Team:UCL/about

From 2014.igem.org

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                 <h2>Project Overview</h2>
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                 <h1>Experiments</h1>              
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                <h4>The Problem: Azo Dyes in the Environment</h4>
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                <p><img class="alignright" src="https://static.igem.org/mediawiki/2014/2/2d/Screen-shot-2013-04-13-at-2-25-18-pm.png" alt=" ">Azo dyes are the main synthetic colourant used in the industrial manufacture of a wide range of products such as clothing, upholstery, cosmetics, tattoo ink and more. These dyes are widely known to be safe and stable forms of synthetic colourants, however, when they are broken down in the guts of organisms they take on dangerous properties.  In industry, leftover dye effluent is often not properly disposed of, or removed, during water treatment, which results in the accumulation of azo dyes in water bodies. It is at this point that these excess dyes are ingested, broken down, and excreted as products that have been found to be mutagenic and carcinogenic. Despite such toxicity, little to no effort has been made to dispose of these leftover azo dyes more responsibly.</p>
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<h4>Experiment 1: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit</h4>
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<div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr></div>
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<br/><p><strong>Protocols&nbsp;&nbsp;</strong>
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<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
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<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
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<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
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<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
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<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
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<p>senectus et netus et malesuada</p>
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                <h4>The Solution: Goodbye AzoDyes, UCL iGEM Team 2014</h4>
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<h4>Experiment 2: Transforming <i>E. coli</i> with Azo-reductase plasmids</h4>
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                <p><img class="alignleft" src="https://static.igem.org/mediawiki/2014/3/36/Decolorization_2.jpg" alt=" ">Our iGEM project 2014 will work towards controllably degrading and detoxifying the excess azo dye effluent at the source - the textile factories - and filtering the different toxic breakdown products elsewhere, before they ever reach the water systems. Our aim is to then convert these products into innoculous, and potentially useful, chemicals that can be used in other processes. In effect, we want to recycle and re-use the excess azo dyes. </p>
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<div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr></div>
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<br/><p><strong>Protocols&nbsp;&nbsp;</strong>
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<a href="/Team:UCL/protocols"><span class="label label-warning">primer design</span></a>
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<a href="/Team:UCL/protocols"><span class="label label-warning">PCR</span></a>
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<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
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<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
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<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
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<p>senectus et netus et malesuada</p>
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                <h4>Using Synthetic Biology</h4>
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<h4>Experiment 3: Diagnostic digest of azo-reductase plasmids</h4>
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                <p>To do this will involve creating an enhanced azo dye decolourising organism by introducing the genes for three enzymes related to the degradation of these dyes: azoreductase, laccase, and lignin peroxidase into a host <i>E.coli</i> cell. In an industrial context, these three enzymes would work sequentially in a bioreactor of changing conditions. First, azoreductase will cleave the azo bond (N=N) by a double reduction using NADPH as a cofactor; this will produce a series of highly toxic aromatic amines. These compounds will be then oxidised by incorporation of lignin peroxidase and laccase, completing decolourisation and decreasing toxicity levels to the point that the final products of the process are less toxic than the intact dyes themselves. The complementary action of azoreductase and lignin peroxidase will be studied in order to find out the best possible approach of sequential reaction, and this core degradation module will be extrapolated to other areas such as BioArt projects and work on algal-bacterial symbiosis, trying to set up the foundations for a synthetic ecology.</p>
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<div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013, 8:21 PM">October 15, 2013</abbr></div>
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                <p><figure> <img src="https://static.igem.org/mediawiki/2014/f/f2/Azo_reductase.jpg" alt="" class="aligncenter">
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<br/><p><strong>Protocols&nbsp;&nbsp;</strong>
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                <figcaption></figcaption>
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<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
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                </figure></p>
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<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
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<p>senectus et netus et malesuada</p>
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                <h4>Implementation in Industry</h4>
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<h4>Experiment 4: Creation of azo-reductase BioBrick parts from plasmids</h4>
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<div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr></div>
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                <p><figure> <img width=350 src="https://static.igem.org/mediawiki/2014/f/f7/BIoprocess.png" alt="" class="alignright"></figure>In the textile industry today, annual production of dyestuff amounts to millions of tons globally. Azo dyes represent two thirds of this value, a majority of which find their way to wastewater.
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<br/><p><strong>Protocols&nbsp;&nbsp;</strong>
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<br><br>
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<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
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Our idea is to conceive an integrated end-of-pipe method for detoxifying effluent streams of dye factories. The goal is to achieve a two-stage regimen in sequence to ensure optimal conditions for the degradation of azo dyes within a batch bioreactor system. This would be an attractive and effective approach to dealing with azo dye contamination of the environment. As a financial incentive, we are also looking at maximizing the profitability of various potential breakdown products. As a lucrative continuous-process alternative, we are investigating the application of microbial fuel cell technology to an aerobic bioreactor system, for simultaneously detoxifying azo dyes and generating electricity.
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<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
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<br><br>
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<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
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Considering the potential for scalability, this method would present various economic and environmental advantages for industries that generate large amounts of dyestuff. This could also be spinned to become a modular bioprocess method for wastewater treatment of other toxic, normally recalcitrant chemicals. </p>
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<p>senectus et netus et malesuada</p>
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                <h4>Recycling Azo Dyes</h4>
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<h4>Experiment 5: Diagnostic digest of azo-reductase BioBrick parts</h4>
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                <p>Depending on the azo dye that is being degraded, different sets of breakdown products can be produced. Once degraded, each of the different compounds will be identified and separated. We have three potential avenues for re-using these products: (1) converting functional groups on the aromatic amines into simple aromatic fragrances; (2) isolating and diverting nitrogenous compounds to algae, which can form, and maintain, a symbiotic relationship with the dye-degrading <em>E. coli</em> hosts; (3) selling more complex compounds to pharmaceutical companies for the production of drugs.</p>
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<div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013, 8:21 PM">October 15, 2013</abbr></div>
 +
<br/><p><strong>Protocols&nbsp;&nbsp;</strong>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
 +
<p>senectus et netus et malesuada</p>
 +
<h4>Experiment 6: Assembling azo-reductase BioBrick Device(s)</h4>
 +
<div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr></div>
 +
<br/><p><strong>Protocols&nbsp;&nbsp;</strong>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
 +
<p>senectus et netus et malesuada</p>
 +
 +
<h4>Experiment 7: Characterisation of azo-reductase BioBrick devices</h4>
 +
<div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr></div>
 +
<br/><p><strong>Protocols&nbsp;&nbsp;</strong>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
 +
<p>senectus et netus et malesuada</p>
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Revision as of 15:03, 5 September 2014

