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Team:Paris Saclay/Notebook/September/3
From 2014.igem.org
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| - | Electrophoresis of the PCR made [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/2# | + | Electrophoresis of the PCR made [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/2#Limonene_synthase_PCR Yesterday] |
[photo] | [photo] | ||
Revision as of 15:45, 4 September 2014
Contents |
LabWork
B- Construction of the fusion protein
We made a classic exttraction of plasmids from the liquid cultures.
Check by electrophoresis if it worked and send it for sequencing.
D- Lemon scent
by Mélanie
PCR LS
Electrophoresis of the PCR made Yesterday
[photo]
The PCR have success so I use the PCR clean up kit to purify it
[photo] Electrophoresis after purification
=Ligation
We already have some pPSI digested and dephosphorelated
So I do a ligation:
