Team:Toulouse/Project/Overview

From 2014.igem.org

(Difference between revisions)
(Created page with "{{CSS/Main}} <html> <head> <!-- this section changes the default wiki template to a white full width background --> <style type="text/css"> - →Removing wiki-like stuff: /****...")
 
(13 intermediate revisions not shown)
Line 82: Line 82:
/* Barre de Menu*/
/* Barre de Menu*/
-
.btHeaderA{  
+
/* Les boutons*/
-
     position : relative;
+
 
-
     z-index:2;
+
a.btHeaderA1{
-
     width: 100%;
+
    color:#000000;
-
height: auto;
+
     position : absolute;
 +
        width: 100px;
 +
        height: 120px;
 +
     line-height:220px;
 +
     top : 30px;
 +
    background-image:url(https://static.igem.org/mediawiki/2014/0/0e/Bt_home_1.png);
 +
    background-position:center top;
 +
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;   
 +
}
 +
 
 +
a.btHeaderA1:hover{
 +
    background-image:url(https://static.igem.org/mediawiki/2014/9/96/Bt_home_2.png);
 +
    background-position:center top;
 +
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;
}
}
-
.btHeaderB{
+
.btHeaderA2{  
 +
    color:#000000;
     position : absolute;
     position : absolute;
-
     z-index:1;
+
        width: 100px;
-
     width: 100%;
+
        height: 120px;
-
height: auto;
+
     line-height:220px;
 +
     top: 5px;
 +
    background-image:url(https://static.igem.org/mediawiki/2014/a/a4/Bt_team_1.png);
 +
    background-position:center top;
 +
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;
}
}
-
.btHeaderC{
+
.btHeaderA2:hover{
-
     z-index:3;
+
     background-image:url(https://static.igem.org/mediawiki/2014/8/84/Bt_team_2.png);
-
     position: relative;
+
     background-position:center top;
-
    top : 45px;
+
     background-size: 100px 100px;
-
     text-align: center;
+
     background-repeat : no-repeat;
-
     width: auto;
+
-
height : auto;
+
-
   
