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Team:Evry/Notebook/Sensing/PCBs/08-15-2014
From 2014.igem.org
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| + | <b><u>Survival test on E.coli BL21:</u></b> | ||
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<li>Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L | <li>Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L | ||
<br/><img src="https://static.igem.org/mediawiki/2014/9/95/Dilution_compose.jpg" alt="image not found"/> | <br/><img src="https://static.igem.org/mediawiki/2014/9/95/Dilution_compose.jpg" alt="image not found"/> | ||
| - | <li> 300µL of E.coli BL21 | + | <li> 300µL of E.coli BL21 were added in each eppendorf tube. |
<br/> We had 2 control tubes: | <br/> We had 2 control tubes: | ||
<br/><ul> | <br/><ul> | ||
| - | <li> A negative control | + | <li> A negative control which contains 2,7mL of M9 medium + 300µL of compound |
| - | <li> A positive control | + | <li> A positive control which contains 2,7mL of M9 medium + 300µL of E.coli BL21 |
</ul> | </ul> | ||
| - | <li> | + | <br/> |
| + | <li> Tubes were incubated at 37°C overnight, 200rpm | ||
</p> | </p> | ||
Latest revision as of 10:48, 6 September 2014
Survival test on E.coli BL21:
- Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
- 300µL of E.coli BL21 were added in each eppendorf tube.
We had 2 control tubes:- A negative control which contains 2,7mL of M9 medium + 300µL of compound
- A positive control which contains 2,7mL of M9 medium + 300µL of E.coli BL21
- Tubes were incubated at 37°C overnight, 200rpm Aug 15
