Team:TCU Taiwan
From 2014.igem.org
Line 93: | Line 93: | ||
<td width="20%"> </td> | <td width="20%"> </td> | ||
<td align="center"><p><font face="Trebuchet MS" size="6" color="#9FE0F6"><img src="https://static.igem.org/mediawiki/2014/f/f4/TCU-Project-logo.png" width="65"> Project Overview</font></p> | <td align="center"><p><font face="Trebuchet MS" size="6" color="#9FE0F6"><img src="https://static.igem.org/mediawiki/2014/f/f4/TCU-Project-logo.png" width="65"> Project Overview</font></p> | ||
- | <p align="left"><font size="3" face="Verdana" color="#F3F0CD" >Bacterial resistance to antibiotics has been a problem for public health, and the problem is getting worse.We are trying to develop a CRISPR system combined with phage transduction to solve this problem.<br><br>The type II CRISPR system is a powerful | + | <p align="left"><font size="3" face="Verdana" color="#F3F0CD" >Bacterial resistance to antibiotics has been a problem for public health, and the problem is getting worse.We are trying to develop a CRISPR system combined with phage transduction to solve this problem.<br><br> |
+ | This year, we are trying to develop a CRISPR system combined with phage transduction to solve bacteria's resistance to antibiotics. The type II CRISPR system is a powerful mechanism in gene engineering. This system has been proved to have more efficiency than other mechanisms such as zinc finger or TALEN. However CRISPR can only function within one cell, so we use M13 phage as vector to send our CRISPR into bacteria cells to cure their resistance for M13 is a lysogenic phage. In order to regulate our phage transduction, we use phagemid pBluescript and M13KO7 helper phage. The pBluescript contains our designed CRISPR system, it will produce CRISPR-transferd M13 phage only when a helper phage infect bacterium which have pBluescript. Then the engineered M13 phage will infect other bacteria and induce transduction of CRISPR system. Once we start the CRISPR, it will recognize and knock down the antibiotic resistance gene.</font></p> | ||
<p align="right"><a href="https://2014.igem.org/Team:TCU_Taiwan/Project"><img src="https://static.igem.org/mediawiki/2014/9/91/TCU-More-box.png" width="150px"></a></p></td> | <p align="right"><a href="https://2014.igem.org/Team:TCU_Taiwan/Project"><img src="https://static.igem.org/mediawiki/2014/9/91/TCU-More-box.png" width="150px"></a></p></td> | ||
<td width="20%"> </td> | <td width="20%"> </td> |
Revision as of 08:10, 5 September 2014
This Website currently under construction!Welcome to our new Internet domain!
Or go to Our Blog and Facebook
|
Project Overview Bacterial resistance to antibiotics has been a problem for public health, and the problem is getting worse.We are trying to develop a CRISPR system combined with phage transduction to solve this problem. |
|
||
|
||
|
||
|
powered by MediaWiki
|
Like & share them on Facebook Connect With Us |
Best for 1920x1080 with Chrome |