Team:ETH Zurich/labblog/20140825meet

From 2014.igem.org

(Difference between revisions)
(→Week 14)
(→Week 14)
Line 6: Line 6:
* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
-
<html>
 
-
<div class="center">
 
-
<div class="thumb tnone">
 
-
<div class="thumbinner" style="width:462px;">
 
</html>
</html>
{{:Team:ETH Zurich/Templates/Video|
{{:Team:ETH Zurich/Templates/Video|
Line 18: Line 14:
|poster=<html>https://static.igem.org/mediawiki/2014/3/33/ETH_Zurich_Diffusion2.JPG</html>}}
|poster=<html>https://static.igem.org/mediawiki/2014/3/33/ETH_Zurich_Diffusion2.JPG</html>}}
<html>
<html>
-
Diffusion experiment with agar. Check  <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a>  for more details.
 
-
</div>
 
-
</div>
 
-
</div>
 
-
</html> {{:Team:ETH Zurich/Templates/Video|
 
-
width=460px|
 
-
id=video4|
 
-
ratio=143/100|
 
-
srcOGG=<html>https://static.igem.org/mediawiki/2014/2/2c/ETH_Zurich_diffusion_agar_s24.ogg</html>
 
-
|poster=<html>https://static.igem.org/mediawiki/2014/3/33/ETH_Zurich_Diffusion2.JPG</html>}}
 
-
<html>
 
<div class="center">
<div class="center">

Revision as of 09:57, 5 September 2014

Week 14

Monday, August 25th

  • We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.

</html>

Diffusion experiment with liquid. Check the corresponding article for more details.
Diffusion experiment with beads. Check the corresponding article for more details.


  • We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.
Comsol simulation of diffusion experiments for 1mM and for 1 μM AHL initial concentration in sender wells. It explains why with 1mM all wells start glowing at the same time.
  • We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.
Fitting of quorum sensing dynamic parameters. Check the corresponding article for more details.

top

Retrieved from "http://2014.igem.org/Team:ETH_Zurich/labblog/20140825meet"