0030(2) Colony_3	158	2.01	1.68
7/16 BBa_B0032(1) Colony_1	179	2.00	2.06
7/16 BBa_B0032(1) Colony_2	-	-	-
7/16 BBa_B0032(1) Colony_3	221	1.90	1.37
7/16 BBa_B0032(2) Colony_1	225	1.89	1.37
7/16 BBa_B0032(2) Colony_2	270	1.98	1.63
7/16 BBa_B0032(2) Colony_3	194	1.89	1.33

7/30

Restriction Enzyme Digestion

No name

7/18 RBS Sample	Sample/(µl)	EcoR1/(µl)	Xba1/(µl)	Spe1/(µl)	Pst1/(µl)	Buffur/(µl)	BSA/(µl)	MilliQ/(µl)	TOTAL/(µl)
7/16 BBa_B0030(2) Colony_1	1	0.3	0	0	0	1(H)	0	7.7	10
7/16 BBa_B0030(2) Colony_1	1	0	0.3	0	0	1(M)	1	6.7	10
7/16 BBa_B0030(2) Colony_1	1	0	0	0.3	0	1(M)	0	7.7	10
7/16 BBa_B0030(2) Colony_1	1	0	0	0	0.3	1(H)	0	7.7	10
Control	1	0	0	0	0	1(M)	0	8	10

Electrophoresis for confirmation

No name

Lane	Restriction Enzyme Digestion Products
1	Product1
2	Product2
3	Product3
4	Product4
5	Product5
6	1kbp ladder

7/31

Transformation

Hashitani, Tatsui, Li and Yamauchi

Name	Sample/(µl)	Competent Cells/(µl)	Total/(µl)	Medium
BBa_E0240(RBS-GFP-DT)	2	20	22	LB(CP)
J23100(Pcon)	2	20	22	LB(AMP)

Liquid Culture

No name

Name	medium
7/31 J23100(Pcon) Colony_1	Plusgrow medium(AMP)
7/31 J23100(Pcon) Colony_2	Plusgrow medium(AMP)
7/31 J23100(Pcon) Colony_3	Plusgrow medium(AMP)
7/31 J23100(Pcon) Colony_4	Plusgrow medium(AMP)
7/31 J23100(Pcon) Colony_5	Plusgrow medium(AMP)
7/31 J23100(Pcon) Colony_6	Plusgrow medium(AMP)

Restriction Enzyme Digestion

Hashitani, Tatsui, Li and Yamauchi

7/18 RBS Sample	Sample/(µl)	EcoR1/(µl)	Spe1/(µl)	Pst1/(µl)	Xba1/(µl)	Buffur/(µl)	BSA/(µl)	MilliQ/(µl)	TOTAL/(µl)
BBa_B0032	5	0.3	0	0	0	1(H)	0	3.7	10
BBa_B0032	5	0	0.3	0	0	1(M)	0	3.7	10
BBa_B0032	5	0	0	0.3	0	1(H)	0	3.7	10
BBa_B0032	5	0	0	0	0.3	1(M)	1	2.7	10
Control	5	0	0	0	0	1(M)	0	4	10

8/1

Electrophoresis for confirmation

Honda, Li and Yamauchi

Lane	Restriction Enzyme Digestion Products
1	1kb ladder
2	Product1
3	Product2 Error
4	-
5	-
6	Product3
7	Product4
8	Product5

8/1

PCR

Tatsui

BBa_E0240(RBS-GFP-DT)	Volume
10×buffur	5μL
2mMdNTPs	5μL
25mM MgSO4	2μL
VF Primer	1.5μL
VR Primer	1.5μL
template	0.5μL
KOD-plus	1μL
MilliQ	33.5μL
TOTAL	50μL

Steps	Temparature	Time	cycle
Pre Denature	94℃	2min	1 time
Denature	94℃	15sec	30 cycle
Annealing	55℃	30sec	30 cycle
Extension	68℃	1min	30 cycle