Goodbye Azo Dye : iGEM 2014 - University College London

 

About our project

Experiments

Experiment 1: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit

Adam Denyer    October 15, 2013

Protocols   competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 2: Transforming E. coli with Azo-reductase plasmids

Adam Denyer    October 15, 2013

Protocols   primer design PCR competent cells transformation miniprep

senectus et netus et malesuada

Experiment 3: Diagnostic digest of azo-reductase plasmids

Adam Denyer    October 15, 2013

Protocols   digest gel

senectus et netus et malesuada

Experiment 4: Creation of azo-reductase BioBrick parts from plasmids

Adam Denyer    October 15, 2013

Protocols   competent cells transformation miniprep

senectus et netus et malesuada

Experiment 5: Diagnostic digest of azo-reductase BioBrick parts

Adam Denyer    October 15, 2013

Protocols   digest gel

senectus et netus et malesuada

Experiment 6: Assembling azo-reductase BioBrick Device(s)

Adam Denyer    October 15, 2013

Protocols   competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 7: Characterisation of azo-reductase BioBrick devices

Adam Denyer    October 15, 2013

Protocols   competent cells transformation miniprep digest gel

senectus et netus et malesuada

Contact Us

University College London - Gower Street - London - WC1E 6BT - Biochemical Engineering Department
phone: +44 (0)20 7679 2000
email: ucligem2014@gmail.com

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