Team:ULB-Brussels/Project/Results
From 2014.igem.org
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<h1>New Results</h1> | <h1>New Results</h1> | ||
- | <p>We'll present the results week by week, in order to report the progressive advancement of our project as faithfully as possible. When possible, we'll use the structure of the WetLab & Methods section in order to clarify the link between each result and the part of the project it applies.</p> | + | <p>We'll present the results week by week, in order to report the progressive advancement of our project as faithfully as possible. When possible, we'll use the structure of the $\small WetLab & Methods$ section in order to clarify the link between each result and the part of the project it applies.</p> |
<h2> I. 30/06 – 06/07 </h2> | <h2> I. 30/06 – 06/07 </h2> | ||
• All the preliminary steps (brainstorming, organisation of the project,etc.) have already been done officiously during the year with the help of our supervisors.</p> | • All the preliminary steps (brainstorming, organisation of the project,etc.) have already been done officiously during the year with the help of our supervisors.</p> | ||
- | • | + | • 1$^{st}$ meeting in order to organize the search of the sponsors, the creation of the wiki, and the completion of the safety form.</p> |
• A sponsoring folder has been written in French by the sponsoring team, and a first list of biotech companies and public services susceptible of offering us sponsorship has been made.</p> | • A sponsoring folder has been written in French by the sponsoring team, and a first list of biotech companies and public services susceptible of offering us sponsorship has been made.</p> | ||
<h2> II. 07/07 – 13/07 </h2> | <h2> II. 07/07 – 13/07 </h2> | ||
- | • | + | • 2$^{nd}$ meeting to make a briefing of the coming manipulations and to find some ideas of Human Practice. Research of sponsorship is intensified.</p> |
• Familiarization with the lab and the first basic manipulations (preparation of media, dyalisis, electroporation) as well as presentation of the safe lab practices. </p> | • Familiarization with the lab and the first basic manipulations (preparation of media, dyalisis, electroporation) as well as presentation of the safe lab practices. </p> | ||
• Extraction of the biobricks we will use in our manipulation for cloning.</p> | • Extraction of the biobricks we will use in our manipulation for cloning.</p> | ||
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+ | • The electrocompetent bacteria used for the cloning are MC1061 $\small E.Coli$.</p> | ||
+ | • Spectrophotometric verification of the well-functioning of the RFP and GFP. The chosen biobrick of GFP had no promoter, we have to fuse it with a promoter.</p> | ||
+ | • Conservation of the transformed bacteria at -80°C (in glycerol).</p> | ||
+ | • Miniprep of the RFP and GFP biobricks, midiprep of the vector AraC-pbad33 (which we will use to make our constructions).</p> | ||
Revision as of 09:59, 11 September 2014
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$
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