From 2014.igem.org
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| - | <u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </b> </u> <br>
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| - | We made a restriction control of our plasmid, and we tried to prove that these plasmids are present in our Pseudovibrio. <br>
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| - | After made a purification of plasmids in A1 and A2 with NucleoSpin Plamsid Kit (Macherey Nagel), we obtained concentrations at 380.1 ng/µL for pRhokHI-2 and at 91.1 ng/µL for pBBR1MCS.<br> <br>
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| - | We disgested these sample and our stock of pRhokHI-2 and pBBR1MCS with XbaI (unique restriction site on these plasmids) and we have make migrate the product of digestion. <br>
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Latest revision as of 17:44, 11 September 2014
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