+
}
}
-
.btHeaderA:hover{
+
.btHeaderA3{  
-
     z-index:1;
+
     color:#000000;
 +
    position : absolute;
 +
        width: 100px;
 +
        height: 120px;
 +
    line-height:220px;
 +
    top : 33px;
 +
    background-image:url(https://static.igem.org/mediawiki/2014/e/e2/Bt_project_1.png);
 +
    background-position:center top;
 +
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;
}
}
-
.btHeaderB:hover{
+
.btHeaderA3:hover{
-
     top : 0px;
+
     background-image:url(https://static.igem.org/mediawiki/2014/4/42/Bt_project_2.png);
-
     z-index : 3;
+
    background-position:center top;
 +
    background-size: 100px 100px;
 +
     background-repeat : no-repeat;
}
}
-
img.headerbutton{
+
.btHeaderA4{  
-
     max-width : 100px;
+
     color:#000000;
-
     width : 100%;
+
     position : absolute;
-
height : auto;
+
        width: 100px;
 +
        height: 160px;
 +
    line-height:220px;
 +
    top : 2px;
 +
    background-image:url(https://static.igem.org/mediawiki/2014/2/27/Bt_human_1.png);
 +
    background-position:center top;
 +
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;
 +
    white-space: nowrap;
}
}
-
img.headerbutton:hover{
+
.btHeaderA4:hover{
-
     max-width : 110px;
+
     background-image:url(https://static.igem.org/mediawiki/2014/e/ee/Bt_human_2.png);
-
     width : 100%;
+
     background-position:center top;
-
height : auto;
+
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;
}
}
 +
.btHeaderA5{
 +
    color:#000000;
 +
    position : absolute;
 +
        width: 100px;
 +
        height: 120px;
 +
    line-height:220px;
 +
    top : 38px;
 +
    background-image:url(https://static.igem.org/mediawiki/2014/d/d3/Bt_notebook_1.png);
 +
    background-position:center top;
 +
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;
 +
}
 +
 +
.btHeaderA5:hover{
 +
    background-image:url(https://static.igem.org/mediawiki/2014/c/c0/Bt_notebook_2.png);
 +
    background-position:center top;
 +
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;
 +
}
 +
 +
.btHeaderA6{
 +
    color:#000000;
 +
    position : absolute;
 +
        width: 100px;
 +
        height: 120px;
 +
    line-height:220px;
 +
    top: 30px;
 +
    background-image:url(https://static.igem.org/mediawiki/2014/6/6a/Bt_attributions_1.png);
 +
    background-position:center top;
 +
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;   
 +
}
 +
 +
.btHeaderA6:hover{
 +
    background-image:url(https://static.igem.org/mediawiki/2014/6/66/Bt_attributions_2.png);
 +
    background-position:center top;
 +
    background-size: 100px 100px;
 +
    background-repeat : no-repeat;
 +
}
 +
 +
 +
/*deroulement du menu*/
#Menu {
#Menu {
     position : absolute;
     position : absolute;
Line 154: Line 234:
     background: white; /* FIX IE7 : évite que la sous-liste ne disparaisse au moment où le curseur arrive au-dessus */
     background: white; /* FIX IE7 : évite que la sous-liste ne disparaisse au moment où le curseur arrive au-dessus */
     margin-top: -2px; /* Pour que le sous-menu recouvre son parent, facilitant le passage de la souris de l’un à l’autre */
     margin-top: -2px; /* Pour que le sous-menu recouvre son parent, facilitant le passage de la souris de l’un à l’autre */
-
}
+
    list-style-type: none;
 +
    list-style-image : url(#);
 +
    padding: 0;
 +
    margin-left: 1em;
 +
    left : 25px;
 +
    }
.sub-nav-item{
.sub-nav-item{
-
     background-color: transparent;
+
     background-image : url(https://static.igem.org/mediawiki/2014/7/72/Fond_sub_nav.png);
 +
    background-size: 100% 100%;
 +
    text-align : left;
}
}
.Item:hover .sub-nav {
.Item:hover .sub-nav {
     display: block; /* Affiche cette sous-liste au survol de son conteneur */
     display: block; /* Affiche cette sous-liste au survol de son conteneur */
-
     background-color: transparent;
+
     background-color: transparent;  
-
    top : 163px;
+
     Z-index : 4;  
-
     Z-index : 4;
+
}
-
     left : 25px;
+
 
 +
/*Sponsor (Home Page)*/
 +
#Sponsor{
 +
     width : 100%;
}
}
Line 183: Line 273:
position: relative;
position: relative;
overflow :hidden;
overflow :hidden;
 +
    top:250px;
     margin-top: 14px;
     margin-top: 14px;
     width: 970px;
     width: 970px;
Line 210: Line 301:
         position: absolute;
         position: absolute;
             left: -486px; /* ceil(963/2) + 74/2 = 482 + 37 = 519 */
             left: -486px; /* ceil(963/2) + 74/2 = 482 + 37 = 519 */
-
             top: 350px;
+
             top: 450px;
         margin-left: 50%;
         margin-left: 50%;
         cursor: pointer;
         cursor: pointer;
Line 222: Line 313:
         position: absolute;
         position: absolute;
             right: -488px;  
             right: -488px;  
-
             top: 350px;
+
             top: 450px;
         cursor: pointer;
         cursor: pointer;
         }
         }
Line 228: Line 319:
.disabled {
.disabled {
         visibility: hidden;}
         visibility: hidden;}
 +
       
 +
/*Main*/
 +
.Title{
 +
    text-shadow: 1px 3px 3px rgba(70, 156, 133, 0.75);
 +
    color: #2CC23E;
 +
    font-size: 50px;
 +
    text-align: center;
 +
}
-
/* Slider (thanks to flowplayer.org) */
+
.Sub_title{
-
    /*------- gallery ----------*/
+
     color: #2CC23E;
-
 
