"
Team:Evry/Notebook/Transformation/09-02-2014
From 2014.igem.org
(Difference between revisions)
| Line 10: | Line 10: | ||
<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results </b> </u> <br> | <u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results </b> </u> <br> | ||
| - | <img src="" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br> | + | <li> Positive control with E.Coli : <br> |
| + | <img src="https://static.igem.org/mediawiki/2014/0/05/ColiTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br> | ||
| + | |||
| + | <li> Resulst with Pseudovibrio : <br> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/f/f5/PlatesPseudoTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br> | ||
| + | NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old. <br> | ||
| + | A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000. | ||
| + | On Chloramphenicol, the contamination does'nt seem to grow. <br> | ||
| + | We have to prove that it's Pseudovibrio wich growth on Chloramphenicol. | ||
| + | |||
</p> | </p> | ||
Revision as of 13:22, 9 September 2014
Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results
NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old.
A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000. On Chloramphenicol, the contamination does'nt seem to grow.
We have to prove that it's Pseudovibrio wich growth on Chloramphenicol. Sep 02
