From 2014.igem.org
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| - | <u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </b> </u> <br>
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| - | We made a restriction control of our plasmid, and we tried to prove that these plasmids are present in our Pseudovibrio. <br>
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| - | After made a purification of plasmids in A1 and A2 with NucleoSpin Plamsid Kit (Macherey Nagel), we obtained concentrations at 380.1 ng/µL for pRhokHI-2 and at 91.1 ng/µL for pBBR1MCS.<br> <br>
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| - | We disgested these sample and our stock of pRhokHI-2 and pBBR1MCS with XbaI (unique restriction site on these plasmids) and we have make migrate the product of digestion. <br>
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| - | <a href="https://static.igem.org/mediawiki/2014/6/6a/XbaI.jpg" class="image">
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| - | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/6/6a/XbaI.jpg" width="500px;" class="thumbimage"/>
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| - | <a href="https://static.igem.org/mediawiki/2014/6/6a/XbaI.jpg" class="internal" title="Enlarge">
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| - | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
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| - | <center>Gel of digestion with XbaI</center>
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| - | <img src="https://static.igem.org/mediawiki/2014/6/6a/XbaI.jpg" alt="text to print if image not found" />
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Latest revision as of 17:44, 11 September 2014
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