DNA	Concentration/(µg/ml)	260/280	260/230
Product	80	1.91	1.78

8/2

Miniprep

Tatsui, Honda, Matsumoto, Yamauchi, Li, Tsujii, and Nakamura

DNA	Concentration/(µg/ml)	260/280	260/230
7/31 J23100(Pcon) Colony_1	145	2.11	3.09
7/31 J23100(Pcon) Colony_2	-	-	-
7/31 J23100(Pcon) Colony_3	160	2.01	3.13
7/31 J23100(Pcon) Colony_4	140	1.87	2.70
7/31 J23100(Pcon) Colony_5	105	2.39	5.90
7/31 J23100(Pcon) Colony_6	180	1.90	1.37

8/20

Transformation

Honda

Name	Sample/(µl)	Competent Cells/(µl)	Total/(µl)	Medium
7/31 BBa_E0240(RBS-aFD-DT)	2	20	22	LB(CP)
BBa_I732095	2	20	22	LB(CP)

8/21

Colony PCR

Shimazaki

Name
?
?
?
?

8/21

Electrophoresis for confirmation

?

Lane	?
1	1kb ladder
2	BBa_E0430-?
3	BBa_E0430-?
4	BBa_E0430-?
5	Negative Control

@@ Line 321: / Line 321: @@
 <table class="note">
 <tr><th>Name</th><th>Sample/(&micro;l)</th><th>Competent Cells/(&micro;l)</th><th>Total/(&micro;l)</th><th>Medium</th></tr>
-<tr><td>7/31 BBa_E0240(RBS-aFD-DT)</td><td>2</td><td>20</td><td>22</td><td>LB(?)</td></tr>
+<tr><td>7/31 BBa_E0240(RBS-aFD-DT)</td><td>2</td><td>20</td><td>22</td><td>LB(CP)</td></tr>
-<tr><td>BBa_I732095</td><td>2</td><td>20</td><td>22</td><td>LB(AMP)</td></tr>
+<tr><td>BBa_I732095</td><td>2</td><td>20</td><td>22</td><td>LB(CP)</td></tr>
 </table>
@@ Line 346: / Line 346: @@
 <tr><td>4</td><td>BBa_E0430-?</td></tr>
 <tr><td>5</td><td>Negative Control</td></tr>
-</table>
-<h2>8/22</h2>
-<h3>Miniprep</h3>
-<span class="author">Honda</span>
-<table class="note">
-<tr><th>Sample</th><th>density</th><th>260/280</th><th>260/230</th></tr>
-<tr><td>BBa_I732095-Sample1</td><td>430µg/mL</td><td>1.68</td><td>2.13</td></tr>
-<tr><td>BBa_I732095-Sample2</td><td>255µg/mL</td><td>1.87</td><td>1.81</td></tr>
-<tr><td>BBa_I732095-Sample3</td><td>215µg/mL</td><td>1.78</td><td>1.35</td></tr>
-</table>
-<h2>8/22</h2>
-<h3>Restriction Enzyme Digestion</h3>
-<span class="author">Nakamura</span>
-<table class="note">
-<tr><td>BBa_I732095-Sample1</td><td>4.7µL</td></tr>
-<tr><td>EcoRI</td><td>1µL</td></tr>
-<tr><td>SpeRI</td><td>1µL</td></tr>
-<tr><td>Buffur(H)</td><td>3µL</td></tr>
-<tr><td>MilliQ</td><td>20.3µL</td></tr>
-<tr><td>TOTAL</td><td>30µL</td></tr>
-<tr><td>BBa_I732095-Sample2</td><td>7.8µL</td></tr>
-<tr><td>EcoRI</td><td>1µL</td></tr>
-<tr><td>SpeRI</td><td>1µL</td></tr>
-<tr><td>Buffur(H)</td><td>3µL</td></tr>
-<tr><td>MilliQ</td><td>20.3µL</td></tr>
-<tr><td>TOTAL</td><td>30µL</td></tr>
-<tr><td>BBa_I732095-Sample3</td><td>9.3µL</td></tr>
-<tr><td>EcoRI</td><td>1µL</td></tr>
-<tr><td>SpeRI</td><td>1µL</td></tr>
-<tr><td>Buffur(H)</td><td>3µL</td></tr>
-<tr><td>MilliQ</td><td>15.7µL</td></tr>
-<tr><td>TOTAL</td><td>30µL</td></tr>
-</table>
-<h2>8/22</h2>
-<h3>Transformation</h3>
-<span class="author">Tatsui and Yamauchi</span>
-<table class="note">
-<tr><th>BBa_J04450(pSB3C5)</th></tr>
-<tr><td>DNA</td><td>2µL</td></tr>
-<tr><td>Conpitent cell</td><td>20µL</td></tr>
-</table>
-<h2>8/22</h2>
-<h3>Transformation</h3>
-<span class="author">Tatsui and Yamauchi</span>
-<table class="note">
-<tr><th>BBa_J04450(pSB3C5)</th><th>Time</th></tr>
-<tr><td>on ice</td><td>30min</td></tr>
-<tr><td>heat shock</td><td>45sec</td></tr>
-<tr><td>on ice</td><td>2min</td></tr>
-<tr><td>Preculture</td><td>1hour</td></tr>
-</table>
-<h2>8/25</h2>
-<h3>Transformation</h3>
-<span class="author">Tatsui, Kita and Li</span>
-<table class="note">
-<tr><th>BBa_J04450(2014)</th></tr>
-<tr><td>DNA</td><td>20µL</td></tr>
-<tr><td>Conpitent cell</td><td>40µL</td></tr>
-<tr><th>BBa_J04450(2013)</th></tr>
-<tr><td>DNA</td><td>20µL</td></tr>
-<tr><td>Conpitent cell</td><td>40µL</td></tr>
-<tr><th>BBa_C0051</th></tr>
-<tr><td>DNA</td><td>20µL</td></tr>
-<tr><td>Conpitent cell</td><td>40µL</td></tr>
-</table>
-<h2>8/25</h2>
-<h3>liquid culture</h3>
-<span class="author">Tatsui</span>
-<table class="note">
-<tr><th>8/22 J04450(psB3C5) 30mL</th></tr>
-</table>
-<h2>8/26</h2>
-<h3>Miniprep</h3>
-<span class="author">Tsujii, Honda and Matsumoto</span>
-<table class="note">
-<tr><th>Sample</th><th>density(µg/mL)</td><td>260/280</td><td>260/230</th></tr>
-<tr><td>J04450(psB3C5)</td><td>575</td><td>1.37</td><td>1.34</td></tr>
-</table>
-<h2>8/26</h2>
-<h3>liquid culture</h3>
-<span class="author">Tatsui, Matsumoto and Nakamura</span>
-<table class="note">
-<tr><th>8/25 psB2K3×5(30mL)</th></tr>
-<tr><th>8/25 psB1A2×2(12mL)</th></tr>
-</table>
-<h2>8/27</h2>
-<h3>Miniprep</h3>
-<span class="author">No name</span>
-<table class="note">
-<tr><th>Sample</th><th>density</th><th>260/280</th><th>260/230</th></tr>
-<tr><td>8/26 licuid culture psB2K3</td><td>1045(µg/mL)</td><td>1.43</td><td>1.11</td></tr>
-<tr><td>8/26 licuid culture psB1A2</td><td>790(µg/mL)</td><td>1.39</td><td>0.90</td></tr>
-</table>
-<h2>8/27</h2>
-<h3>BBa_J04450(psB3T5)</h3>
-<span class="author">No name</span>
-<table class="note">
-<tr><th>DNA</th><th>6mL</th></tr>
-<tr><th>Tet</th><th>60µg(10µg/mL)</th></tr>
-</table>
-<h2>8/29</h2>
-<h3>Miniprep</h3>
-<span class="author">Tatsui</span>
-<table class="note">
-<tr><th>DNA</th><th>Concentration(&micro;g/ml)</th><th>260/280</th><th>260/230</th></tr>
-<tr><td>7/16 BBa_B0030-(1)</td><td>229</td><td>1.96</td><td>1.40</td></tr>
-</table>
-<h3>transformation</h3>