+
     font-size: 30px;
-
#slideshow {
+
-
     position:relative;
+
-
    height:391px;
+
-
     margin-top:20px;
+
}
}
-
#slideshow img {
+
.Article {
-
     position:absolute;
+
     text-align: justify;
-
    top:0;
+
     text-indent:15px;
-
    left:0;
+
-
     z-index:;
+
}
}
-
#slideshow img.active {
+
a.Link {
-
     z-index:2;
+
     font-size:10pt;
 +
    color :#A4A4A4;
 +
    text-decoration:none;
}
}
-
#slideshow img.last-active {
+
a.Link:hover{
-
     z-index:1;
+
     text-decoration:underline;
}
}
 +
/*Overview*/
 +
.CropImg{
 +
    position : absolute;
 +
    width: 700px;
 +
    left :50%;
 +
    margin-left:-350px;
 +
    height: auto;       
 +
    overflow: hidden;
 +
    display :block;
 +
}
 +
   
</style>
</style>
Line 275: Line 381:
<div id="Menu" style="background-color:transparent;">
<div id="Menu" style="background-color:transparent;">
-
 
     <div class="Item" style="left :5%;">   
     <div class="Item" style="left :5%;">   
-
         <a href="https://2014.igem.org/Team:Toulouse"style="color:#000000">
+
         <a class="btHeaderA1" href="https://2014.igem.org/Team:Toulouse">Home</a>
-
        <div class="btHeaderA" style="top : 30px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/0/0e/Bt_home_1.png"/></div>
+
-
        <div class="btHeaderB" style="top : 30px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/9/96/Bt_home_2.png"/></div>
+
-
        <div class="btHeaderC">Home</div>  
+
         </a>
         </a>
     </div>
     </div>
      
      
     <div class="Item" style="left :15%">  
     <div class="Item" style="left :15%">  
-
         <a href="https://2014.igem.org/Team:Toulouse/Team"style="color:#000000">  
+
         <a class="btHeaderA2" href="https://2014.igem.org/Team:Toulouse/Team">Team</a>
-
        <div class="btHeaderA" style="top:5px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/a/a4/Bt_team_1.png" /></div>
+
         <ul class="Item sub-nav" style="top : 125px;">
-
         <div class="btHeaderB" style="top:5px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/8/84/Bt_team_2.png" /></div>
+
-
        <div class="btHeaderC" align="center">Team</div>
+
-
        </a>
+
-
        <ul class="Item sub-nav">
+
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Team" class="leaf">Our Team</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Team" class="leaf">Our Team</a></li>
             <li class="sub-nav-item" align="left"><a href="https://igem.org/Team.cgi?year=2014&team_name=Toulouse" class="leaf"> Official Team Profile </a></li>
             <li class="sub-nav-item" align="left"><a href="https://igem.org/Team.cgi?year=2014&team_name=Toulouse" class="leaf"> Official Team Profile </a></li>
Line 299: Line 397:
     <div class="Item" style=" left :25%">   
     <div class="Item" style=" left :25%">   
-
         <a href="https://2014.igem.org/Team:Toulouse/Project"style="color:#000000">
+
         <a class="btHeaderA3" href="https://2014.igem.org/Team:Toulouse/Project">Project</a>
-
        <div class="btHeaderA" style="top : 33px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/e/e2/Bt_project_1.png"/></div>
+
         <ul class="Item sub-nav" style="top : 155px;">
-
         <div class="btHeaderB" style="top : 33px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/4/42/Bt_project_2.png"/></div>
+
-
        <div class="btHeaderC" align="center">Project</div>
+
-
        </a>
+
-
        <ul class="Item sub-nav">
+
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Project/Overview" class="leaf">Overview</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Project/Overview" class="leaf">Overview</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Project/Fixation" class="leaf">Fixation</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Project/Fixation" class="leaf">Fixation</a></li>
Line 316: Line 410:
      
      
     <div class="Item" style=" left :35%">   
     <div class="Item" style=" left :35%">   
-
         <a href="https://2014.igem.org/Team:Toulouse/Human_Practice"style="color:#000000">
+
         <a class="btHeaderA4" href="https://2014.igem.org/Team:Toulouse/Human_Practice">Human Practice</a>
-
        <div class="btHeaderA" style="top : 2px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/2/27/Bt_human_1.png"/></div>
+
         <ul class="Item sub-nav" style="top : 124px;">
-
        <div class="btHeaderB" style="top : 2px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/e/ee/Bt_human_2.png"/></div>
+
-
        <div class="btHeaderC" align="center" style="Top : 18px;">Human <br> Practice</div>
+
-
        </a>
+
-
         <ul class="Item sub-nav">
+
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Safety" class="leaf">Safety</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Safety" class="leaf">Safety</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Human_Practice/Ethique" class="leaf">Ethique</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Human_Practice/Ethique" class="leaf">Ethique</a></li>
Line 329: Line 419:
      
