Team:WashU StLouis/Protocol
From 2014.igem.org
(Difference between revisions)
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<div class="floating-menu"> | <div class="floating-menu"> | ||
<h3>Protocols</h3> | <h3>Protocols</h3> | ||
- | <a href="#1"> | + | <a href="#1">PCR</a> |
- | <a href="#2"> | + | <a href="#2">Colony PCR</a> |
+ | <a href="#3">Gel Electrophoresis</a> | ||
+ | <a href="#4">DNA Purification</a> | ||
+ | <a href="#5">Digestion/Ligation</a> | ||
+ | <a href="#6">Transformation</a> | ||
+ | <a href="#7">Starting Cultures</a> | ||
+ | <a href="#8>Miniprep</a> | ||
</div> | </div> | ||
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<table id="general"> | <table id="general"> | ||
<tr> <td> | <tr> <td> | ||
+ | <a name="1"></a> | ||
<center><h1> Brauer Group Protocols </h1></center> | <center><h1> Brauer Group Protocols </h1></center> | ||
+ | <br> | ||
<a name="1"></a> | <a name="1"></a> | ||
<p><h3>Polymerase Chain Reaction</h3></p> | <p><h3>Polymerase Chain Reaction</h3></p> | ||
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<br> | <br> | ||
+ | <a name="2"></a> | ||
<p><h3>Colony PCR</h3></p> | <p><h3>Colony PCR</h3></p> | ||
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<p>Increase Annealing Time to 30 seconds</p> | <p>Increase Annealing Time to 30 seconds</p> | ||
- | + | <br> | |
- | < | + | <a name="3"></a> |
- | + | ||
<p><h3>Making a Gel for Gel Electrophoresis</h3></p> | <p><h3>Making a Gel for Gel Electrophoresis</h3></p> | ||
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<br> | <br> | ||
- | + | <a name="4"></a> | |
<p><h3>DNA Purification</h3></p> | <p><h3>DNA Purification</h3></p> | ||
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<br> | <br> | ||
- | + | <a name="5"></a> | |
<p><h3>Golden Gate Digestion/Ligation</h3></p> | <p><h3>Golden Gate Digestion/Ligation</h3></p> | ||
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<br> | <br> | ||
- | + | <a name="6"></a> | |
<p><h3>Transformation (Electroporation)</h3></p> | <p><h3>Transformation (Electroporation)</h3></p> | ||
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<br> | <br> | ||
- | + | <a name="7"></a> | |
<p><h3>Plating</h3></p> | <p><h3>Plating</h3></p> | ||
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<br> | <br> | ||
- | + | <a name="8"></a> | |
<p><h3>Plasmid Miniprep</h3></p> | <p><h3>Plasmid Miniprep</h3></p> | ||
Revision as of 03:38, 4 September 2014