-<span class="author">Tatsui</span>
-<table class="note">
-<tr><th>Name</th><th>Sample(&micro;l)</th><th>Competent Cells(&micro;l)</th><th>Total(&micro;l)</th><th>Plate</th></tr>
-<tr><td>BBa_J04450</td><td>2</td><td>20(2/23)</td><td>22</td><td>LB（CP）</td></tr>
-</table>
-<h2>9/1</h2>
-<h3>Colony PCR</h3>
-<span class="author">No name</span>
-<h3>Restriction Enzyme Digestion</h3>
-<span class="author">No name</span>
-<table class="note">
-<tr><th>Sample</th><th>Volume</th><th>EcoR1</th><th>Pst1</th><th>Buffur(H)</th><th>MilliQ</th><th>TOTAL</tr>
-<tr><td>8/28 pSB3T5</td><td>25</td><td>2</td><td>2</td><td>6</td><td>25</td><td>60</td></tr>
-<tr><td>Control 1</td><td>3.7</td><td>0</td><td>0</td><td>1</td><td>5.3</td><td>10</td></tr>
-<tr><td>8/22 I732095</td><td>14</td><td>3</td><td>3</td><td>9</td><td>61</td><td>90</td></tr>
-<tr><td>Control 2</td><td>1.5</td><td>0</td><td>0</td><td>1</td><td>7.5</td><td>10</td></tr>
-<tr><td>8/27 pSB2K3</td><td>6</td><td>3</td><td>3</td><td>9</td><td>69</td><td>90</td></tr>
-<tr><td>Control 3</td><td>1</td><td>0</td><td>0</td><td>1</td><td>8</td><td>10</td></tr>
-<tr><td>Pcon 3</td><td>20</td><td>1.5</td><td>1.5</td><td>5</td><td>17</td><td>45</td></tr>
-<tr><td>Control 4</td><td>4</td><td>0</td><td>0</td><td>1</td><td>5</td><td>10</td></tr>
-<tr><td>Pcon 1</td><td>20</td><td>1.5</td><td>1.5</td><td>5</td><td>17</td><td>45</td></tr>
-<tr><td>Control 5</td><td>4</td><td>0</td><td>0</td><td>1</td><td>5</td><td>10</td></tr>
-</table>
-<h3>Liquid Culture</h3>
-<span class="author">No name</span>
-<table class="note">
-<tr><th>Sample</th><th>Medium</th><th>Volume</th></tr>
-<tr><td>8/29 BBa_J04450(pSB4A5)</td><td>Plusgrow medium(+Amp)</td><td>12ml</td></tr>
-<tr><td>8/1 Pcon 1</td><td>Plusgrow medium(+Amp)</td><td>12ml</td></tr>
-<tr><td>8/27 pSB3T5 1</td><td>Plusgrow medium(+Tet)</td><td>12ml</td></tr>
-<tr><td>8/29 dddD+pSB1C3 1</td><td>Plusgrow medium(+CP)</td><td>3ml</td></tr>
-<tr><td>8/29 dddD+pSB1C3 2</td><td>Plusgrow medium(+CP)</td><td>3ml</td></tr>
-<tr><td>8/29 dddD+pSB1C3 3</td><td>Plusgrow medium(+CP)</td><td>3ml</td></tr>
 </table>
 </div>
 </html>

Team:Kyoto/Notebook

From 2014.igem.org

Revision as of 02:11, 17 September 2014

Notebook

7/15

transformation

7/16

transformation

7/17

Colony PCR

Liquid Culture

7/18

Miniprep

7/30

Restriction Enzyme Digestion

Electrophoresis for confirmation

7/31

Transformation

Liquid Culture

Restriction Enzyme Digestion

8/1

Electrophoresis for confirmation

8/1

PCR

8/2

Miniprep

8/20

Transformation

8/21

Colony PCR

8/21

Electrophoresis for confirmation