      
     <div class="Item" style=" left :45%" >   
     <div class="Item" style=" left :45%" >   
-
         <a href="https://2014.igem.org/Team:Toulouse/Notebook"style="color:#000000">  
+
         <a class="btHeaderA5" href="https://2014.igem.org/Team:Toulouse/Notebook">Notebook</a>
-
        <div class="btHeaderA" style="top : 38px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/d/d3/Bt_notebook_1.png" /></div>
+
         <ul class="Item sub-nav" style="top : 160px;">
-
         <div class="btHeaderB" style="top : 38px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/c/c0/Bt_notebook_2.png" /></div>
+
-
        <div class="btHeaderC" align="center">Notebook</div>
+
-
        </a>
+
-
        <ul class="Item sub-nav">
+
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Notebook/Calendar" class="leaf">Calendar</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Notebook/Calendar" class="leaf">Calendar</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Notebook/Protocols" class="leaf">Protocols</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Notebook/Protocols" class="leaf">Protocols</a></li>
Line 341: Line 427:
     <div class="Item" style=" left : 55%">  
     <div class="Item" style=" left : 55%">  
-
         <a href="https://2014.igem.org/Team:Toulouse/Attributions"style="color:#000000">  
+
         <a class="btHeaderA6" href="https://2014.igem.org/Team:Toulouse/Attributions">Attributions</a>
-
        <div class="btHeaderA" style="top : 48px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/6/6a/Bt_attributions_1.png"/></div>
+
         <ul class="Item sub-nav" style="top : 152px;">
-
         <div class="btHeaderB" style="top : 48px;"><img class="headerbutton" alt="header buton" src="https://static.igem.org/mediawiki/2014/6/66/Bt_attributions_2.png"/></div>
+
-
        <div class="btHeaderC" align="center">Attributions</div>
+
-
        </a>
+
-
        <ul class="Item sub-nav">
+
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Attributions" class="leaf">Attributions</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Attributions" class="leaf">Attributions</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Sponsors" class="leaf">Sponsors</a></li>
             <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Sponsors" class="leaf">Sponsors</a></li>
Line 357: Line 439:
         </a>
         </a>
     </div>
     </div>
-
 
</div>
</div>
-
 
<!--end navigation menu -->
<!--end navigation menu -->
-
 
-
<tr height="250px"></tr>
 
</table>
</table>
 +
<div class="centering">
 +
<br/>
 +
<br/>
 +
<br/>
 +
<br/>
 +
<br/>
 +
<br/>
 +
<br/>
 +
<br/>
 +
<br/>
 +
<br/>
<!--Entrer votre texte ici -->
<!--Entrer votre texte ici -->
 +
<!--Titre-->
-
<!--Nom du projet-->
+
<div class="Title"> Let's save our trees with SubtiTree !</div>  
-
<center>
+
<br/>
-
<img width="100px" src= "https://static.igem.org/mediawiki/2014/f/f5/Logo_essai_1_avec_texte.png"/>
+
<br/>
-
<p><center><font size="5" face=calibri color=green>
+
<img style="width:350px; float:right;" src="https://static.igem.org/mediawiki/2014/9/96/Canal1.png">
-
SubtiTree, a bacterium to save our trees
+
<br/>
-
</font></p>  
+
<div class="Article">
 +
    <p>
 +
Among many other things, Southern France is well-known for the gorgeousness of its landscapes. Plane trees (Platanus sp.) are widely present and participate to the charm of this area, especially along the famous “Canal du Midi”. It is impossible to imagine this UNESCO World Heritage masterpiece without its trees. Unfortunately, these trees are threatened by a severe fungal infection called canker, and today the only treatment consists in preventive tree-cutting which has a huge cost and implies significant ecological troubles.
 +
<p>
 +
Facing this emergency, the students from the iGEM team of Toulouse decided to be committed to the protection of their local heritage. Indeed, using a bacterium vector naturally present in the trees, our team offers an alternative solution originated from synthetic biology. Thanks to different genetic modules, the engineered bacterium called SubtiTree is first able to head towards the pathogen, then to bind to its cell wall in order to finally deliver different fungicides to save the tree from its invaders. Taking into account the ecological and ethical matters, our team thinks about how to limit the spreading of the optimized microorganism through different strategies.
 +
Although our project was born from a local problematic, it is transposable to other tree vascular diseases.
 +
</p>
 +
<p>
 +
       
