Team:ETH Zurich/labblog/20140825meet
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* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. | * We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. | ||
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+ | Diffusion experiment with agar. Check <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details. | ||
+ | </center> | ||
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- | < | + | Diffusion experiment with liquid. Check <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details. |
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|poster=<html>https://static.igem.org/mediawiki/2014/a/a9/ETH_Zurich_Diffusion3.JPG</html>}} | |poster=<html>https://static.igem.org/mediawiki/2014/a/a9/ETH_Zurich_Diffusion3.JPG</html>}} | ||
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+ | Diffusion experiment with beads. Check <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details. | ||
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|poster=<html>https://static.igem.org/mediawiki/2014/2/27/ETH_Zurich_Diffusion4.JPG</html>}} | |poster=<html>https://static.igem.org/mediawiki/2014/2/27/ETH_Zurich_Diffusion4.JPG</html>}} | ||
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Revision as of 10:35, 5 September 2014
Week 14
Monday, August 25th
- We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
Diffusion experiment with agar. Check <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details.
Diffusion experiment with liquid. Check <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details.
Diffusion experiment with beads. Check <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details.
- We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.
- We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.
Fitting of quorum sensing dynamic parameters. Check the corresponding article for more details.