 +
    </p>
 +
</div>
-
<!--Short description : à changer!!!-->
+
<div class="Article">
-
<table width="55%" height="10%">
+
    <p>
-
<tr><td><div align="justify"><font size="3" face=calibri color=black>
+
     
-
Fungal diseases lead to major economic losses all over the world.
+
    </p>
-
Some pathogens trigger trachemycosis by disturbing the vascular system of the plant.
+
</div>
-
Canker is one of these infections especially affecting plane trees (Platanus sp.). Plane trees are widely present in Southern France and particularly along the famous Canal du Midi, participating to the site gorgeousness.
+
<br/>
-
Today, the only treatment consists in preventive tree-cutting which has a huge cost and implies significant ecological troubles.
+
<div class="Sub_title">Chimiotactism <a href="https://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class ="Link">Show more</a></div>
-
Facing this emergency, our team offers an innovative alternative originated from synthetic biology.
+
<table width="100%"><tr><td bgColor="#097F09" height="1px"></td> </tr></table>
-
</font></div></td>
+
<br/>
-
<td align=center>
+
<div class="Article">
-
<img style="width:400px" ; src="https://static.igem.org/mediawiki/2014/9/96/Canal1.png">
+
    <p>
-
</td></tr>
+
        First, the bacterium targets the pathogen thanks to a chimiotactism module which recognizes the soluble chitin monomers (N-acetyl-glucosamine) released by the fungi.
-
<!--2eme ligne-->
+
    </p>
-
<tr><td align=center>
+
</div>
-
<img style="width:200px" ; src="https://static.igem.org/mediawiki/2014/7/72/Tronc_malade2.png">
+
<br/>
-
</td>
+
-
<td><div align="justify"><font size="3" face=calibri color=black>
+
-
The aim of our project is to make an endophytic bacterium capable of detecting the pathogen, and targeting it in order to deliver different fungicides to save the tree from its invaders.
+
-
Considering ecological and ethical matters, this project limits the spreading of our engineered bacterium in the environment.  
+
-
</font></div></td>
+
-
</tr>
+
-
</table>
+
    <div class="CropImg">
 +
    <img alt="chimiotactism"  style="position : relative; left:-2; top:-2; width: 704px; height:auto; display : block;" src="https://static.igem.org/mediawiki/2014/e/e6/GIF_chimiotactisme.gif"/>
 +
    </div>
 +
<div style="height:400px; position : relativee ; width : 135px; display : block;z-index:2;"></div>
 +
 +
<div class="Sub_title">Fixation <a href="https://2014.igem.org/Team:Toulouse/Project/Fixation" class ="Link">Show more</a></div>
 +
<table width="100%"><tr><td bgColor="#097F09" height="1px"></td> </tr></table>
 +
<br/>
 +
 +
<div class="Article">
 +
    <p>
 +
    Then, SubtiTree binds onto the pathogen by a chimeric protein anchored on one side to the bacterium pepdidoglycan and on the other side to the fungal chitin-based wall.
 +
    </p>
 +
</div>
 +
 +
    <div class="CropImg">
 +
    <img alt="Biding"  style="position : relative; left:-2; top:-2; width: 704px; height:auto;" src="https://static.igem.org/mediawiki/2014/f/fb/GIF_Fixation.gif"/>
 +
    </div>
 +
 +
<div style="height:400px; position : relativee ; width : 135px; display : block;z-index:2;"></div>
 +
 +
<div class="Sub_title">Fungicides <a href="https://2014.igem.org/Team:Toulouse/Project/Fungicides" class ="Link">Show more</a></div>
 +
<table width="100%"><tr><td bgColor="#097F09" height="1px"></td> </tr></table>
 +
<br/>
 +
 +
<div class="Article">
 +
    <p>
 +
        To finish, our designed bacterium fights against the pathogen by setting up a powerful treatment based on the production of three different fungicides.
 +
    </p>
 +
</div>
 +
 +
    <div class="CropImg">
 +
    <img alt="Fungicides"  style="position : relative; left:-2; top:-2; width: 704px; height:auto;" src="https://static.igem.org/mediawiki/2014/0/0c/GIF_Fongicides.gif"/>
 +
    </div>
 +
 +
 +
<div style="height:400px; position : relativee ; width : 135px; display : block;z-index:2;"></div>
 +
 +
<div class="Sub_title">Spreading <a href="https://2014.igem.org/Team:Toulouse/Project/Spreading" class ="Link">Show more</a></div>
 +
<table width="100%"><tr><td bgColor="#097F09" height="1px"></td> </tr></table>
 +
<br/>
 +
 +
<div class="Article">
 +
    <p>
 +
        Our team worked on different aspects to keep control on SubtiTree. The aim is to prevent horizontal transfers between different bacteria and to contain the engineered bacterium inside the tree during one season.
 +
    </p>
 +
</div>
 +
 +
    <div class="CropImg">
 +
    <img alt="Spreading"  style="position : relative; left:-2; top:-2; width: 704px; height:auto;" src="https://static.igem.org/mediawiki/2014/7/7a/GIF_Diss%C3%A9mination.gif"/>
 +
    </div>
 +
 
 +
<div style="height:400px; position : relativee ; width : 135px; display : block;z-index:2;"></div>
 +
 +
 +
<!--End of main-->
 +
<!--/* open Sans : fonnt Google*/-->
 +
 +
  <link href='http://fonts.googleapis.com/css?family=Open+Sans' rel='stylesheet' type='text/css'>
 +
 +
  <link href='http://fonts.googleapis.com/css?family=Open+Sans:400,600' rel='stylesheet' type='text/css'>
 +
 +
  <link href='http://fonts.googleapis.com/css?family=Open+Sans:800' rel='stylesheet' type='text/css'>
 +
 
<!-- Footer -->
<!-- Footer -->
Line 421: Line 578:
         </a>
         </a>
     </td>
     </td>
-
  </table>
+
</table>
 +
</div>
</div>
</div>
</html>
</html>

Latest revision as of 11:51, 12 September 2014

header background










Let's save our trees with SubtiTree !



Among many other things, Southern France is well-known for the gorgeousness of its landscapes. Plane trees (Platanus sp.) are widely present and participate to the charm of this area, especially along the famous “Canal du Midi”. It is impossible to imagine this UNESCO World Heritage masterpiece without its trees. Unfortunately, these trees are threatened by a severe fungal infection called canker, and today the only treatment consists in preventive tree-cutting which has a huge cost and implies significant ecological troubles.

Facing this emergency, the students from the iGEM team of Toulouse decided to be committed to the protection of their local heritage. Indeed, using a bacterium vector naturally present in the trees, our team offers an alternative solution originated from synthetic biology. Thanks to different genetic modules, the engineered bacterium called SubtiTree is first able to head towards the pathogen, then to bind to its cell wall in order to finally deliver different fungicides to save the tree from its invaders. Taking into account the ecological and ethical matters, our team thinks about how to limit the spreading of the optimized microorganism through different strategies. Although our project was born from a local problematic, it is transposable to other tree vascular diseases.


Chimiotactism Show more

First, the bacterium targets the pathogen thanks to a chimiotactism module which recognizes the soluble chitin monomers (N-acetyl-glucosamine) released by the fungi.


chimiotactism
Fixation Show more

Then, SubtiTree binds onto the pathogen by a chimeric protein anchored on one side to the bacterium pepdidoglycan and on the other side to the fungal chitin-based wall.

Biding
Fungicides Show more

To finish, our designed bacterium fights against the pathogen by setting up a powerful treatment based on the production of three different fungicides.

Fungicides
Spreading Show more

Our team worked on different aspects to keep control on SubtiTree. The aim is to prevent horizontal transfers between different bacteria and to contain the engineered bacterium inside the tree during one season.

Spreading

Retrieved from "http://2014.igem.org/Team:Toulouse/Project/Overview"